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Herein, a three-dimensional biomimetic screening strategy was established. Cells with high \u03b11A-adrenergic receptor (AR) expression were employed as the screening tool and incorporated into a three-dimensional agarose network to prepare a biomimetic \u03b11A AR stationary phase. This screening strategy involves the incorporation of a large number of cells into a three-dimensional network, followed by analysis and identification based on the specific interactions between membrane receptors and their ligands. Importantly, the native cellular architecture and membrane receptor integrity were preserved within the biomimetic microenvironment. Screening of Curcuma longa extracts using the proposed strategy led to the identification of ar-turmerone, curcumin, and curcumin III as \u03b11A AR-binding candidates. Subsequent bioassays demonstrated their prostate relaxation and antiproliferative activities. This work establishes a biomimetic strategy for receptor-guided ligand screening from complex natural products and expands the toolbox of cell-based bioanalytical platforms for ligand discovery.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03264","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:13:43Z","timestamp":1785129223000},"page":"23252-23265","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Affinity\nScreening Approach for Bioactive\nCompounds in\n                    <i>Curcuma longa<\/i>\n                    Utilizing a Cell-Functionalized\nHydrogel"],"prefix":"10.1021","volume":"98","author":[{"given":"Meihui","family":"Zhang","sequence":"first","affiliation":[{"name":"Xi\u2019an Jiaotong University , , ,","place":["Xi\u2019an, China, 710061"]},{"name":"Shaanxi Engineering Research Center of Cardiovascular Drugs Screening & Analysis , ,","place":["Xi\u2019an, China, 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