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This fully automated \u201csample-in to result-out\u201d system integrates all reagents and consumables within a compact reagent cartridge and analyzer, enabling sensitive detection from only 200 \u03bcL of plasma within 25 min. The platform employs a streptavidin\u2013biotin amplification strategy and features precise pipetting, uniform temperature control, efficient washing, and low background signal, ensuring high accuracy and reproducibility. The platform exhibits enhanced analytical performance, with limits of detection (LoD) of 0.5 ng\/mL for TAT, 1.0 TU\/mL for TM, 0.06 \u03bcg\/mL for PIC, 0.75 ng\/mL for t-PAIC, and 5 ng\/mL for D-dimer. The repeatability coefficient of variation (CV) is below 8%, the within-laboratory CV is under 10%, bias remains within 8%, and linearity is demonstrated by an R2 value exceeding 0.99. A method comparison study conducted on 228 clinical samples against the reference system HISCL-5000 showed a strong correlation (R2 &amp;gt; 0.99) and agreement exceeding 96% within the Bland-Altman limits. Overall, this innovative multiplex immunoassay system offers a rapid, cost-effective, and accessible solution for comprehensive coagulation-fibrinolysis assessment, with significant potential to improve real-time clinical management of thrombotic and coagulopathic disorders, especially in emergency and point-of-care settings.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03533","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:03:01Z","timestamp":1785852181000},"page":"23314-23324","source":"Crossref","is-referenced-by-count":0,"title":["An Integrated\nCartridge-Based Multiplex Chemiluminescent\nImmunosensor Platform for the Simultaneous Quantification of Five\nCoagulation and Fibrinolysis Biomarkers"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8815-7429","authenticated-orcid":true,"given":"Meng","family":"Liu","sequence":"first","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]},{"name":"Jiangsu Baiming Biotechnology Co., Ltd. , ,","place":["Yancheng, China, 224000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8586-606X","authenticated-orcid":true,"given":"Chaolin","family":"Guo","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaipeng","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]},{"name":"Jiangsu Baiming Biotechnology Co., Ltd. , ,","place":["Yancheng, China, 224000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqi","family":"Nie","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Xie","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Li","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Zhejiang, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4815-6832","authenticated-orcid":true,"given":"Yankun","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Sun","sequence":"additional","affiliation":[{"name":"Henan University , , ,","place":["Kaifeng, China, 475004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Ma","sequence":"additional","affiliation":[{"name":"Wuxi Stomatological Hospital , , ,","place":["Wuxi, China, 214023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3985-7434","authenticated-orcid":true,"given":"Zhonghu","family":"Bai","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214126"]},{"name":"Jiangnan University , , ,","place":["Wuxi, People\u2019s Republic of China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"issue":"4","key":"2026081109083058100_cit1","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1160\/TH13-04-0345","article-title":"Simultaneous Measurement of Thrombin\nand Plasmin Generation to Assess the Interplay between Coagulation\nand Fibrinolysis","volume":"110","author":"Matsumoto","year":"2013","journal-title":"Thromb. 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