{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:42:10Z","timestamp":1792784530763,"version":"4.5.0"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100010896","name":"International Cooperation and Exchange Programme","doi-asserted-by":"publisher","award":["W2512055"],"award-info":[{"award-number":["W2512055"]}],"id":[{"id":"10.13039\/501100010896","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Advanced Research Capacity Building Program of Ningbo University","award":["GJPY2026024"],"award-info":[{"award-number":["GJPY2026024"]}],"id":[{"id":"https:\/\/ror.org\/03et85d35","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The precise analysis of chirality is crucial for elucidating their stereoselective effects. Ion mobility\u2013mass spectrometry (IM-MS) is a promising tool for chirality analysis, yet it is constrained by limited resolving power and the correlation between collision cross-section (CCS) values and accurate structures. In this work, a rapid method for recognizing matrine (MAT) and its enantiomer sophoridine (SPD) was established using IM-MS and theoretical modeling. With Natamycin (Nat) and Amphotericin B (Amp) as chiral selectors, binary noncovalent complexes were formed with target analytes to enhance stereostructural differences and enable separation. MAT and SPD were effectively separated via [MAT\/SPD+Nat+H]+ and [MAT\/SPD+Amp+H]+ complexes, with peak-to-peak resolutions (Rp\u2013p) of 1.12 and 3.46, yielding 12.2 and 37.6-folds higher than that of free-state enantiomers (0.092), respectively. Theoretical modeling and noncovalent interaction analysis revealed key interaction sites and force types, explaining the differences in mobility behavior at the molecular level. Surface electrostatic potential analysis reveals that smaller CCS values of the complex relate to concentrated negative potential and stronger surface polarity, while larger CCS values lead to dispersed negative potential and weaker polarity. Tandem mass spectrometry confirmed stability variations between isomeric complexes, with larger CCS differences correlating with more significant dissociation energy differences. Applied to practical samples, the method achieved a detection limit of 0.0011 \u03bcmol\u00b7L\u20131, recoveries \u226576.00% and relative standard deviations \u22644.62%, and the mechanistic conclusions derived from MAT\/SPD can be generalized to other matrine-type alkaloid enantiomers, thereby providing a robust and efficient universal solution for their rapid analysis.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03587","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:56:57Z","timestamp":1788429417000},"page":"26872-26882","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Recognition\nof Matrine Enantiomers Using Ion\nMobility\u2013Mass Spectrometry and Theoretical Modeling with Macrolide\nas Chiral Selectors"],"prefix":"10.1021","volume":"98","author":[{"given":"Huang","family":"Zhao","sequence":"first","affiliation":[{"name":"Ningbo First Hospital Affiliated to Ningbo University, Ningbo University , , 247 Renmin Road, Jiangbei District , , ,","place":["Ningbo, Zhejiang, China, 315010"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5207-8473","authenticated-orcid":true,"given":"Fangling","family":"Wu","sequence":"additional","affiliation":[{"name":"Ningbo University , , , ,","place":["Ningbo, Zhejiang, China, 315211"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningbo University , , , ,","place":["Ningbo, Zhejiang, China, 315211"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Zhu","sequence":"additional","affiliation":[{"name":"Ningbo First Hospital Affiliated to Ningbo University, Ningbo University , , 247 Renmin Road, Jiangbei District , , ,","place":["Ningbo, Zhejiang, China, 315010"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningbo First Hospital Affiliated to Ningbo University, Ningbo University , , 247 Renmin Road, Jiangbei District , , ,","place":["Ningbo, Zhejiang, China, 315010"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xishi","family":"Wu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , 1219 Zhongguan West Road , , ,","place":["Ningbo, Zhejiang, China, 315201"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan-Fan","family":"Ding","sequence":"additional","affiliation":[{"name":"Ningbo University , , , ,","place":["Ningbo, Zhejiang, China, 315211"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"issue":"1","key":"2026091511084952000_cit1","doi-asserted-by":"publisher","first-page":"16","DOI":"10.4103\/2229-516X.112233","article-title":"A review of drug isomerism and its\nsignificance","volume":"3","author":"Chhabra","year":"2013","journal-title":"Int. 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