{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:16:17Z","timestamp":1788218177051,"version":"build-2803163510"},"reference-count":50,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22407002"],"award-info":[{"award-number":["22407002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2023AH050551"],"award-info":[{"award-number":["2023AH050551"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2024AH050706"],"award-info":[{"award-number":["2024AH050706"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2024AH050714"],"award-info":[{"award-number":["2024AH050714"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Doctoral Start-up Foundation of Anhui Medical University","doi-asserted-by":"publisher","award":["0801034201"],"award-info":[{"award-number":["0801034201"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Doctoral Start-up Foundation of Anhui Medical University","doi-asserted-by":"publisher","award":["0801043201"],"award-info":[{"award-number":["0801043201"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2024YFC2707200"],"award-info":[{"award-number":["2024YFC2707200"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Assessment of pathogenic mitochondrial DNA (mtDNA) single-nucleotide variant (SNV) heteroplasmy is important for molecular diagnostics, yet rapid visual profiling remains analytically challenging because an assay must combine single-nucleotide allelic discrimination, mutant-fraction-associated readout, and suitable target access. Herein, we report VISTA (visual identification and stratification of targeted mtDNA alleles), a broad-PAM FnCas12a assay that rebalances trans-cleavage signal output and mutant\u2013wild-type discrimination for visual mtDNA SNV heteroplasmy analysis. VISTA uses unmodified FnCas12a with relaxed TTN PAM recognition and integrates crRNA spacer-length engineering with PEG8000\/acBSA reaction tuning to improve the practical signal\u2013discrimination balance without nuclease engineering. At the m.3243A&amp;gt;G model locus, spacer truncation enhanced mutant\u2013wild-type discrimination, while molecular-dynamics simulations identified spacer-dependent differences between matched and mismatched complexes at the crRNA\u2013DNA interface. The optimized assay resolved defined synthetic m.3243A&amp;gt;G heteroplasmy gradients by fluorescence imaging and was further adapted to lateral-flow detection. In locus-specific analyses of a deidentified collection of 74 peripheral-blood samples, fluorescence and lateral-flow readouts achieved ROC AUC values above 0.9 for mutant-allele classification after target-region amplification. Fluorescence supported heteroplasmy-associated profiling, whereas lateral flow provided a visual, semiquantitative readout for relative ranking based on the T\/C ratio rather than absolute heteroplasmy measurement. VISTA therefore provides an accessible dual-readout analytical strategy for visual detection and heteroplasmy-associated profiling by tuning the FnCas12a signal output and allelic discrimination.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04072","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T12:02:21Z","timestamp":1786708941000},"page":"24507-24520","source":"Crossref","is-referenced-by-count":0,"title":["Visual Detection\nand Stratification of Pathogenic\nmtDNA SNV Heteroplasmy by Balancing FnCas12a Signal Output and Allelic\nDiscrimination"],"prefix":"10.1021","volume":"98","author":[{"given":"ShaoYu","family":"Yang","sequence":"first","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Shen","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Zhikang Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University , , ,","place":["Hefei, China, 230022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LeYao","family":"Liu","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhen","family":"Han","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Chen","sequence":"additional","affiliation":[{"name":"University College London , , ,","place":["London, U.K., NW3 2PF"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HongYu","family":"Bao","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Ji","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University , , ,","place":["Hefei, China, 230022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pai","family":"Peng","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-6136","authenticated-orcid":true,"given":"Yi","family":"Du","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , , ,","place":["Hefei, Anhui, China, 230032"]},{"name":"The First Affiliated Hospital of Anhui Medical University , , ,","place":["Hefei, China, 230022"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"2026083118460292500_cit1","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/290457a0","article-title":"Sequence and organization\nof the human mitochondrial genome","volume":"290","author":"Anderson","year":"1981","journal-title":"Nature"},{"key":"2026083118460292500_cit2","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1038\/nrg1606","article-title":"Mitochondrial DNA mutations in human disease","volume":"6","author":"Taylor","year":"2005","journal-title":"Nat. Rev. Genet."},{"key":"2026083118460292500_cit3","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201949612","article-title":"Mitochondrial DNA\nheteroplasmy in disease and targeted\nnuclease-based therapeutic approaches","volume":"21","author":"Nissanka","year":"2020","journal-title":"EMBO\nRep."},{"key":"2026083118460292500_cit4","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201708262","article-title":"mtDNA heteroplasmy level and copy number indicate disease burden\nin m.3243A&gt;G mitochondrial disease","volume":"10","author":"Grady","year":"2018","journal-title":"EMBO\nMol.\nMed."},{"key":"2026083118460292500_cit5","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1038\/348651a0","article-title":"A mutation in the tRNA(Leu)(UUR)\ngene associated with the MELAS subgroup of mitochondrial encephalomyopathies","volume":"348","author":"Goto","year":"1990","journal-title":"Nature"},{"key":"2026083118460292500_cit6","first-page":"428","article-title":"A new mitochondrial\ndisease associated with mitochondrial DNA heteroplasmy","volume":"46","author":"Holt","year":"1990","journal-title":"Am. J. Hum. Genet."},{"key":"2026083118460292500_cit7","first-page":"629","article-title":"Prenatal diagnosis of mitochondrial DNA8993 T-G disease","volume":"50","author":"Harding","year":"1992","journal-title":"Am. J. Hum. Genet."},{"key":"2026083118460292500_cit8","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1086\/302488","article-title":"Genetic counseling\nand prenatal diagnosis for the mitochondrial DNA mutations at nucleotide\n8993","volume":"65","author":"White","year":"1999","journal-title":"Am. J. Hum. Genet."},{"key":"2026083118460292500_cit9","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1136\/jmedgenet-2012-101172","article-title":"Preimplantation genetic diagnosis in mitochondrial\nDNA disorders: challenge and success","volume":"50","author":"Smeets","year":"2013","journal-title":"J. Med.\nGenet."},{"key":"2026083118460292500_cit10","doi-asserted-by":"publisher","first-page":"115676","DOI":"10.1016\/j.bios.2023.115676","article-title":"RatioCRISPR:\nA ratiometric\nbiochip based on CRISPR\/Cas12a for automated and multiplexed detection\nof heteroplasmic SNPs in mitochondrial DNA","volume":"241","author":"Wu","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"2026083118460292500_cit11","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.ajhg.2010.07.014","article-title":"Detecting\nHeteroplasmy from High-Throughput\nSequencing of Complete Human Mitochondrial DNA Genomes","volume":"87","author":"Li","year":"2010","journal-title":"Am. J. Hum. Genet."},{"key":"2026083118460292500_cit12","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.mito.2019.10.006","article-title":"Sensitivity of mitochondrial\nDNA heteroplasmy detection using Next Generation Sequencing","volume":"50","author":"Gonza\u0301lez","year":"2020","journal-title":"Mitochondrion"},{"key":"2026083118460292500_cit13","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1016\/j.jmoldx.2021.03.002","article-title":"Current and\nNew Next-Generation Sequencing\nApproaches to Study Mitochondrial DNA","volume":"23","author":"Legati","year":"2021","journal-title":"J. Mol.\nDiagn."},{"key":"2026083118460292500_cit14","doi-asserted-by":"publisher","first-page":"102574","DOI":"10.1016\/j.jbc.2022.102574","article-title":"Precise and simultaneous\nquantification of mitochondrial DNA heteroplasmy and copy number by\ndigital PCR","volume":"298","author":"Shoop","year":"2022","journal-title":"J. Biol. Chem."},{"key":"2026083118460292500_cit15","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1093\/labmed\/lmad063","article-title":"Droplet digital polymerase chain reaction to measure heteroplasmic\nm.3243A&gt;G mitochondrial mutations","volume":"55","author":"Matsumoto","year":"2024","journal-title":"Lab. Med."},{"key":"2026083118460292500_cit16","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection\nwith CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"2026083118460292500_cit17","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic\nacid detection platform with Cas13,\nCas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"2026083118460292500_cit18","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes\nindiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"2026083118460292500_cit19","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/s41421-018-0028-z","article-title":"CRISPR-Cas12a-assisted\nnucleic acid detection","volume":"4","author":"Li","year":"2018","journal-title":"Cell Discov"},{"key":"2026083118460292500_cit20","doi-asserted-by":"publisher","first-page":"17399","DOI":"10.1002\/anie.201910772","article-title":"Exploring the Trans-Cleavage Activity\nof CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical\nBiosensor","volume":"58","author":"Dai","year":"2019","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026083118460292500_cit21","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1016\/j.cell.2015.09.038","article-title":"Cpf1 is a single RNA-guided\nendonuclease of a class 2 CRISPR-Cas system","volume":"163","author":"Zetsche","year":"2015","journal-title":"Cell"},{"key":"2026083118460292500_cit22","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/j.molcel.2017.06.035","article-title":"Structural Basis for\nthe Canonical and Non-canonical\nPAM Recognition by CRISPR-Cpf1","volume":"67","author":"Yamano","year":"2017","journal-title":"Mol. Cell"},{"key":"2026083118460292500_cit23","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1038\/s41467-026-68476-3","article-title":"Identification of thermotolerant\nnon-canonical PAMs\nfor robust one-pot CRISPR-Cas12a detection","volume":"17","author":"Tian","year":"2026","journal-title":"Nat. Commun."},{"key":"2026083118460292500_cit24","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1016\/j.molcel.2018.06.043","article-title":"Kinetic Basis for DNA\nTarget Specificity of CRISPR-Cas12a","volume":"71","author":"Strohkendl","year":"2018","journal-title":"Mol. Cell"},{"key":"2026083118460292500_cit25","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1016\/j.molcel.2018.11.021","article-title":"Mechanistic Insights\ninto the cis- and trans-Acting DNase Activities\nof Cas12a","volume":"73","author":"Swarts","year":"2019","journal-title":"Mol. Cell"},{"key":"2026083118460292500_cit26","doi-asserted-by":"publisher","first-page":"100912","DOI":"10.1016\/j.crmeth.2024.100912","article-title":"Synthetic mismatches enable specific\nCRISPR-Cas12a-based detection of genome-wide SNVs tracked by ARTEMIS","volume":"4","author":"Kohabir","year":"2024","journal-title":"Cell Rep. Methods"},{"key":"2026083118460292500_cit27","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1021\/acssensors.2c02495","article-title":"CRISPR-Cas12a Biosensor Array for Ultrasensitive\nDetection of Unamplified\nDNA with Single-Nucleotide Polymorphic Discrimination","volume":"8","author":"Weng","year":"2023","journal-title":"ACS Sens."},{"key":"2026083118460292500_cit28","doi-asserted-by":"publisher","first-page":"5538","DOI":"10.1074\/jbc.RA120.012933","article-title":"CRISPR-Cas12a has\nwidespread off-target and dsDNA-nicking effects","volume":"295","author":"Murugan","year":"2020","journal-title":"J. Biol. Chem."},{"key":"2026083118460292500_cit29","doi-asserted-by":"publisher","first-page":"11295","DOI":"10.1093\/nar\/gkx783","article-title":"A \u2018new\nlease of life\u2019: FnCpf1 possesses DNA cleavage activity for\ngenome editing in human cells","volume":"45","author":"Tu","year":"2017","journal-title":"Nucleic Acids\nRes."},{"key":"2026083118460292500_cit30","doi-asserted-by":"publisher","first-page":"gkaf988","DOI":"10.1093\/nar\/gkaf988","article-title":"CRISPR-Cas12a REC2\u2013Nuc\ninteractions drive target-strand\ncleavage and constrain trans cleavage","volume":"53","author":"Newman","year":"2025","journal-title":"Nucleic\nAcids Res."},{"key":"2026083118460292500_cit31","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1038\/nbt.2808","article-title":"Improving\nCRISPR-Cas nuclease specificity using truncated guide RNAs","volume":"32","author":"Fu","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"2026083118460292500_cit32","doi-asserted-by":"publisher","first-page":"100080","DOI":"10.1016\/j.cellin.2023.100080","article-title":"Cas12a-based\none-pot SNP detection with high accuracy","volume":"2","author":"Zhang","year":"2023","journal-title":"Cell\nInsight"},{"key":"2026083118460292500_cit33","doi-asserted-by":"publisher","first-page":"11293","DOI":"10.1021\/jacs.8b05309","article-title":"Direct Visualization\nof Single-Nucleotide Variation\nin mtDNA Using a CRISPR\/Cas9-Mediated Proximity Ligation Assay","volume":"140","author":"Zhang","year":"2018","journal-title":"J. Am. Chem. Soc."},{"key":"2026083118460292500_cit34","doi-asserted-by":"publisher","first-page":"15117","DOI":"10.1021\/acs.analchem.2c03338","article-title":"Detection\nand Discrimination of Single Nucleotide Polymorphisms by Quantification\nof CRISPR-Cas Catalytic Efficiency","volume":"94","author":"Blanluet","year":"2022","journal-title":"Anal. Chem."},{"key":"2026083118460292500_cit35","doi-asserted-by":"publisher","first-page":"8342","DOI":"10.1038\/s41467-024-52691-x","article-title":"Split crRNA with CRISPR-Cas12a\nenabling highly sensitive and multiplexed detection of RNA and DNA","volume":"15","author":"Chen","year":"2024","journal-title":"Nat. Commun."},{"key":"2026083118460292500_cit36","doi-asserted-by":"publisher","first-page":"5597","DOI":"10.1038\/s41467-025-60917-9","article-title":"Cas12a Cis-cleavage mediated lateral flow assay\nenables multiplex\nand ultra-specific nucleic acid detection","volume":"16","author":"Lin","year":"2025","journal-title":"Nat.\nCommun."},{"key":"2026083118460292500_cit37","doi-asserted-by":"publisher","first-page":"1485","DOI":"10.1038\/s41587-024-02426-6","article-title":"Amplifying\nmutational profiling of extracellular vesicle\nmRNA with SCOPE","volume":"43","author":"Song","year":"2025","journal-title":"Nat. Biotechnol."},{"key":"2026083118460292500_cit38","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-025-01597-0","article-title":"Digital\nCRISPR-based diagnostics for quantification of Candida auris and resistance\nmutations","author":"Rolando","year":"2026","journal-title":"Nat. Biomed. Eng."},{"key":"2026083118460292500_cit39","doi-asserted-by":"publisher","first-page":"2141","DOI":"10.1038\/s41551-025-01424-6","article-title":"LbuCas13a directly targets\nDNA and elicits strong trans-cleavage\nactivity","volume":"9","author":"Wu","year":"2025","journal-title":"Nat. Biomed. Eng."},{"key":"2026083118460292500_cit40","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-025-02868-6","article-title":"RNA structure modulates Cas13 activity and\nenables mismatch detection","author":"Larsen","year":"2025","journal-title":"Nat. Biotechnol."},{"key":"2026083118460292500_cit41","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1126\/science.aas8836","article-title":"Field-deployable\nviral\ndiagnostics using CRISPR-Cas13","volume":"360","author":"Myhrvold","year":"2018","journal-title":"Science"},{"key":"2026083118460292500_cit42","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.412","article-title":"Next generation\nsequencing to characterize mitochondrial genomic DNA heteroplasmy","volume":"2","author":"Huang","year":"2011","journal-title":"Curr. Protoc. Hum. Genet."},{"key":"2026083118460292500_cit43","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1007\/s10545-012-9510-1","article-title":"Erratum to: Clinical features and\nheteroplasmy in blood, urine and saliva in 34 Dutch families carrying\nthe m.3243A&gt;G mutation","volume":"35","author":"de Laat","year":"2012","journal-title":"J. Inherited Metab.\nDis."},{"key":"2026083118460292500_cit44","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1086\/316930","article-title":"Decrease of\n3243 A &gt; G mtDNA mutation from blood in MELAS syndrome: a longitudinal\nstudy","volume":"68","author":"Rahman","year":"2001","journal-title":"Am. J. Hum. Genet."},{"key":"2026083118460292500_cit45","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1002\/ana.25525","article-title":"Pathogenic variants in MT-ATP6: A United Kingdom-based\nmitochondrial disease cohort study","volume":"86","author":"Ng","year":"2019","journal-title":"Ann. Neurol."},{"key":"2026083118460292500_cit46","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.0000000000213462","article-title":"Natural History of Patients With Mitochondrial\nATPase Deficiency Due to Pathogenic Variants of MT-ATP6 and MT-ATP8","volume":"104","author":"Carli","year":"2025","journal-title":"Neurology"},{"key":"2026083118460292500_cit47","doi-asserted-by":"publisher","first-page":"1392","DOI":"10.1021\/jacsau.5c00011","article-title":"PAM-Independent CRISPR-Cas12a System for Specific\nAssays\nof Single Nucleotide Variants","volume":"5","author":"Ai","year":"2025","journal-title":"JACS Au"},{"key":"2026083118460292500_cit48","doi-asserted-by":"publisher","first-page":"16682","DOI":"10.1021\/acs.analchem.6c02495","article-title":"Detection of Ultralow-Frequency ctDNA Mutations Using\na Dual Hairpin-Competition CRISPR\/Cas14a System","volume":"98","author":"Liu","year":"2026","journal-title":"Anal. Chem."},{"key":"2026083118460292500_cit49","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.70666","article-title":"Nucleic Acid\nNanotechnology-Empowered CRISPR-Cas12a Systems for Biosensing and\nBioimaging Applications","volume":"10","author":"Hou","year":"2026","journal-title":"Small Methods"},{"key":"2026083118460292500_cit50","doi-asserted-by":"publisher","first-page":"118280","DOI":"10.1016\/j.trac.2025.118280","article-title":"Advances in DNA-empowered membrane\nsurface engineering for artificial manipulation and visual analysis\nof cell-cell communication","volume":"190","author":"Zhang","year":"2025","journal-title":"TrAC, Trends Anal.\nChem."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c04072","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/33\/24507\/66543144\/acs.analchem.6c04072.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/33\/24507\/66543144\/acs.analchem.6c04072.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:46:10Z","timestamp":1788216370000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/33\/24507\/5255260\/Visual-Detection-and-Stratification-of-Pathogenic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":50,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,14]]},"published-print":{"date-parts":[[2026,8,25]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c04072","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}