{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:03:22Z","timestamp":1790082202354,"version":"4.0.1"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22404020"],"award-info":[{"award-number":["22404020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20232007"],"award-info":[{"award-number":["BK20232007"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20241303"],"award-info":[{"award-number":["BK20241303"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020789","name":"Shuangchuang Program of Jiangsu Province","doi-asserted-by":"publisher","award":["JSSCTD202409"],"award-info":[{"award-number":["JSSCTD202409"]}],"id":[{"id":"10.13039\/501100020789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Full-time Talents Program of Hebei Province","doi-asserted-by":"publisher","award":["2023HBQZYCXY027"],"award-info":[{"award-number":["2023HBQZYCXY027"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The detection of enterokinase (ENTK) activity is essential for the investigation of ENTK-associated diseases, including cancer. However, existing methods are often limited by complex procedures or autofluorescence interference. Here, we report a bioluminescent probe, Ac-Asp-Asp-Asp-Asp-Lys-Luc (DDDDK-Luc), for real-time and sensitive imaging of ENTK activity both in vitro and in vivo. Characterized by a high signal-to-noise ratio and low detection limit, the probe exhibited a 59.7-fold bioluminescence enhancement upon ENTK cleavage in vitro with a detection limit of 5.58 U\/L. Furthermore, DDDDK-Luc enabled real-time monitoring of endogenous ENTK activity in living HeLa cells and in a subcutaneous tumor xenograft mouse model. We anticipate that this probe holds great potential for applications in biopharmaceutical process control and ENTK-related disease diagnosis.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04546","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:01:45Z","timestamp":1789117305000},"page":"27267-27271","source":"Crossref","is-referenced-by-count":0,"title":["An Enterokinase-Activatable\nBioluminescent Probe for\n                    <i>In Vivo<\/i>\n                    Tumor Imaging"],"prefix":"10.1021","volume":"98","author":[{"given":"Xiaotong","family":"Cheng","sequence":"first","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guowei","family":"Liang","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiantian","family":"Xia","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Dong","family":"Xu","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6159-9999","authenticated-orcid":true,"given":"Gaolin","family":"Liang","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]},{"name":"Handan Norman Technology Co., Ltd. , ,","place":["Guantao, China, 057750"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"issue":"5","key":"2026092208072618700_cit1","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1038\/s41570-022-00373-x","article-title":"Designing bioresponsive nanomaterials\nfor intracellular self-assembly","volume":"6","author":"Chagri","year":"2022","journal-title":"Nat. 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