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CN nanosheets work as an excellent ECL luminophore, while AuPt TNPs enhance catalytic activity and electron-transfer capability and boost ECL emission. The AuPt\/CN-modified glassy carbon electrode (AuPt\/CN\/GCE) produces a strong initial ECL signal, i.e., \u201con\u201d status. Subsequent binding of ferrocene (Fc)-labeled hairpin DNA (H-Fc) via Au\u2013S bonds, brings the -Fc group close to AuPt\/CN\/GCE, resulting in the quenching of the ECL signal of H-Fc\/AuPt\/CN\/GCE, i.e., \u201coff\u201d status. When miRNA-499 is present, the Exo III-assisted recycling amplification strategy is activated: miRNA-499 hybridizes with H-Fc to form miRNA-499\/H-Fc\/AuPt\/CN\/GCE, and Exo III selectively cleaves the blunt 3\u2032-terminus of H-Fc. As a result, the Fc group is removed from the AuPt\/CN\/GCE surface, while miRNA-499 is released to trigger the next amplification cycle and restore the ECL signal, i.e., \u201con\u201d status. The as-designed ECL biosensor can ultrasensitively detect miRNA-499 with a detection limit (LOD) of 0.07 fM (S\/N = 3) in the range of 0.1 fM to 10 nM.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04421","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:02:15Z","timestamp":1789462935000},"page":"28511-28518","source":"Crossref","is-referenced-by-count":0,"title":["A Novel \u201cOn\u2013Off\u2013On\u201d\nElectrochemiluminescence\nBiosensor from Multifunctional AuPt Triangular Nanoprisms\/Carbon Nitride\nNanosheets"],"prefix":"10.1021","volume":"98","author":[{"given":"Fang-Fang","family":"Xin","sequence":"first","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxian","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]},{"name":"Hunan Normal University , , , ,","place":["Changsha, Hunan, China, 410013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Futing","family":"Wang","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujin","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changxiao","family":"Song","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Long","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7314-7351","authenticated-orcid":true,"given":"Yifan","family":"Lyu","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Yang","sequence":"additional","affiliation":[{"name":"The University of Western Australia , , , ,","place":["Perth, Western Australia, Australia, 6009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2583-7133","authenticated-orcid":true,"given":"Ren","family":"Cai","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8066-1524","authenticated-orcid":true,"given":"Weihong","family":"Tan","sequence":"additional","affiliation":[{"name":"Hunan University , , , ,","place":["Changsha, Hunan, China, 410082"]},{"name":"Chinese Academy of Sciences , , , ,","place":["Hangzhou, Zhejiang, China, 310022"]},{"name":"Shanghai Jiao Tong University , , ,","place":["Shanghai, China, 200240"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"2026092909111588500_cit1","doi-asserted-by":"publisher","first-page":"117910","DOI":"10.1016\/j.bios.2025.117910","article-title":"Ultra-sensitive\nsolid-state nanochannel electrochemical biosensor based on interfacial\ncharge density modulation and signal synergistic amplification for\nmicroRNA-155 detection","volume":"289","author":"Chen","year":"2025","journal-title":"Biosens. 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