{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:10:59Z","timestamp":1790082659392,"version":"4.0.1"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200076"],"award-info":[{"award-number":["32200076"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272423"],"award-info":[{"award-number":["82272423"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302638"],"award-info":[{"award-number":["82302638"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372348"],"award-info":[{"award-number":["82372348"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2024ZD0532700"],"award-info":[{"award-number":["2024ZD0532700"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017689","name":"Guangdong Provincial People's Hospital","doi-asserted-by":"publisher","award":["KJ012021097"],"award-info":[{"award-number":["KJ012021097"]}],"id":[{"id":"10.13039\/501100017689","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key-Area Research and Development Program of Guangdong Province","award":["2023B0101200014"],"award-info":[{"award-number":["2023B0101200014"]}]},{"name":"Natural Science Fund of Guangdong Province for Distinguished Young Scholars","award":["2025B1515020071"],"award-info":[{"award-number":["2025B1515020071"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Real-time and highly sensitive monitoring of pathogens in environmental and host-derived samples is critical for controlling emerging and acute infectious diseases, yet remains technically challenging. Here, we report a multifunctional nanozyme-based immunochromatographic assay (ICA) that enables rapid, universal, and multiplex detection in different scenarios through visual readouts. We constructed a corona-structured magnetic nanozyme (CSMN) with a triple signal-amplification mechanism involving \u201cmagnetic enrichment-synergistic enhancement-multiple catalytic sites,\u201d enabling efficient enrichment of trace pathogens in complex matrices and generation of dual visual outputs (colorimetric and catalytically enhanced signals) on ICA. The CSMN-based nanozyme ICA requires only 2 min of catalysis to achieve a \u226592.65-fold increase in sensitivity and a 100-fold expansion of the detection range. Based on this platform, dual-mode detection strategies were developed for SARS-CoV-2 and monkeypox virus. The direct mode enables rapid screening within 12 min, with detection limits as low as 19.4 and 22.5 pg\/mL for the two targets, respectively. The enrichment mode enables ultrasensitive detection within 20 min, with detection ranges of 0.0012\u20131000 ng\/mL and 0.0026\u20131000 ng\/mL, respectively, while mitigating interference from the hook effect, making it suitable for detecting low-abundance targets in complex samples. The method demonstrated high accuracy and reliability across environmental and clinical samples, with a detection time approximately one-sixth that of qPCR and ELISA, indicating strong potential for infectious disease surveillance and outbreak control.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03394","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:01:44Z","timestamp":1789117304000},"page":"27431-27445","source":"Crossref","is-referenced-by-count":0,"title":["Corona-Structured\nMagnetic Nanozymes Enable Dual-Mode\nImmunochromatographic Analysis for Flexible and Ultrasensitive Detection\nof Emerging Viruses"],"prefix":"10.1021","volume":"98","author":[{"given":"Meirou","family":"Lu","sequence":"first","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxuan","family":"Li","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Jia","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Bengbu Medical University , , ,","place":["Bengbu, China, 233004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qudi","family":"Qiao","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9627-107X","authenticated-orcid":true,"given":"Chongwen","family":"Wang","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jierong","family":"Chen","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7764-3213","authenticated-orcid":true,"given":"Bing","family":"Gu","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"2026092208062134100_cit1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2504318122","article-title":"The potential impacts of human genetics on virus emergence","volume":"122","author":"Langlois","year":"2025","journal-title":"P. Natl. Acad. Sci. U.S.A."},{"key":"2026092208062134100_cit2","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1038\/s41579-026-01305-y","article-title":"Mpox emergence,\nepidemiology, biology, clinical features and control","volume":"24","author":"Ogoina","year":"2026","journal-title":"Nat. Rev. Microbiol."},{"key":"2026092208062134100_cit3","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2475","article-title":"Dynamics of viral disease outbreaks:\nA hundred years (1918\/19-2019\/20). in retrospect - Loses, lessons\nand emerging issues","volume":"33","author":"Nii-Trebi","year":"2023","journal-title":"Rev. Med. Virol."},{"key":"2026092208062134100_cit4","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.chom.2022.11.016","article-title":"Rethinking next-generation vaccines\nfor coronaviruses, influenzaviruses, and other respiratory viruses","volume":"31","author":"Morens","year":"2023","journal-title":"Cell Host Microbe"},{"key":"2026092208062134100_cit5","doi-asserted-by":"publisher","first-page":"2103646","DOI":"10.1002\/adma.202103646","article-title":"Advanced Point-of-Care\nTesting Technologies for Human Acute Respiratory Virus Detection","volume":"34","author":"Zhang","year":"2022","journal-title":"Adv. Mater."},{"key":"2026092208062134100_cit6","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202201085","article-title":"Emerging Biosensing\nTechnologies for the Diagnostics of Viral Infectious Diseases","volume":"34","author":"Kabay","year":"2022","journal-title":"Adv. Mater."},{"key":"2026092208062134100_cit7","doi-asserted-by":"publisher","first-page":"2346","DOI":"10.1021\/acsnano.3c10427","article-title":"Molecular Engineering and Confinement\nEffect Powered Ultrabright Nanoparticles for Improving Sensitivity\nof Lateral Flow Immunoassay","volume":"18","author":"Zhang","year":"2024","journal-title":"ACS Nano"},{"key":"2026092208062134100_cit8","doi-asserted-by":"publisher","first-page":"6253","DOI":"10.1021\/acssensors.5c02084","article-title":"Dual-Pathway Lateral\nFlow Assay for Rapid and Sensitive SARS-CoV-2\nRNA Detection via CRISPR\/Cas13a-Mediated SERS","volume":"10","author":"Joung","year":"2025","journal-title":"ACS Sens."},{"key":"2026092208062134100_cit9","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202416906","article-title":"Antibody-level\nBacteria Grabbing by \u201cMechanic Invasion\u201d of Bioinspired\nHedgehog Artificial Mesoporous Nanostructure for Hierarchical Dynamic\nIdentification and Light-Response Sterilization","volume":"37","author":"Liu","year":"2025","journal-title":"Adv. Mater."},{"key":"2026092208062134100_cit10","doi-asserted-by":"publisher","first-page":"13042","DOI":"10.1002\/anie.202103458","article-title":"Ratiometric\nFluorescent Lateral Flow Immunoassay for\nPoint-of-Care Testing of Acute Myocardial Infarction","volume":"60","author":"Wang","year":"2021","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026092208062134100_cit11","doi-asserted-by":"publisher","first-page":"3788","DOI":"10.1038\/s41596-020-0357-x","article-title":"Tutorial: design and fabrication of nanoparticle-based\nlateral-flow immunoassays","volume":"15","author":"Parolo","year":"2020","journal-title":"Nat. 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Mater."},{"key":"2026092208062134100_cit19","doi-asserted-by":"publisher","first-page":"118320","DOI":"10.1016\/j.bios.2025.118320","article-title":"Redox-mediated synthesis of Kiwi-like hybrid nanoparticles enabling\nmultimode lateral flow immunoassay for detection of cardiac troponin\nI","volume":"295","author":"Wang","year":"2026","journal-title":"Biosens. Bioelectron."},{"key":"2026092208062134100_cit20","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1021\/acs.nanolett.3c03118","article-title":"Bimetallic Nanozyme: A Credible Tag for In\nSitu-Catalyzed Reporter\nDeposition in the Lateral Flow Immunoassay for Ultrasensitive Cancer\nDiagnosis","volume":"24","author":"Meng","year":"2024","journal-title":"Nano Lett."},{"key":"2026092208062134100_cit21","doi-asserted-by":"publisher","first-page":"147057","DOI":"10.1016\/j.cej.2023.147057","article-title":"Capture antibody\nimitator MnO2 nanozyme-based\ndual-signal immunochromatographic assay for rapid detection of Salmonella\nenteritidis","volume":"477","author":"Deng","year":"2023","journal-title":"Chem. Eng. J."},{"issue":"31","key":"2026092208062134100_cit22","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202502574","article-title":"Mercaptophenylboronic Acid-Mediated\nNanozyme Immunochromatographic\nAssay for Simultaneous Detection of Respiratory Bacteria and Virus","volume":"12","author":"Yu","year":"2025","journal-title":"Adv. Sci."},{"key":"2026092208062134100_cit23","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.bioactmat.2025.11.043","article-title":"Fishnet-like 2D magnetic\nnanozyme probe enables ultrasensitive bedside immunodiagnosis of bone\ninfection pathogens","volume":"58","author":"Wang","year":"2026","journal-title":"Bioact. Mater."},{"key":"2026092208062134100_cit24","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202310869","article-title":"\u201cFour-In-One\u201d Multifunctional\nDandelion-Like Gold@platinum Nanoparticles-Driven Multimodal Lateral\nFlow Immunoassay","volume":"20","author":"Wang","year":"2024","journal-title":"Small"},{"key":"2026092208062134100_cit25","doi-asserted-by":"publisher","first-page":"145110","DOI":"10.1016\/j.cej.2023.145110","article-title":"Polydopamine-coated\ntwo-dimensional nanomaterials as high-affinity photothermal signal\ntag towards dual-signal detection of Salmonella typhimurium by lateral\nflow immunoassay","volume":"472","author":"Yang","year":"2023","journal-title":"Chem. Eng. J."},{"key":"2026092208062134100_cit26","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202500134","article-title":"Ligand-Driven Annular-Epitaxial Growth\nof CuS-Au Heterostructures\nas Trinity Plasmonic Nanozyme for Multimode Diagnosis of Pathogenic\nBacteria","volume":"12","author":"Lin","year":"2025","journal-title":"Adv. Sci."},{"key":"2026092208062134100_cit27","doi-asserted-by":"publisher","first-page":"129853","DOI":"10.1016\/j.jhazmat.2022.129853","article-title":"Fe-N-C single-atom\nnanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins\nusing lateral flow immunoassay","volume":"441","author":"Cai","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"2026092208062134100_cit28","doi-asserted-by":"publisher","first-page":"25865","DOI":"10.1021\/acsnano.4c09513","article-title":"3D Film-Like\nNanozyme with a Synergistic\nAmplification Effect for the Ultrasensitive Immunochromatographic\nDetection of Respiratory Viruses","volume":"18","author":"Wang","year":"2024","journal-title":"ACS Nano"},{"key":"2026092208062134100_cit29","doi-asserted-by":"publisher","first-page":"116044","DOI":"10.1016\/j.bios.2024.116044","article-title":"Rapid and\nultra-sensitive lateral flow assay for pathogens based on multivalent\naptamer and magnetic nanozyme","volume":"250","author":"Li","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"2026092208062134100_cit30","doi-asserted-by":"publisher","first-page":"23982","DOI":"10.1021\/acs.analchem.5c03762","article-title":"Rambutan-Like Magnetic Nanozyme Modified with Wheat\nGerm Agglutinin Enhances Lateral Flow Immunoassay for Ultrasensitive\nand Broad-Spectrum Detection of Respiratory Microorganisms","volume":"97","author":"Liu","year":"2025","journal-title":"Anal. Chem."},{"key":"2026092208062134100_cit31","doi-asserted-by":"publisher","first-page":"19495","DOI":"10.1021\/acsami.9b03920","article-title":"Magnetic SERS\nStrip for Sensitive and Simultaneous Detection of Respiratory Viruses","volume":"11","author":"Wang","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026092208062134100_cit32","doi-asserted-by":"publisher","first-page":"13723","DOI":"10.1021\/acsnano.4c14869","article-title":"Ultrasmall PtIr Bimetallic\nNanozyme Treats Myocardial Infarction via Ischemic\/Inflammatory Cardiac\nMicroenvironment Remodeling","volume":"19","author":"Gong","year":"2025","journal-title":"ACS Nano"},{"issue":"11","key":"2026092208062134100_cit33","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1007\/s00604-025-07614-y","article-title":"Synergizing\nnanozyme-catalyzed signal\namplification with magnetic separation for sensitive dual-mode Listeria\nmonocytogenes detection","volume":"192","author":"Wang","year":"2025","journal-title":"Mikrochim. Acta"},{"key":"2026092208062134100_cit34","doi-asserted-by":"publisher","first-page":"4844","DOI":"10.1007\/s12274-024-6454-3","article-title":"Pt nanodendrites with a PtIr alloy\nsurface structure exhibit excellent stability toward acidic hydrogen\nevolution reaction","volume":"17","author":"Liu","year":"2024","journal-title":"Nano Res."},{"key":"2026092208062134100_cit35","doi-asserted-by":"publisher","first-page":"20402","DOI":"10.1021\/acsnano.3c06833","article-title":"Design and Mechanism\nInsight of Monodispersed AuCuPt Alloy Nanozyme\nwith Antitumor Activity","volume":"17","author":"Liu","year":"2023","journal-title":"ACS Nano"},{"key":"2026092208062134100_cit36","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202505712","article-title":"Electron Transfer-Tailored\nD-Band Center to Boost Nanozyme Catalysis for Interpretable Machine\nLearning-Empowered Intelligent Biosensing","volume":"12","author":"Li","year":"2025","journal-title":"Adv.\nSci."},{"key":"2026092208062134100_cit37","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1038\/s41467-023-44463-w","article-title":"A natural biogenic nanozyme for scavenging superoxide radicals","volume":"15","author":"Ma","year":"2024","journal-title":"Nat. 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Chem."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c03394","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/37\/27431\/68480407\/acs.analchem.6c03394.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/37\/27431\/68480407\/acs.analchem.6c03394.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:29:11Z","timestamp":1790080151000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/37\/27431\/5428171\/Corona-Structured-Magnetic-Nanozymes-Enable-Dual"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":38,"journal-issue":{"issue":"37","published-online":{"date-parts":[[2026,9,11]]},"published-print":{"date-parts":[[2026,9,22]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c03394","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}