{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:25:08Z","timestamp":1787063108616,"version":"build-2736575974"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100013145","name":"Primary Research and Development Plan of Zhejiang Province","doi-asserted-by":"publisher","award":["2025C02190"],"award-info":[{"award-number":["2025C02190"]}],"id":[{"id":"10.13039\/501100013145","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013145","name":"Primary Research and Development Plan of Zhejiang Province","doi-asserted-by":"publisher","award":["2026C02A1160"],"award-info":[{"award-number":["2026C02A1160"]}],"id":[{"id":"10.13039\/501100013145","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022B1515230005"],"award-info":[{"award-number":["2022B1515230005"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Provincial Science and Technology Support Program","doi-asserted-by":"publisher","award":["2025YFHZ0071"],"award-info":[{"award-number":["2025YFHZ0071"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Small extracellular vesicles (sEVs) have emerged as promising biomarkers for liquid biopsy; however, the limited specificity of single-marker detection and the intrinsic heterogeneity of circulating sEVs hinder their reliable clinical application. Herein, we develop a programmable dual-aptamer amplified luminescent proximity homogeneous assay (AlphaLISA) for rapid and wash-free detection of tumor-derived sEVs. In this strategy, EpCAM and PD-L1 aptamers simultaneously recognize two membrane proteins on the same vesicle, enabling proximity-induced interaction between donor and acceptor beads to generate a chemiluminescent signal via singlet oxygen-mediated energy transfer. This dual-recognition design effectively improves analytical specificity by implementing a molecular \u201cAND\u201d logic requirement for signal generation. The proposed assay exhibits a broad linear range of 5.3 \u00d7 105 to 5.3 \u00d7 109 particles mL\u20131 with a limit of detection of 1.63 \u00d7 104 particles mL\u20131. Good analytical performance was demonstrated by recovery rates of 93.9\u2013107.5% with relative standard deviations below 5%, indicating high accuracy and reproducibility. Notably, the assay enables signal readout within 1 min under homogeneous conditions, highlighting its operational simplicity and rapid response. Clinical applicability was evaluated using serum samples from 20 lung cancer patients and 20 healthy controls, achieving an area under the ROC curve of 0.87 (95% CI: 0.75\u20130.97), with a sensitivity of 95% and a specificity of 75%. Overall, this programmable dual-aptamer AlphaLISA platform provides a rapid, homogeneous, and highly adaptable strategy for tumor-derived sEVs analysis, offering promising potential for noninvasive cancer diagnostics<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03310","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:38:31Z","timestamp":1785854311000},"page":"23806-23818","source":"Crossref","is-referenced-by-count":0,"title":["Logic-Gated\nDual-Aptamer Proximity AlphaLISA for Rapid\nand Sensitive Detection of Tumor-Derived Small Extracellular Vesicles"],"prefix":"10.1021","volume":"98","author":[{"given":"Shangbin","family":"Kao","sequence":"first","affiliation":[{"name":"Zhejiang Sci-Tech University , , , ,","place":["Hangzhou, Zhejiang, China, 310016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Tian","sequence":"additional","affiliation":[{"name":"Zhejiang Sci-Tech University , , , ,","place":["Hangzhou, Zhejiang, China, 310016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Sci-Tech University , , , ,","place":["Hangzhou, Zhejiang, China, 310016"]},{"name":"Hangzhou Normal University , , , ,","place":["Hangzhou, Zhejiang, China, 311202"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Qin","sequence":"additional","affiliation":[{"name":"Zhejiang Sci-Tech University , , , ,","place":["Hangzhou, Zhejiang, China, 310016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Gu","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510080"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongming","family":"Fang","sequence":"additional","affiliation":[{"name":"Hangzhou Normal University , , , ,","place":["Hangzhou, Zhejiang, China, 311202"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Zhejiang Sci-Tech University , , , ,","place":["Hangzhou, Zhejiang, China, 310016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Huang","sequence":"additional","affiliation":[{"name":"Zhejiang Sci-Tech University , , , ,","place":["Hangzhou, Zhejiang, China, 310016"]},{"name":"the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine) , , , ,","place":["Hangzhou, Zhejiang, China, 310006"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"2026081809085988700_cit1","doi-asserted-by":"publisher","first-page":"2774","DOI":"10.1021\/acs.analchem.3c02068","article-title":"Serological Exosome\nMetabolic Biopsy of Hepatocellular Carcinoma via Designed Core-Shell\nNanoparticles","volume":"96","author":"Yang","year":"2024","journal-title":"Anal. Chem."},{"issue":"5","key":"2026081809085988700_cit2","doi-asserted-by":"publisher","DOI":"10.3390\/bios12050281","article-title":"Rapid Capturing and\nChemiluminescent Sensing of Programmed Death Ligand-1 Expressing Extracellular\nVesicles","volume":"12","author":"Khan","year":"2022","journal-title":"Biosensors"},{"issue":"1","key":"2026081809085988700_cit3","doi-asserted-by":"publisher","first-page":"237","DOI":"10.7150\/thno.21945","article-title":"Exosome Theranostics:\nBiology and Translational Medicine","volume":"8","author":"He","year":"2018","journal-title":"Theranostics"},{"issue":"7","key":"2026081809085988700_cit4","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070727","article-title":"Overview of Extracellular\nVesicles, Their Origin, Composition,\nPurpose, and Methods for Exosome Isolation and Analysis","volume":"8","author":"Doyle","year":"2019","journal-title":"Cells"},{"issue":"2","key":"2026081809085988700_cit5","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1042\/BST20253109","article-title":"Emerging liquid biopsy tools to analyse\ncancer biomarkers: electrochemical sensors and extracellular vesicle\nanalysis","volume":"54","author":"Devadoss","year":"2026","journal-title":"Biochem. Soc. Trans."},{"issue":"1","key":"2026081809085988700_cit6","doi-asserted-by":"publisher","DOI":"10.1186\/s40580-021-00263-w","article-title":"Nanotechnology\nin emerging liquid biopsy applications","volume":"8","author":"Kalogianni","year":"2021","journal-title":"Nano\nConvergence"},{"issue":"2","key":"2026081809085988700_cit7","doi-asserted-by":"publisher","DOI":"10.3390\/genes14020259","article-title":"Progress of\nEndogenous and Exogenous Nanoparticles for Cancer Therapy and Diagnostics","volume":"14","author":"Fujita","year":"2023","journal-title":"Genes"},{"key":"2026081809085988700_cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2026.345477","article-title":"A review of exosome\nisolation based on membrane protein recognition: Methods, performance,\nand challenges","volume":"1410","author":"Guo","year":"2026","journal-title":"Anal. Chim. 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Biosci."},{"issue":"8","key":"2026081809085988700_cit24","doi-asserted-by":"publisher","DOI":"10.3390\/toxins15080501","article-title":"Okadaic Acid Detection\nthrough a Rapid and\nSensitive Amplified Luminescent Proximity Homogeneous Assay","volume":"15","author":"Qin","year":"2023","journal-title":"Toxins"},{"issue":"3","key":"2026081809085988700_cit25","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1039\/D5AY01720C","article-title":"Establishment and application\nof a method for detecting tetracycline antibiotics in milk based on\nAlphaLICA technology","volume":"18","author":"Li","year":"2026","journal-title":"Anal Methods."},{"issue":"10","key":"2026081809085988700_cit26","doi-asserted-by":"publisher","first-page":"2019","DOI":"10.1039\/D5AY02160J","article-title":"AlphaLICA-based homogeneous immunoassay for\nhighly sensitive detection of streptomycin in infant formula","volume":"18","author":"Li","year":"2026","journal-title":"Anal Methods."},{"key":"2026081809085988700_cit27","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4591","article-title":"Ultra-sensitive liquid\nbiopsy of circulating extracellular vesicles using ExoScreen","volume":"5","author":"Yoshioka","year":"2014","journal-title":"Nat. Commun."},{"issue":"45","key":"2026081809085988700_cit28","doi-asserted-by":"publisher","first-page":"15200","DOI":"10.1021\/acs.analchem.1c03957","article-title":"Magnetic-Nanowaxberry-Based\nSimultaneous Detection of Exosome and Exosomal Proteins for the Intelligent\nDiagnosis of Cancer","volume":"93","author":"Ding","year":"2021","journal-title":"Anal. Chem."},{"issue":"24","key":"2026081809085988700_cit29","doi-asserted-by":"publisher","first-page":"14402","DOI":"10.1021\/acs.analchem.8b03959","article-title":"ExoAPP: Exosome-Oriented,\nAptamer Nanoprobe-Enabled Surface Proteins Profiling and Detection","volume":"90","author":"Jin","year":"2018","journal-title":"Anal. 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Vesicles"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c03310","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/32\/23806\/66361701\/acs.analchem.6c03310.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/32\/23806\/66361701\/acs.analchem.6c03310.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:30:29Z","timestamp":1787059829000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/32\/23806\/5238688\/Logic-Gated-Dual-Aptamer-Proximity-AlphaLISA-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":34,"journal-issue":{"issue":"32","published-online":{"date-parts":[[2026,8,3]]},"published-print":{"date-parts":[[2026,8,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c03310","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]}}}