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In this study, two chiral compounds (TPA-PyrT@CSAD and TPA-PyrT@CSAL) were obtained using an anion replacement strategy, which shares the same fluorescent skeleton of TPA-PyrT and the chiral anion of camphor sulfonic salt (CAS) with opposite configurations as D or L. First, the chirality-dependent recognition of several bio-molecules was realized; in addition, these probes were verified with obvious discriminating ability to cancer cells or normal cells, demonstrating different mitochondria-imaging capability and dynamic cell endocytosis processes. Owing to its excellent sensing performance, TPA-PyrT@CSAD was successfully applied to monitor the concentration fluctuations of O2\u2022- in mitochondria. Finally, the in vivo biosafety was evaluated using zebrafish as a model.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03585","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:35:20Z","timestamp":1788438920000},"page":"26797-26807","source":"Crossref","is-referenced-by-count":0,"title":["Simple Way\nto Fabricate Chiral Fluorescent Molecules\nand Chirality-Dependent Biomolecule Recognition and Cell Imaging"],"prefix":"10.1021","volume":"98","author":[{"given":"Shuangyu","family":"Dong","sequence":"first","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongting","family":"Fan","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muheman","family":"Li","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanbao","family":"Wang","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huhu","family":"Wang","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haowen","family":"Huang","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxuan","family":"Qi","sequence":"additional","affiliation":[{"name":"Baoji University of Arts and Sciences , , ,","place":["Baoji, China, 721013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-tao","family":"Feng","sequence":"additional","affiliation":[{"name":"Baoji University of Arts and Sciences , , ,","place":["Baoji, China, 721013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4161-5373","authenticated-orcid":true,"given":"Dedai","family":"Lu","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9195-4472","authenticated-orcid":true,"given":"Ziqiang","family":"Lei","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2348-9585","authenticated-orcid":true,"given":"Hengchang","family":"Ma","sequence":"additional","affiliation":[{"name":"Northwest Normal University , , ,","place":["Lanzhou, China, 730070"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"issue":"1","key":"2026091511085562000_cit1","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1080\/10587250108028273","article-title":"Chiral\ninteractions and structures","volume":"358","author":"Kamien","year":"2001","journal-title":"Mol. 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