{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:44:18Z","timestamp":1792784658271,"version":"4.5.0"},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"National Natural Science Foundation of China (NSFC)","award":["21874022"],"award-info":[{"award-number":["21874022"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["22274022"],"award-info":[{"award-number":["22274022"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["22574023"],"award-info":[{"award-number":["22574023"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Early and accurate determination of human epidermal growth factor receptor 2 (HER2) is of great significance for the diagnosis and prognosis evaluation of breast cancer. Herein, a structurally transformed Fe-doped hexagon-like CuCo Prussian blue analogue (FeCuCoPBAH) with enhanced peroxidase (POD)-like activity was developed as a nanozyme signal amplifier for dual-mode immunosensing of HER2. Structural characterization indicated that the structural transformation was associated with changes in the local chemical environment of Cu sites and redistribution of Cu oxidation states, which correlated with improved apparent POD-like catalytic performance. Benefiting from the enhanced nanozyme activity, FeCuCoPBAH promoted the TMB\/H2O2 chromogenic reaction and showed improved apparent catalytic kinetics compared with the parent materials. On the basis of a glucose oxidase (GOx)-mediated cascade catalytic reaction, a sandwich immunoassay was constructed in which glucose oxidase generated H2O2 in situ, followed by FeCuCoPBAH-catalyzed oxidation of 3,3\u2032,5,5\u2032-tetramethylbenzidine (TMB) to generate two independently optimized colorimetric and electrochemical readouts based on the same TMB\/oxTMB cascade chemistry. Under optimized conditions, the colorimetric mode exhibited a linear response toward HER2 over the range of 0.02\u201320 ng mL\u20131 with a detection limit of 8.8 pg mL\u20131, while the electrochemical mode achieved the linear range of 0.004\u201320 ng mL\u20131 with a lower detection limit of 3.8 pg mL\u20131. In addition, the proposed immunosensor demonstrated excellent selectivity and satisfactory analytical performance. This work provides an effective strategy for enhancing nanozyme activity through solvent regulation and offers a versatile dual-mode sensing platform for sensitive biomarker analysis.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04348","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:02:08Z","timestamp":1788922928000},"page":"27575-27584","source":"Crossref","is-referenced-by-count":0,"title":["Solvent-Engineered Fe-Doped CuCo Prussian Blue Analogue\nNanozyme with Enhanced Catalytic Activity for Dual-Mode Colorimetric\nand Electrochemical Immunoassays"],"prefix":"10.1021","volume":"98","author":[{"given":"Shuqun","family":"Lao","sequence":"first","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhui","family":"Liang","sequence":"additional","affiliation":[{"name":"Mengchao Hepatobiliary Hospital of Fujian Medical University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Entai","family":"Sheng","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Xu","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Kong","sequence":"additional","affiliation":[{"name":"Mengchao Hepatobiliary Hospital of Fujian Medical University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0134-3983","authenticated-orcid":true,"given":"Dianping","family":"Tang","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"2026092208083511000_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2026.103137","article-title":"Treatments\nfor human epidermal growth factor receptor 2 (HER2)\u2013altered\ncancers evaluated using a tumor-agnostic approach: A narrative review","volume":"147","author":"Harada","year":"2026","journal-title":"Cancer Treat. Rev."},{"key":"2026092208083511000_cit2","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1038\/s43018-025-00969-4","article-title":"p95HER2, a truncated\nform of the HER2 oncoprotein,\ndrives an immunosuppressive program in HER2+ breast cancer\nthat limits trastuzumab deruxtecan efficacy","volume":"6","author":"Hu","year":"2025","journal-title":"Nat. Cancer"},{"key":"2026092208083511000_cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117689","article-title":"Dual-metal\nCuFe\/Fe cube nanozyme with Prussian blue analogue for highly efficient\ncolorimetric immunoassay","volume":"287","author":"Lao","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"2026092208083511000_cit4","doi-asserted-by":"publisher","first-page":"13663","DOI":"10.1021\/acs.analchem.4c02606","article-title":"Bimetallic\nsingle-atom nanozyme-based\nelectrochemical-photothermal dual-function portable immunoassay with\nsmartphone imaging","volume":"96","author":"Wang","year":"2024","journal-title":"Anal. Chem."},{"key":"2026092208083511000_cit5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2026.118747","article-title":"Integrated Magnetophoretic\u2013Electrochemical\nplatforms for portable detection of HER2 in breast cancer diagnosis","volume":"308","author":"Anekrattanasap","year":"2026","journal-title":"Biosens. Bioelectron."},{"key":"2026092208083511000_cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114850","article-title":"Photofuel\ncell-based\nself-powered biosensor for HER2 detection by integration of plasmonic-metal\/conjugated\nmolecule hybrids and electrochemical sandwich structure","volume":"220","author":"Bai","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"2026092208083511000_cit7","doi-asserted-by":"publisher","first-page":"1783","DOI":"10.1021\/acs.analchem.5c07715","article-title":"Fe\u2013N3S single-atom nanozyme with asymmetric coordination\nfor ultra-Low-background\ncolorimetric immunoassays","volume":"98","author":"Yu","year":"2026","journal-title":"Anal. 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Chem."},{"key":"2026092208083511000_cit32","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/j.crci.2014.01.017","article-title":"Application of the\ninfrared spectroscopy to the structural study of Prussian blue analogues","volume":"17","author":"Lejeune","year":"2014","journal-title":"C. R. Chim."},{"key":"2026092208083511000_cit33","doi-asserted-by":"publisher","first-page":"1506","DOI":"10.1038\/s41596-018-0001-1","article-title":"Standardized assays for determining the catalytic activity and kinetics\nof peroxidase-like nanozymes","volume":"13","author":"Jiang","year":"2018","journal-title":"Nat. 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