{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T11:42:18Z","timestamp":1792496538337,"version":"4.5.0"},"reference-count":56,"publisher":"American Chemical Society (ACS)","issue":"41","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100005790","name":"Thammasat University","doi-asserted-by":"publisher","award":["TUPD30\/2568"],"award-info":[{"award-number":["TUPD30\/2568"]}],"id":[{"id":"10.13039\/501100005790","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The HLA-B*13:01 allele is a critical biomarker for dapsone- and cotrimoxazole-induced severe cutaneous adverse reactions (SCARs). Overcoming the limitations of centralized laboratory screening, we developed a portable electrochemical dual-sensing platform (EDP) for the simultaneous, ultrasensitive detection of HLA-B*13:01 alongside a TIMP-1 internal control. On screen-printed carbon electrodes (SPCEs), TIMP-1 is captured through robust biotin\u2013streptavidin interactions, while HLA-B*13:01 relies on sequence-specific ssDNA hybridization. Crucially, the system utilizes methylene blue (MB) to precisely modulate both electrostatic repulsion and physical steric hindrance, enhancing signal discrimination. The platform demonstrates exceptional limits of detection, reaching 0.992 pg and 65.5 fg when evaluated by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. Notably, accounting for genomic copy number, this corresponds to a practical detection limit of \u223c3 HLA-B gene copies (1 \u00d7 10\u20132 ng genomic DNA), several orders of magnitude below conventional gel electrophoresis. Validated against 100 blinded clinical samples, the EDP achieved 100% sensitivity, specificity, and accuracy using ROC-derived cut-offs. By delivering a highly scalable, portable post-PCR screening tool, this work provides a high-performance alternative to standard methods, effectively mitigating SCAR risks and advancing the global accessibility of precision medicine.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03379","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:02:07Z","timestamp":1790071327000},"page":"29565-29578","source":"Crossref","is-referenced-by-count":0,"title":["Electrochemical Dual-Sensing Platform for\n                    <i>HLA-B*13:01<\/i>\n                    Detection to Prevent Severe Cutaneous Adverse\nReactions (SCARs) Induced by Dapsone and Cotrimoxazole"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6102-8371","authenticated-orcid":true,"given":"Jirapat","family":"Attapong","sequence":"first","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]},{"name":"Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chollanot","family":"Kaset","sequence":"additional","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]},{"name":"Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nontaya","family":"Nakkam","sequence":"additional","affiliation":[{"name":"Khon Kaen University , , ,","place":["Khon Kaen, Thailand, 40002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wichittra","family":"Tassaneeyakul","sequence":"additional","affiliation":[{"name":"Khon Kaen University , , ,","place":["Khon Kaen, Thailand, 40002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wichayaporn","family":"Kamsong","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanpen","family":"Karuwan","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chonlaphat","family":"Sukasem","sequence":"additional","affiliation":[{"name":"Ramathibodi Hospital , , ,","place":["Bangkok, Thailand, 10400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirinart","family":"Chomean","sequence":"additional","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]},{"name":"Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"2026102006572037500_cit1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.661135","article-title":"HLA-B*13:01 Is a Predictive\nMarker of Dapsone-Induced Severe Cutaneous Adverse Reactions in Thai\nPatients","volume":"12","author":"Satapornpong","year":"2021","journal-title":"Front. 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