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Methods for rigorously fitting spectra, increasing throughput, accounting for background signals, and deconvoluting overlapping emission lines continue to evolve. We show here that quantitative fits of XFM data obtained after removing a baseline, calculated by peak stripping, can be unexpectedly dependent upon acquisition dwell-time and spectral aggregation, leading to differences in apparent elemental content. Using mouse preimplantation embryos and ovarian follicles as model samples, we demonstrate how these variables influence quantitative comparisons between samples. We find that subtracting an empirically measured blank spectrum instead of a baseline provides quantitative XFM elemental mapping results that are independent of dwell time and spectral aggregation dependencies.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03597","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:02:14Z","timestamp":1789578134000},"page":"28272-28284","source":"Crossref","is-referenced-by-count":0,"title":["Blank Spectrum\nCorrection as a Robust Solution to\nArtifacts in Quantitative X-ray Fluorescence Mapping"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4641-7955","authenticated-orcid":true,"given":"Andrew M.","family":"Crawford","sequence":"first","affiliation":[{"name":"Michigan State University , , 567 Wilson Rd. , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Balough","sequence":"additional","affiliation":[{"name":"Northwestern University , , 303 E. Superior St. , , ,","place":["Chicago, Illinois, United States, 60611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Northwestern University , , 303 E. Superior St. , , ,","place":["Chicago, Illinois, United States, 60611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoling","family":"Jin","sequence":"additional","affiliation":[{"name":"Northwestern University , , 2145 Sheridan Rd. , , ,","place":["Evanston, Illinois, United States, 60208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith W.","family":"MacRenaris","sequence":"additional","affiliation":[{"name":"Michigan State University , , 567 Wilson Rd. , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth","family":"Garwin","sequence":"additional","affiliation":[{"name":"Michigan State University , , 567 Wilson Rd. , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa K.","family":"Woodruff","sequence":"additional","affiliation":[{"name":"Northwestern University , , 303 E. Superior St. , , ,","place":["Chicago, Illinois, United States, 60611"]},{"name":"Michigan State University , , 965 Wilson Rd. , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Northwestern University , , 2145 Sheridan Rd. , , ,","place":["Evanston, Illinois, United States, 60208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0314-1274","authenticated-orcid":true,"given":"James E.","family":"Penner-Hahn","sequence":"additional","affiliation":[{"name":"University of Michigan, 930 N. University Ave. , , , ,","place":["Ann Arbor, Michigan, United States, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas V.","family":"O\u2019Halloran","sequence":"additional","affiliation":[{"name":"Michigan State University , , 567 Wilson Rd. , , ,","place":["East Lansing, Michigan, United States, 48824"]},{"name":"Michigan State University , , 578 S. Shaw Ln , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"issue":"3","key":"2026092909101051200_cit1","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1017\/S1551929515000401","article-title":"The Bionanoprobe: Synchrotron-based\nHard X-ray Fluorescence Microscopy for 2D\/3D Trace Element Mapping","volume":"23","author":"Chen","year":"2015","journal-title":"Microsc. 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