{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T11:47:04Z","timestamp":1792842424280,"version":"4.5.0"},"reference-count":40,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K01535"],"award-info":[{"award-number":["JP24K01535"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K21817"],"award-info":[{"award-number":["JP24K21817"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJAP2315"],"award-info":[{"award-number":["JPMJAP2315"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJSF2504"],"award-info":[{"award-number":["JPMJSF2504"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020964","name":"Fusion Oriented REsearch for disruptive Science and Technology","doi-asserted-by":"publisher","award":["JPMJFR232H"],"award-info":[{"award-number":["JPMJFR232H"]}],"id":[{"id":"10.13039\/501100020964","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004721","name":"University of Tokyo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004721","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Metal\u2013organic frameworks (MOFs) offer structurally precise and chemically programmable pore environments for chromatographic separations, yet their implementation as high-performance liquid chromatography (HPLC) stationary phases remains limited by insufficient control over particle morphology and mass transport. Here, we report a transport-focused evaluation of the mesoporous Zr-based MOF NU-1000 as an HPLC stationary phase. Micrometer-sized NU-1000 particles were prepared by modulator-assisted synthesis, and pore accessibility was systematically varied by comparing a benzoate-coordinated framework with its acid-treated, pore-open analogue. Reduced van Deemter and Pe\u0301clet-number analyses reveal that the pore-open NU-1000 phase provides substantially improved column kinetics, reaching a minimum reduced plate height of hmin = 4.68 and plate numbers up to N = 3334 on a 50 mm column. Under matched-retention conditions, NU-1000 outperforms a self-packed C18 reference and approaches the performance of a commercially packed C18 benchmark, despite its rod-shaped particle morphology. The practical resolving power of NU-1000 is further demonstrated by gradient separation of PEG 400, where discrete polymer homologues are resolved with monomer-unit precision and a peak capacity of 213.7. These results establish pore accessibility and particle size as key design parameters for MOF-packed HPLC columns and demonstrate that bare mesoporous MOF particles can directly deliver high packed-bed efficiencies exceeding 60,000 plates m\u20131, without relying on core\u2013shell architectures, hierarchical composites, or capillary-column formats.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03518","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:01:59Z","timestamp":1789484519000},"page":"28249-28258","source":"Crossref","is-referenced-by-count":1,"title":["Mesoporous\nMOF NU-1000 as a High-Efficiency HPLC Stationary\nPhase: Transport-Controlled Design and Quantitative Evaluation"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5245-9089","authenticated-orcid":true,"given":"Malvina","family":"Supper","sequence":"first","affiliation":[{"name":"The University of Tokyo , , 7-3-1 Hongo, Bunkyo-ku , ,","place":["Tokyo, Japan, 113-8656"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1357-3196","authenticated-orcid":true,"given":"Takashi","family":"Uemura","sequence":"additional","affiliation":[{"name":"The University of Tokyo , , 7-3-1 Hongo, Bunkyo-ku , ,","place":["Tokyo, Japan, 113-8656"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4145-0046","authenticated-orcid":true,"given":"Nobuhiko","family":"Hosono","sequence":"additional","affiliation":[{"name":"The University of Tokyo , , 7-3-1 Hongo, Bunkyo-ku , ,","place":["Tokyo, Japan, 113-8656"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"2026092909102975800_cit1","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1021\/ar2002599","article-title":"Metal-Organic Frameworks\nfor Analytical Chemistry: From Sample Collection to Chromatographic\nSeparation","volume":"45","author":"Gu","year":"2012","journal-title":"Acc. Chem. Res."},{"key":"2026092909102975800_cit2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.chroma.2017.10.065","article-title":"Metal-organic frameworks\nas stationary phase for application in chromatographic\nseparation","volume":"1530","author":"Zhang","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"2026092909102975800_cit3","doi-asserted-by":"publisher","first-page":"1230444","DOI":"10.1126\/science.1230444","article-title":"The Chemistry and\nApplications of Metal-Organic Frameworks","volume":"341","author":"Furukawa","year":"2013","journal-title":"Science"},{"key":"2026092909102975800_cit4","doi-asserted-by":"publisher","first-page":"969","DOI":"10.6023\/A16090482","article-title":"Application\nof Metal-Organic Frameworks in Chromatographic\nSeparation","volume":"74","author":"Li","year":"2016","journal-title":"Acta Chim. Sinica"},{"key":"2026092909102975800_cit5","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1002\/anie.200906560","article-title":"Metal\u2013Organic\nFramework MIL101 for High-Resolution\nGas Chromatographic Separation of Xylene Isomers and Ethylbenzene","volume":"49","author":"Gu","year":"2010","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026092909102975800_cit6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.chroma.2014.04.095","article-title":"Metal\u2013Organic Frameworks in\nChromatography","volume":"1348","author":"Yusuf","year":"2014","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit7","doi-asserted-by":"publisher","first-page":"214364","DOI":"10.1016\/j.ccr.2021.214364","article-title":"Metal-organic\nframeworks as stationary phases for chromatography and solid phase\nextraction: A review","volume":"455","author":"Kotova","year":"2022","journal-title":"Coord. Chem. Rev."},{"key":"2026092909102975800_cit8","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1039\/D0AY02310H","article-title":"Regulating metal\u2013organic\nframeworks as stationary phases and absorbents for analytical separations","volume":"13","author":"Meng","year":"2021","journal-title":"Anal. Methods"},{"key":"2026092909102975800_cit9","doi-asserted-by":"publisher","first-page":"102864","DOI":"10.1016\/j.cis.2023.102864","article-title":"Morphology control through the synthesis of metal-organic\nframeworks","volume":"314","author":"\u0141uczak","year":"2023","journal-title":"Advanced Colloid and Interface Science"},{"key":"2026092909102975800_cit10","doi-asserted-by":"publisher","first-page":"466481","DOI":"10.1016\/j.chroma.2025.466481","article-title":"Mechanistic insights\ninto band broadening in MOF-based\nliquid chromatography: Role of sub-nanopore diffusion and packing\nheterogeneity","volume":"1765","author":"Torimoto","year":"2026","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit11","first-page":"115874","article-title":"Metal-Organic\nFramework-Based Stationary\nPhases for Liquid Chromatography","volume":"126","author":"Liu","year":"2020","journal-title":"Trends Analyt.\nChem."},{"key":"2026092909102975800_cit12","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0021-9673(98)00497-X","article-title":"Band dispersion\nin chromatography \u2013 a new view of A-term dispersion","volume":"831","author":"Knox","year":"1999","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit13","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.chroma.2011.04.058","article-title":"Mass transfer kinetics, band broadening\nand column\nefficiency","volume":"1221","author":"Gritti","year":"2012","journal-title":"J. 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Commun."},{"key":"2026092909102975800_cit17","doi-asserted-by":"publisher","first-page":"13690","DOI":"10.1039\/D4CC04902K","article-title":"Detecting\nsingle-point isomeric differences in polymer chains by MOF column\nchromatography","volume":"60","author":"Hosono","year":"2024","journal-title":"Chem. Commun."},{"key":"2026092909102975800_cit18","doi-asserted-by":"publisher","first-page":"2400535","DOI":"10.1002\/marc.202400535","article-title":"Isolation of Polyethylene\nGlycol with Larger Molecular Weights via Metal\u2013Organic Frameworks","volume":"45","author":"Huang","year":"2024","journal-title":"Macromol. Rapid Commun."},{"key":"2026092909102975800_cit19","doi-asserted-by":"publisher","first-page":"11830","DOI":"10.1002\/anie.202102794","article-title":"Metal\u2013Organic\nFrameworks for Practical Separation of Cyclic and Linear Polymers","volume":"60","author":"Sawayama","year":"2021","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026092909102975800_cit20","doi-asserted-by":"publisher","first-page":"15655","DOI":"10.1021\/acs.analchem.0c04074","article-title":"Nanoscale\nHierarchically Micro- and Mesoporous Metal\u2013Organic Frameworks\nfor High-Resolution and High-Efficiency Capillary Electrochromatographic\nSeparation","volume":"92","author":"Ji","year":"2020","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit21","doi-asserted-by":"publisher","first-page":"16720","DOI":"10.1021\/acs.analchem.2c03185","article-title":"Homomesoporous\nMetal\u2013Organic Framework for High-Performance Electrochromatographic\nSeparation","volume":"94","author":"Gui","year":"2022","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit22","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1093\/nsr\/nwz170","article-title":"Hierarchically\nporous metal\u2013organic frameworks: synthetic strategies and applications","volume":"7","author":"Feng","year":"2020","journal-title":"National Science Review"},{"key":"2026092909102975800_cit23","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.chroma.2014.06.029","article-title":"Particle size distribution and column\nefficiency. An ongoing debate revived with 1.9\u03bcm Titan-C18 particles","volume":"1355","author":"Gritti","year":"2014","journal-title":"Journal of Chromatography\nA"},{"key":"2026092909102975800_cit24","doi-asserted-by":"publisher","first-page":"14251","DOI":"10.1021\/acs.analchem.2c02575","article-title":"Enhancing Separation Abilities of\n\u201cLow-Performance\u201d MOF Stationary Phases through Size\nControl","volume":"94","author":"Meng","year":"2022","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit25","doi-asserted-by":"publisher","first-page":"13975","DOI":"10.1021\/acs.analchem.6c00619","article-title":"Toward High\nPerformance Liquid Chromatography with Hierarchically\nPorous Metal Organic Framework Spheres (HP-MOFS)","volume":"98","author":"Li","year":"2026","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit26","doi-asserted-by":"publisher","first-page":"18067","DOI":"10.1021\/acs.analchem.2c04592","article-title":"Fluoro-Functionalized\nSpherical Covalent Organic Frameworks as a Liquid Chromatographic\nStationary Phase for the High-Resolution Separation of Organic Halides","volume":"94","author":"Zheng","year":"2022","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit27","doi-asserted-by":"publisher","first-page":"11815","DOI":"10.1021\/acs.analchem.5c01497","article-title":"Capsule-Type Single-Crystal Covalent\nOrganic Frameworks as an Advanced Chromatographic Stationary Phase\nfor Efficient Separation of Isomers","volume":"97","author":"Wu","year":"2025","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit28","doi-asserted-by":"publisher","first-page":"15198","DOI":"10.1021\/acs.analchem.6c01557","article-title":"Hydroxyl-Functionalized\nAcicular Single-Crystalline Covalent Organic Frameworks as Liquid\nChromatographic Stationary Phases for the High-Performance Separation\nof Isomers","volume":"98","author":"Xue","year":"2026","journal-title":"Anal. Chem."},{"key":"2026092909102975800_cit29","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1038\/nprot.2016.001","article-title":"Scalable synthesis\nand post-modification of a mesoporous metal-organic framework called\nNU-1000","volume":"11","author":"Wang","year":"2016","journal-title":"Nat. Protoc."},{"key":"2026092909102975800_cit30","doi-asserted-by":"publisher","first-page":"5913","DOI":"10.1039\/C8CE00455B","article-title":"Revisiting the structural\nhomogeneity of NU-1000, a Zr-based metal\u2013organic framework","volume":"20","author":"Islamoglu","year":"2018","journal-title":"CrystEngComm"},{"key":"2026092909102975800_cit31","doi-asserted-by":"publisher","first-page":"10294","DOI":"10.1021\/ja4050828","article-title":"Vapor-Phase Metalation\nby Atomic Layer Deposition in a Metal\u2013Organic Framework","volume":"135","author":"Mondloch","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"2026092909102975800_cit32","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.1021\/acs.cgd.9b01590","article-title":"Size Control of the MOF NU-1000 through Manipulation\nof the Modulator\/Linker Competition","volume":"20","author":"Webber","year":"2020","journal-title":"Cryst.\nGrowth Des."},{"key":"2026092909102975800_cit33","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1039\/c3cc48562e","article-title":"Versatile functionalization\nof the NU-1000 platform by solvent-assisted ligand incorporation","volume":"50","author":"Deria","year":"2014","journal-title":"Chem. Commun."},{"key":"2026092909102975800_cit34","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1021\/acscentsci.1c01344","article-title":"Homogeneously Mixing Different Metal\u2013Organic\nFramework Structures in Single Nanocrystals through Forming Solid\nSolutions","volume":"8","author":"Xu","year":"2022","journal-title":"ACS Central Science"},{"key":"2026092909102975800_cit35","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.chroma.2004.10.008","article-title":"On the optimization of the bed porosity and the particle\nshape of ordered chromatographic separation media","volume":"1073","author":"De Smet","year":"2005","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit36","doi-asserted-by":"publisher","first-page":"466894","DOI":"10.1016\/j.chroma.2026.466894","article-title":"Computational\nstudy of the mass transfer\neffects in metal\u2013organic framework columns for liquid chromatography","volume":"1774","author":"Torimoto","year":"2026","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit37","doi-asserted-by":"publisher","first-page":"8167","DOI":"10.1016\/j.chroma.2010.10.075","article-title":"Mass transfer mechanism in liquid\nchromatography columns\npacked with shell particles: Would there be an optimum shell structure?","volume":"1217","author":"Gritti","year":"2010","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit38","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.jpba.2009.08.003","article-title":"Facts and\nmyths about columns packed\nwith sub-3 \u03bcm and sub-2 \u03bcm particles","volume":"51","author":"Fekete","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"key":"2026092909102975800_cit39","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.chroma.2015.01.047","article-title":"The quantitative impact\nof the mesopore size on the\nmass transfer mechanism of the new 1.9\u03bcm fully\nporous Titan-C18 particles. I: analysis of small molecules","volume":"1384","author":"Gritti","year":"2015","journal-title":"Journal of Chromatography A"},{"key":"2026092909102975800_cit40","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.chroma.2016.05.054","article-title":"Extensive\ndatabase of liquid phase diffusion coefficients of some frequently\nused test molecules in reversed-phase liquid chromatography and hydrophilic\ninteraction liquid chromatography","volume":"1455","author":"Song","year":"2016","journal-title":"Journal of\nChromatography A"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c03518","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/28249\/68516629\/acs.analchem.6c03518.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/28249\/68516629\/acs.analchem.6c03518.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:33:34Z","timestamp":1790688814000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/38\/28249\/5430306\/Mesoporous-MOF-NU-1000-as-a-High-Efficiency-HPLC"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":40,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,15]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c03518","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]}}}