{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:42:15Z","timestamp":1792784535637,"version":"4.5.0"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"Interdisciplinary Research Center of Beijing University of Chemical Technology","award":["XK2025-05"],"award-info":[{"award-number":["XK2025-05"]}],"id":[{"id":"https:\/\/ror.org\/00df5yc52","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["22474007"],"award-info":[{"award-number":["22474007"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Accurate discrimination of lung cancer subtypes and evaluation of chemotherapeutic efficacy remain critical challenges in precision oncology owing to the heterogeneity of tumor cellular microenvironments. Herein, we report a mitochondrial microenvironment-responsive fluorescence sensor array, MR-SA, for intelligent analysis of lung cancer cells and chemotherapeutic efficacy. Four mitochondria-targeting fluorescent probes were rationally designed by integrating indolium salts with environment-sensitive fluorophores, enabling selective sensing of mitochondrial hypoxia, polarity, pH, and viscosity in living cells. Benefiting from distinct cross-reactive fluorescence response patterns and broad emission coverage ranging from 573 to 734 nm, MR-SA generated multidimensional fluorescence fingerprints for different lung cancer subtypes while minimizing spectral crosstalk. The chemotherapy-induced mitochondrial alterations were monitored using MR-SA and combined with outlier analysis for evaluation of chemotherapeutic efficacy and drug resistance in nonsmall cell lung cancer cells. Furthermore, MR-SA fluorescence images were integrated with ResNet, a deep-learning framework, enabling rapid and accurate blind classification of lung cancer cells with a recognition accuracy of 99.71%. Thus, MR-SA successfully distinguished lung adenocarcinoma, lung squamous cell carcinoma, large cell carcinoma, and small cell lung cancer cells through differential mitochondrial microenvironment profiling. This work establishes a versatile fluorescence sensing strategy for multidimensional mitochondrial analysis and provides a promising analytical platform for intelligent cancer cell profiling and chemotherapeutic evaluation.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04157","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:02:21Z","timestamp":1789678941000},"page":"28405-28417","source":"Crossref","is-referenced-by-count":0,"title":["A Mitochondrial\nMicroenvironment-Responsive Fluorescent\nSensor Array for Intelligent Analysis of Lung Cancer Cells and Chemotherapeutic\nEfficacy"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0642-9155","authenticated-orcid":true,"given":"Guoyang","family":"Zhang","sequence":"first","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]},{"name":"China Academy of Chinese Medical Sciences , , ,","place":["Beijing, China, 100053"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manman","family":"Xu","sequence":"additional","affiliation":[{"name":"China Academy of Chinese Medical Sciences , , ,","place":["Beijing, China, 100053"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Zhu","sequence":"additional","affiliation":[{"name":"China Academy of Chinese Medical Sciences , , ,","place":["Beijing, China, 100053"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingguang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pulong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingtao","family":"Pu","sequence":"additional","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0952-093X","authenticated-orcid":true,"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"China Academy of Chinese Medical Sciences , , ,","place":["Beijing, China, 100053"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2858-7646","authenticated-orcid":true,"given":"Zhuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing University of Chemical Technology , , ,","place":["Beijing, China, 100029"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"issue":"2","key":"2026092909112748200_cit1","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1053\/j.semnuclmed.2025.02.009","article-title":"PET\/MRI in Non-Small\nCell Lung Cancer (NSCLC)","volume":"55","author":"Alorfi","year":"2025","journal-title":"Semin. 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