{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:44:15Z","timestamp":1792784655135,"version":"4.5.0"},"reference-count":47,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2023GXNSFAA026044"],"award-info":[{"award-number":["2023GXNSFAA026044"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["32360245"],"award-info":[{"award-number":["32360245"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Highly sensitive quantification of biomarkers is essential for disease diagnosis, treatment monitoring, and prognosis evaluation. Recently, nanomaterial-based immunosensors have attracted increasing attention due to their high sensitivity. However, the preparation of nanomaterials is often complex, costly, and environmentally unfriendly. Here, we employed Escherichia coli O157:H7 as a nanoreporting probe, leveraging its dyeable genomic DNA to achieve direct signal amplification of target biomarkers. A multifunctional hairpin-shaped aptamer was designed to convert the target into measurable fluorescence signals from E. coli O157:H7 through bispecific binding and allosteric effects of the aptamer. Under optimized conditions, the E. coli O157:H7-based biosensor could detect 0.05 pg\/mL prostate-specific antigen (PSA), with a linear relationship ranging from 0.1 to 50 pg\/mL (correlation coefficient R2 = 0.988). The method also demonstrated excellent specificity and recovery rates (93\u2013107%), confirming its applicability for real-sample detection. A small-scale clinical study illustrated that the serum PSA levels were significantly elevated in patients with polycystic ovary syndrome compared to healthy controls. Hence, low-abundance PSA analysis may expand the utility of PSA in female-related diseases.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03478","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:01:56Z","timestamp":1788516116000},"page":"26756-26766","source":"Crossref","is-referenced-by-count":1,"title":["Sensitive Detection\nof Biomarker Using Multifunctional\nAptamer-Modified Fluorescent\n                    <i>E. coli<\/i>\n                    O157:H7"],"prefix":"10.1021","volume":"98","author":[{"given":"Zhao","family":"Li","sequence":"first","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zibo","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanyu","family":"Cui","sequence":"additional","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Cao","sequence":"additional","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1594-478X","authenticated-orcid":true,"given":"Shengbin","family":"He","sequence":"additional","affiliation":[{"name":"Guangxi Medical University , , , ,","place":["Nanning, Guangxi, P. R. China, 530021"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"2026091511072568600_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.150233","article-title":"Laser-induced\ngraphene (LIG)-based electrochemical microfluidic chip for simultaneous\nanalysis of multiplex microRNAs","volume":"486","author":"Liu","year":"2024","journal-title":"Chem. Eng.\nJ."},{"issue":"4","key":"2026091511072568600_cit2","doi-asserted-by":"publisher","first-page":"2176","DOI":"10.1021\/acs.analchem.4c05181","article-title":"CRISPR-based homogeneous\nelectrochemical strategy for\nnear-zero background detection of breast cancer extracellular vesicles\nvia fluidity-enhanced magnetic capture nanoprobe","volume":"97","author":"Yang","year":"2025","journal-title":"Anal. Chem."},{"key":"2026091511072568600_cit3","doi-asserted-by":"publisher","first-page":"1852","DOI":"10.1038\/s41591-026-04303-y","article-title":"A deep joint-learning\nproteomics model for diagnosis of six conditions\nassociated with dementia","volume":"32","author":"An","year":"2026","journal-title":"Nat. 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Macromol."},{"issue":"20","key":"2026091511072568600_cit9","doi-asserted-by":"publisher","first-page":"6541","DOI":"10.1039\/D0AN00585A","article-title":"Assembly of \u2033carrier\nfree\u2033 enzymatic nano-reporters\nfor improved ELISA","volume":"145","author":"Sun","year":"2020","journal-title":"Analyst"},{"issue":"9","key":"2026091511072568600_cit10","doi-asserted-by":"publisher","first-page":"5171","DOI":"10.1039\/D4NR04510F","article-title":"A high\nsensitivity prostate-specific antigen SERS detection platform based\non laser resonance nanoparticles","volume":"17","author":"Fu","year":"2025","journal-title":"Nanoscale"},{"issue":"1","key":"2026091511072568600_cit11","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-025-04092-3","article-title":"Discovery and validation\nof RAB3B as a diagnostic biomarker for prostate cancer with serum\nPSA below 10 ng\/mL based on a multi-omics study","volume":"26","author":"Zhao","year":"2026","journal-title":"Cancer Cell Int."},{"issue":"11","key":"2026091511072568600_cit12","doi-asserted-by":"publisher","first-page":"5911","DOI":"10.1093\/rheumatology\/keaf311","article-title":"Sex differences in\nserum proteomic profiles in psoriatic arthritis","volume":"64","author":"Dang","year":"2025","journal-title":"Rheumatology"},{"issue":"4","key":"2026091511072568600_cit13","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1111\/bju.16825","article-title":"Systematic\nreview of PSA reference intervals in the gender diverse population\nwith prostates","volume":"136","author":"Hall","year":"2025","journal-title":"BJU Int."},{"key":"2026091511072568600_cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2025.129110","article-title":"Toward clinical\ntranslation of eLFIA:\nanalytical tuning and serum application for PSA detection","volume":"299","author":"Miglione","year":"2026","journal-title":"Talanta"},{"issue":"2","key":"2026091511072568600_cit15","doi-asserted-by":"publisher","DOI":"10.1016\/j.soncn.2026.152167","article-title":"From serum\nmarkers to liquid biopsy: precision monitoring in breast, ovarian,\npancreatic, and prostate cancers","volume":"42","author":"Connors","year":"2026","journal-title":"Semin. Oncol.\nNurs."},{"key":"2026091511072568600_cit16","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.124742","article-title":"No-cost ballpoint\npen dispenser for lateral flow assays","volume":"263","author":"He","year":"2023","journal-title":"Talanta"},{"key":"2026091511072568600_cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2019.104223","article-title":"An electrochemical\nimmunosensor for CEA detection based\non Au-Ag\/rGO@PDA nanocomposites as integrated double signal amplification\nstrategy","volume":"151","author":"Yang","year":"2019","journal-title":"Microchem. J."},{"key":"2026091511072568600_cit18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117710","article-title":"Improved serum biomarker\ndetection via self-clickable\nCu2O nanoparticle-coated Ag slides with oriented and stable antibody\npresentation on antifouling surfaces","volume":"287","author":"Kuo","year":"2025","journal-title":"Biosens.\nBioelectron."},{"issue":"5","key":"2026091511072568600_cit19","doi-asserted-by":"publisher","first-page":"3959","DOI":"10.1021\/acs.analchem.5c06257","article-title":"Highly sensitive Zr-MOF-amplified electrochemical immunosensor of\nprostate-specific antigen with MWCNTs\/AuNPs\/MnO nanocomposites","volume":"98","author":"Gheybalizadeh","year":"2026","journal-title":"Anal. Chem."},{"issue":"48","key":"2026091511072568600_cit20","doi-asserted-by":"publisher","first-page":"17834","DOI":"10.1021\/acs.analchem.3c03914","article-title":"Integrating reliable\nPt-S bond-mediated 3D DNA nanomachine with magnetic\nseparation in a homogeneous electrochemical strategy for exosomal\nmicroRNA detection with low background and high sensitivity","volume":"95","author":"Yang","year":"2023","journal-title":"Anal. Chem."},{"issue":"32","key":"2026091511072568600_cit21","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202423106","article-title":"Laser-induced nanozyme\nbiofuel cell-based self-powered patch for\naccelerating diabetic wound healing with real-time monitoring","volume":"35","author":"Gu","year":"2025","journal-title":"Adv. Funct. 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Bioelectron."},{"issue":"3","key":"2026091511072568600_cit27","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1039\/D2AY01844F","article-title":"Bridge-DNA synthesis triggered by\nan allosteric aptamer for the colorimetric\ndetection of pathogenic bacteria","volume":"15","author":"Wang","year":"2023","journal-title":"Anal. Methods"},{"key":"2026091511072568600_cit28","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2026.03.013","article-title":"Multidimensionally\nengineered Escherichia coli for efficient\ngadusol biosynthesis with high-throughput quantitative analysis","author":"Zhang","year":"2026","journal-title":"Trends Biotechnol."},{"key":"2026091511072568600_cit29","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.foodhyd.2014.10.003","article-title":"Cross-linking of gelatin by chlorine\ndioxide steam","volume":"45","author":"He","year":"2015","journal-title":"Food Hydrocolloids"},{"issue":"3","key":"2026091511072568600_cit30","doi-asserted-by":"publisher","DOI":"10.1088\/2050-6120\/abf81e","article-title":"Using a safe\nand effective fixative to improve the\nimmunofluorescence staining of bacteria","volume":"9","author":"Sun","year":"2021","journal-title":"Methods\nAppl. Fluoresc."},{"key":"2026091511072568600_cit31","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.111485","article-title":"Quantifying\nthe viability of lactic acid bacteria using ratiometric fluorescence\nassays","volume":"206","author":"Chen","year":"2024","journal-title":"Microchem. J."},{"key":"2026091511072568600_cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2025.343607","article-title":"Biotinylation-based\nlateral flow assays for pathogenic and total\nbacteria detection","volume":"1338","author":"Cao","year":"2025","journal-title":"Anal. Chim. Acta"},{"key":"2026091511072568600_cit33","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2023.115384","article-title":"Comparison of plasma-activated\nwater and free chlorine in disinfecting &lt; i&gt; Escherichia coli O157:H7 and Salmonella Typhimurium-inoculated\nblueberry, cherry tomato, fresh-cut lettuce, and baby spinach","volume":"187","author":"Wang","year":"2023","journal-title":"LWT--Food Sci. Technol."},{"issue":"3","key":"2026091511072568600_cit34","doi-asserted-by":"publisher","first-page":"5937","DOI":"10.1021\/acsomega.9b00033","article-title":"Application of chlorine dioxide in\ncell surface modification to enhance Its mechanical stability and\nmetal Ion adsorption","volume":"4","author":"Li","year":"2019","journal-title":"ACS Omega"},{"key":"2026091511072568600_cit35","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.125206","article-title":"Transformation\nof proteins into reproductive DNA templates for sensitive quantification\nof PSA","volume":"267","author":"Li","year":"2024","journal-title":"Talanta"},{"key":"2026091511072568600_cit36","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.137240","article-title":"Sensitive detection of aflatoxin\nB1 in foods by aptasensing-based\nqPCR","volume":"432","author":"Sun","year":"2024","journal-title":"Food Chem."},{"key":"2026091511072568600_cit37","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2024.101886","article-title":"Real-time\nfluorescence growth curves for viable bacteria quantification in foods","volume":"24","author":"Chen","year":"2024","journal-title":"Food Chem.: X"},{"key":"2026091511072568600_cit38","doi-asserted-by":"publisher","first-page":"11018","DOI":"10.1021\/acs.analchem.4c01737","article-title":"Rapid and sensitive quantification of bacterial viability using ratiometric\nfluorescence sensing","volume":"96","author":"He","year":"2024","journal-title":"Anal. Chem."},{"key":"2026091511072568600_cit39","doi-asserted-by":"publisher","DOI":"10.1016\/j.snr.2025.100405","article-title":"Thermally driven death-melting\ncurve\nanalysis for rapid and straightforward identification of bacteria","volume":"10","author":"Chen","year":"2025","journal-title":"Sens. Actuators Rep."},{"issue":"13","key":"2026091511072568600_cit40","doi-asserted-by":"publisher","first-page":"10151","DOI":"10.1021\/acs.analchem.6c00834","article-title":"Rapid identification\nof yeasts in foods based on thermal-scanning fluorescence spectroscopy","volume":"98","author":"Chen","year":"2026","journal-title":"Anal. Chem."},{"issue":"4","key":"2026091511072568600_cit41","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.1016\/j.bios.2010.07.057","article-title":"Selection of DNA aptamer against\nprostate specific antigen using a genetic algorithm and application\nto sensing","volume":"26","author":"Savory","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"2026091511072568600_cit42","unstructured":"Bruno, J.; Chanpong, J.\n          Methods of Producing\nCompetitive Aptamer fret Reagents and Assays. U.S.\nPatent US2009\/0186342A1."},{"key":"2026091511072568600_cit43","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2019.127577","article-title":"Ultrasensitive\nbiosensing pathogenic bacteria by combining aptamer-induced catalysed\nhairpin assembly circle amplification with microchip electrophoresis","volume":"306","author":"Luo","year":"2020","journal-title":"Sens. Actuators, B"},{"issue":"11","key":"2026091511072568600_cit44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048999","article-title":"An aptamer-based biosensor for colorimetric\ndetection of Escherichia coli O157:\nH7","volume":"7","author":"Wu","year":"2012","journal-title":"PLoS One"},{"issue":"13","key":"2026091511072568600_cit45","doi-asserted-by":"publisher","first-page":"5695","DOI":"10.1021\/acs.analchem.2c05655","article-title":"Highly efficient signal\non\/off electrochemiluminescence\ngel aptasensor based on a controlled release strategy for the sensitive\ndetection of prostate specific antigen","volume":"95","author":"Zhao","year":"2023","journal-title":"Anal.\nChem."},{"issue":"14","key":"2026091511072568600_cit46","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202409966","article-title":"Agarose gel-coated\nnanochannel biosensor for detection of prostate-specific antigen in\nunprocessed whole blood samples","volume":"21","author":"Zhao","year":"2025","journal-title":"Small"},{"issue":"11","key":"2026091511072568600_cit47","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade0997","article-title":"Biointerface\nmineralization generates ultraresistant gut microbes as oral biotherapeutics","volume":"9","author":"Geng","year":"2023","journal-title":"Sci. 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