{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:06:16Z","timestamp":1790690776337,"version":"4.1.0"},"reference-count":40,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"publisher","award":["MR\/Y015223\/1"],"award-info":[{"award-number":["MR\/Y015223\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFE0106300"],"award-info":[{"award-number":["2024YFE0106300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009558","name":"University Natural Science Research Project of Anhui Province","doi-asserted-by":"publisher","award":["2024AH050461"],"award-info":[{"award-number":["2024AH050461"]}],"id":[{"id":"10.13039\/501100009558","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["PA2025GDSK0067"],"award-info":[{"award-number":["PA2025GDSK0067"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22406001"],"award-info":[{"award-number":["22406001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["2408085MC067"],"award-info":[{"award-number":["2408085MC067"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Foodborne pathogens represent a persistent threat to global public health, necessitating the development of point-of-care testing (POCT) platforms that integrate rapidity, ultrahigh sensitivity, cost effectiveness, and operational simplicity. Herein, we developed a system for high-specificity identification of foodborne pathogens and associated antibiotic resistance genes, which is a dual-modal diagnostic platform integrating recombinase polymerase amplification (RPA) with an engineered LwaCas13a variant. By leveraging the CRISPR\/Cas13a system, the platform establishes a multiplex fluorescence array that simultaneously detects four major foodborne pathogens (Salmonella enterica serovar typhimurium, Listeria monocytogenes, Escherichia coli, and Staphylococcus aureus) and their corresponding critical antibiotic resistance genes (ARGs), including mecA, blaCTX-M, mcr-1, ermB, and tet(M). Notably, the system retained robust trans-cleavage activity in complex biological matrices, achieving a remarkable limit of detection (LOD) at 100 copies\/mL. Furthermore, the CRISPR\/Cas13a system is coupled to an instrument-free lateral flow assay (LFA) for the detection of L. monocytogenes and tet(M), which effectively circumvents matrix-induced viscosity and ionic inhibition in egg and milk samples. Moreover, our developed platform was validated using samples from 40 household refrigerators, which drastically reduces assay time and thereby circumvents the inhibitory effects that constrain qPCR in food matrices and achieves 100% diagnostic agreement compared with the gold-standard methods. Collectively, this dual-modal strategy provides a promising paradigm with point-of-care capability for food safety surveillance and antimicrobial resistance monitoring.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04448","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:03:06Z","timestamp":1789462986000},"page":"28519-28529","source":"Crossref","is-referenced-by-count":0,"title":["Multiplexed\nDetection of Foodborne Pathogens and the\nAntibiotic Resistance Genes Based on the CRISPR\/Cas System with Engineered\nLwaCas13a"],"prefix":"10.1021","volume":"98","author":[{"given":"Xinlu","family":"Li","sequence":"first","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichun","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Sun","sequence":"additional","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianing","family":"Cao","sequence":"additional","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[{"name":"Queen\u2019s University Belfast , , 19 Chlorine Gardens , ,","place":["Belfast, U.K., BT9 5DL"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongning","family":"Wu","sequence":"additional","affiliation":[{"name":"China National Center for Food Safety Risk Assessment , , ,","place":["Beijing, China, 100017"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0824-0821","authenticated-orcid":true,"given":"Feifei","family":"Sun","sequence":"additional","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"issue":"12","key":"2026092909113779100_cit1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001923","article-title":"World Health Organization global\nestimates and regional comparisons of the burden of foodborne disease\nin 2010","volume":"12","author":"Havelaar","year":"2015","journal-title":"PLoS Med."},{"key":"2026092909113779100_cit2","doi-asserted-by":"publisher","first-page":"111515","DOI":"10.1016\/j.ijfoodmicro.2025.111515","article-title":"Prevalence, antimicrobial\nresistance, and genetic characterization\nof Listeria monocytogenes in the Korean pork production\nchain","volume":"446","author":"Lee","year":"2026","journal-title":"Int. J. Food Microbiol."},{"key":"2026092909113779100_cit3","doi-asserted-by":"publisher","first-page":"111441","DOI":"10.1016\/j.ijfoodmicro.2025.111441","article-title":"Microbiome\nand pathogen identification, and associated\nantimicrobial resistance genes and virulence factors in seafood revealed\nby 16S rRNA amplicon and metagenomic sequencing","volume":"443","author":"Gao","year":"2025","journal-title":"Int. J. Food Microbiol."},{"key":"2026092909113779100_cit4","doi-asserted-by":"publisher","first-page":"109769","DOI":"10.1016\/j.microc.2023.109769","article-title":"Viability dyes quantitative PCR (vPCR)\nassays targeting foodborne pathogens - scientific prospecting (2010\u20132022)","volume":"197","author":"dos Reis Lemos","year":"2024","journal-title":"Microchem. J."},{"key":"2026092909113779100_cit5","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.bios.2018.09.078","article-title":"Rapid detection\nof Salmonella enterica in food samples by a novel\napproach with combination of sample concentration and direct PCR","volume":"129","author":"Vinayaka","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"2026092909113779100_cit6","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/2050-7771-2-13","article-title":"Circumventing qPCR\ninhibition to amplify miRNAs in\nplasma","volume":"2","author":"Plieskatt","year":"2014","journal-title":"Biomark Res."},{"issue":"11","key":"2026092909113779100_cit7","doi-asserted-by":"publisher","first-page":"2839","DOI":"10.3390\/foods10112839","article-title":"Real-time PCR method\nfor the Rapid detection and quantification\nof pathogenic Staphylococcus species based on novel\nmolecular target genes","volume":"10","author":"Kim","year":"2021","journal-title":"Foods"},{"issue":"1","key":"2026092909113779100_cit8","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1038\/s41467-023-36874-6","article-title":"CRISPR-induced DNA\nreorganization for multiplexed nucleic acid detection","volume":"14","author":"Karlikow","year":"2023","journal-title":"Nat. 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Hazard.\nMater."},{"issue":"2","key":"2026092909113779100_cit25","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.cell.2020.12.001","article-title":"Amplification-free detection of SARS-CoV-2\nwith CRISPR-Cas13a and mobile phone microscopy","volume":"184","author":"Fozouni","year":"2021","journal-title":"Cell"},{"key":"2026092909113779100_cit26","doi-asserted-by":"publisher","first-page":"116239","DOI":"10.1016\/j.bios.2024.116239","article-title":"Improving\ntrans-cleavage activity of CRISPR-Cas13a using engineered crRNA with\na uridinylate-rich 5\u2019-overhang","volume":"255","author":"Yang","year":"2024","journal-title":"Biosens.\nBioelectron."},{"key":"2026092909113779100_cit27","doi-asserted-by":"publisher","first-page":"118983","DOI":"10.1016\/j.bios.2026.118983","article-title":"One-Tube RPA-CRISPR-Cas13a assay\nwith rational design for single-molecule detection of waterborne viruses\nin drinking water treatment","volume":"311","author":"Wang","year":"2026","journal-title":"Biosens. 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