{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:01:23Z","timestamp":1790694083328,"version":"4.1.0"},"reference-count":23,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"The Science and Technology Development Plan Project of Jilin Province","award":["SKL202402017"],"award-info":[{"award-number":["SKL202402017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rapid and accurate genotyping of Bordetella pertussis (BP) is essential for guiding antimicrobial therapy and epidemiological surveillance. However, existing genotyping methods often involve multistep workflows and postamplification handling, increasing the risk of aerosol contamination and limiting their applicability in resource-limited settings. Here, we present a closed-tube, one-pot strategy that integrates loop-mediated isothermal amplification (LAMP) with an enzyme-free catalytic hairpin assembly (CHA) circuit for the simultaneous detection and genotyping of wild-type BP and the antibiotic-resistance A2047G mutant. In this platform, LAMP provides rapid and efficient isothermal amplification, while the CHA circuit directly converts amplification products into fluorescence signals and enables single-nucleotide discrimination through sequence-specific recognition, thereby improving genotyping accuracy without increasing assay complexity. The integrated assay combines rapid detection, high specificity, and a simple closed-tube workflow with minimal instrumentation requirements, making it well suited for point-of-care testing and applications in resource-limited settings.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04126","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:01:25Z","timestamp":1789750885000},"page":"27822-27827","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Identification\nof Antibiotic-Resistance Mutation\nin\n                    <i>Bordetella pertussis<\/i>\n                    Using a One-Pot\nIsothermal System"],"prefix":"10.1021","volume":"98","author":[{"given":"Chunxu","family":"Yu","sequence":"first","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , , ,","place":["Changchun, Jilin, China, 130022"]},{"name":"University of Science and Technology of China , , , ,","place":["Hefei, Anhui, China, 230026"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Zhai","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University , , , ,","place":["Changchun, Jilin, China, 130021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Li","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , , ,","place":["Changchun, Jilin, China, 130022"]},{"name":"University of Science and Technology of China , , , ,","place":["Hefei, Anhui, China, 230026"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidan","family":"Tang","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , , ,","place":["Changchun, Jilin, China, 130022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rujian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , , ,","place":["Changchun, Jilin, China, 130022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University , , , ,","place":["Changchun, Jilin, China, 130021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baiyang","family":"Lu","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , , ,","place":["Changchun, Jilin, China, 130022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7224-0041","authenticated-orcid":true,"given":"Bingling","family":"Li","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , , ,","place":["Changchun, Jilin, China, 130022"]},{"name":"University of Science and Technology of China , , , ,","place":["Hefei, Anhui, China, 230026"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"2026092909111593700_cit1","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1128\/CMR.00083-15","article-title":"Pertussis: Microbiology,\nDisease, Treatment, and Prevention","volume":"29","author":"Kilgore","year":"2016","journal-title":"Clin. Microbiol.\nRev."},{"key":"2026092909111593700_cit2","doi-asserted-by":"publisher","first-page":"S310","DOI":"10.1093\/infdis\/jiaa469","article-title":"Pertussis (Whooping\nCough)","volume":"224","author":"Decker","year":"2021","journal-title":"J.\nInfect. Dis."},{"key":"2026092909111593700_cit3","doi-asserted-by":"publisher","first-page":"1439","DOI":"10.1016\/j.cmi.2024.08.016","article-title":"Pertussis upsurge, age shift and vaccine escape\npost-COVID-19 caused by ptxP3 macrolide-resistant Bordetella pertussis\nMT28 clone in China","volume":"30","author":"Fu","year":"2024","journal-title":"Clin. Microbiol. Infect."},{"key":"2026092909111593700_cit4","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.3390\/antibiotics11111570","article-title":"Macrolide Resistance\nin Bordetella pertussis: Current Situation and Future Challenges","volume":"11","author":"Ivaska","year":"2022","journal-title":"Antibiotics"},{"key":"2026092909111593700_cit5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138941","article-title":"Variation in Bordetella\npertussis Susceptibility to Erythromycin and Virulence-Related Genotype\nChanges in China (1970\u20132014)","volume":"10","author":"Yang","year":"2015","journal-title":"PLoS One"},{"key":"2026092909111593700_cit6","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1101\/SQB.1986.051.01.032","article-title":"Specific enzymatic amplification\nof DNA in vitro: the polymerase chain reaction","volume":"51","author":"Mullis","year":"1986","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"2026092909111593700_cit7","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1080\/07388551.2022.2030295","article-title":"Isothermal nucleic acid\namplification technology for rapid detection of virus","volume":"43","author":"Wei","year":"2023","journal-title":"Crit. Rev. Biotechnol."},{"key":"2026092909111593700_cit8","doi-asserted-by":"publisher","first-page":"12491","DOI":"10.1021\/acs.chemrev.5b00428","article-title":"Isothermal Amplification\nof Nucleic Acids","volume":"115","author":"Zhao","year":"2015","journal-title":"Chem. Rev."},{"key":"2026092909111593700_cit9","doi-asserted-by":"publisher","first-page":"531","DOI":"10.3390\/genes12040531","article-title":"Genotyping of the Major SARS-CoV-2 Clade by Short-Amplicon High-Resolution\nMelting (SA-HRM) Analysis","volume":"12","author":"Diaz-Garcia","year":"2021","journal-title":"Genes"},{"key":"2026092909111593700_cit10","doi-asserted-by":"publisher","first-page":"2102","DOI":"10.1021\/acs.analchem.2c05112","article-title":"Machine Learning-Assisted\nReal-Time Polymerase Chain Reaction and High-Resolution Melt Analysis\nfor SARS-CoV-2 Variant Identification","volume":"95","author":"Promja","year":"2023","journal-title":"Anal.\nChem."},{"key":"2026092909111593700_cit11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal\namplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"2026092909111593700_cit12","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1039\/C9AY02246E","article-title":"Loop-mediated\nisothermal\namplification (LAMP) \u2013 review and classification of methods\nfor sequence-specific detection","volume":"12","author":"Becherer","year":"2020","journal-title":"Anal. Methods"},{"key":"2026092909111593700_cit13","doi-asserted-by":"publisher","first-page":"100440","DOI":"10.1016\/j.snr.2026.100440","article-title":"Loop-mediated Isothermal\nAmplification (LAMP): Current progress and future directions in rapid\nnucleic acid detection","volume":"11","author":"Yang","year":"2026","journal-title":"Sens. Actuators Rep."},{"key":"2026092909111593700_cit14","doi-asserted-by":"publisher","first-page":"8371","DOI":"10.1021\/ac301944v","article-title":"Adapting enzyme-free DNA circuits\nto the detection of loop-mediated isothermal amplification reactions","volume":"84","author":"Li","year":"2012","journal-title":"Anal. Chem."},{"key":"2026092909111593700_cit15","doi-asserted-by":"publisher","first-page":"3314","DOI":"10.1021\/ac504387c","article-title":"Robust Strand Exchange\nReactions for the Sequence-Specific, Real-Time Detection of Nucleic\nAcid Amplicons","volume":"87","author":"Jiang","year":"2015","journal-title":"Anal. Chem."},{"key":"2026092909111593700_cit16","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.aca.2019.06.048","article-title":"A signal-flexible gene diagnostic strategy\ncoupling loop-mediated isothermal amplification with hybridization\nchain reaction","volume":"1079","author":"Dong","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"2026092909111593700_cit17","doi-asserted-by":"publisher","first-page":"139641","DOI":"10.1016\/j.snb.2026.139641","article-title":"A dual-gated detection system for pathogen\ndiagnosis: Integrating nanozyme-based visual screening with duplex-specific\nnuclease-assisted specific verification","volume":"455","author":"Guo","year":"2026","journal-title":"Sens.\nActuators B: Chem."},{"key":"2026092909111593700_cit18","doi-asserted-by":"publisher","first-page":"116582","DOI":"10.1016\/j.trac.2022.116582","article-title":"Catalytic hairpin assembly as cascade nucleic\nacid circuits for fluorescent biosensor: Design, evolution and application","volume":"151","author":"Luo","year":"2022","journal-title":"TrAC, Trends Anal. Chem."},{"key":"2026092909111593700_cit19","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.2.e5","article-title":"A new class of homogeneous\nnucleic acid probes based on specific displacement hybridization","volume":"30","author":"Li","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"2026092909111593700_cit20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr504","article-title":"Rational, modular adaptation of enzyme-free\nDNA circuits to multiple detection methods","volume":"39","author":"Li","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"2026092909111593700_cit21","doi-asserted-by":"publisher","first-page":"36605","DOI":"10.1038\/srep36605","article-title":"Strand-Exchange Nucleic Acid Circuitry\nwith Enhanced Thermo-and Structure- Buffering Abilities Turns Gene\nDiagnostics Ultra-Reliable and Environmental Compatible","volume":"6","author":"Zhu","year":"2016","journal-title":"Sci. Rep."},{"key":"2026092909111593700_cit22","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1039\/C7SC03190D","article-title":"Establishment of a universal and rational gene\ndetection strategy through three-way junction-based remote transduction","volume":"9","author":"Tang","year":"2018","journal-title":"Chem. Sci."},{"key":"2026092909111593700_cit23","doi-asserted-by":"publisher","first-page":"162464","DOI":"10.1016\/j.cej.2025.162464","article-title":"A dual-position nucleic acid coding strategy\nfor one-pot recognition of a pathogen and multiple drug-resistant\nmutations","volume":"512","author":"Yu","year":"2025","journal-title":"Chem. Eng. J."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c04126","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/27822\/68558503\/acs.analchem.6c04126.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/27822\/68558503\/acs.analchem.6c04126.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:39:12Z","timestamp":1790692752000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/38\/27822\/5432939\/Rapid-Identification-of-Antibiotic-Resistance"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":23,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,18]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c04126","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}