{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:52:04Z","timestamp":1791895924565,"version":"4.3.4"},"reference-count":27,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22134004"],"award-info":[{"award-number":["22134004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374091"],"award-info":[{"award-number":["22374091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["2021ZDSYS09"],"award-info":[{"award-number":["2021ZDSYS09"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019JQ07"],"award-info":[{"award-number":["ZR2019JQ07"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>As the primary risk factor for cardiovascular events, early diagnosis of atherosclerosis (AS) is essential for reducing their incidence. Abnormal accumulation of lipid droplets (LDs) and excessive production of superoxide anion (O2\u2022\u2013) are key synergistic drivers that promote the early progression of AS. This study developed a dual-functional fluorescent nanoprobe, termed LDN-O, for simultaneous imaging and assessment of changes in LDs and O2\u2022\u2013 levels during the onset and progression of AS. LDN-O was fabricated via nanoprecipitation to encapsulate two responsive molecules: LDB-O-ME enables high-fidelity targeted imaging of LDs, while LN-SF-O allows highly selective detection of O2\u2022\u2013. In vitro experiments confirmed that LDN-O exhibits excellent sensitivity and specificity for both targets. Dynamic monitoring with LDN-O revealed that during foam cell formation, the levels of LDs and O2\u2022\u2013 were positively correlated with the duration of oxidized low-density lipoprotein (ox-LDL) stimulation, providing direct experimental evidence for understanding the intrinsic drivers driving early pathological progression in AS. Ex vivo imaging of aortas was further performed in mouse models of early-stage AS and in a combined model of AS with steatohepatitis. The results showed that LDs and O2\u2022\u2013 levels in aortic tissue were significantly higher in the model groups than in the control group, with an even more pronounced increase in the group with concurrent steatohepatitis. Overall, LDN-O enables visualization of lipid deposition and oxidative stress in early AS, offering a new strategy for its early diagnosis.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04547","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:02:17Z","timestamp":1790341337000},"page":"29366-29377","source":"Crossref","is-referenced-by-count":0,"title":["Dual-Functional\nFluorescent Nanoprobe Tracks Lipid\nDroplet Accumulation and Superoxide Anion in Early Atherosclerotic\nLesions"],"prefix":"10.1021","volume":"98","author":[{"given":"Xiaoyu","family":"Gu","sequence":"first","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xia","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2356-0962","authenticated-orcid":true,"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3455-8390","authenticated-orcid":true,"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qilu Hospital of Shandong University , , ,","place":["Jinan, China, 250100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5362-7569","authenticated-orcid":true,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8712-7025","authenticated-orcid":true,"given":"Bo","family":"Tang","sequence":"additional","affiliation":[{"name":"Shandong Normal University , , , ,","place":["Jinan, Shandong, P. R. China, 250014"]},{"name":"Laoshan Laboratory , 168Wenhai Middle Rd, Aoshanwei , Jimo, , ,","place":["Qingdao, Shandong, P. R. China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"2026101308065691300_cit1","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.jconrel.2019.03.025","article-title":"Macrophage-targeted,\nenzyme-triggered fluorescence switch-on system for detection of embolism-vulnerable\natherosclerotic plaques","volume":"302","author":"Narita","year":"2019","journal-title":"J. Controlled Release"},{"issue":"3","key":"2026101308065691300_cit2","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/0735-1097(94)90772-2","article-title":"Triggers, acute\nrisk factors and vulnerable plaques: The lexicon of a new frontier","volume":"23","author":"Muller","year":"1994","journal-title":"J. Am. Coll Cardiol."},{"issue":"10","key":"2026101308065691300_cit3","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1016\/j.jacc.2013.11.034","article-title":"High-Intensity Signals\nin Coronary Plaques on Noncontrast T1-Weighted\nMagnetic Resonance Imaging as a Novel Determinant of Coronary Events","volume":"63","author":"Noguchi","year":"2014","journal-title":"J. Am. Coll Cardiol."},{"issue":"1","key":"2026101308065691300_cit4","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202315861","article-title":"Prediction\nof Early Atherosclerotic Plaques Using a Sequence-Activated Fluorescence\nProbe for the Simultaneous Detection of \u03b3-Glutamyl Transpeptidase\nand Hypobromous Acid","volume":"63","author":"Wang","year":"2024","journal-title":"Angew. Chem., Int. Ed."},{"issue":"3","key":"2026101308065691300_cit5","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1038\/s41556-026-01890-3","article-title":"Mitochondria contact\nlipid droplets through the mitochondrial import complex binding to\nlipid metabolism enzyme Ayr1","volume":"28","author":"Heinen","year":"2026","journal-title":"Nat. Cell Biol."},{"key":"2026101308065691300_cit6","doi-asserted-by":"publisher","first-page":"213577","DOI":"10.1016\/j.ccr.2020.213577","article-title":"Fluorescent\nprobes for the imaging of lipid droplets in live cells","volume":"427","author":"Tian","year":"2021","journal-title":"Coord. Chem. Rev."},{"issue":"12","key":"2026101308065691300_cit7","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1016\/j.tcb.2020.09.006","article-title":"Lipid Metabolism\nin Regulation of Macrophage Functions","volume":"30","author":"Yan","year":"2020","journal-title":"Trends\nCell Biol."},{"issue":"6","key":"2026101308065691300_cit8","doi-asserted-by":"publisher","first-page":"2945","DOI":"10.1002\/ptr.7002","article-title":"Natural products:\nThe role and mechanism in low-density lipoprotein oxidation and atherosclerosis","volume":"35","author":"Zhang","year":"2021","journal-title":"Phytother. Res."},{"key":"2026101308065691300_cit9","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.vph.2018.08.002","article-title":"Foam cell\nformation: A new target\nfor fighting atherosclerosis and cardiovascular disease","volume":"112","author":"Maguire","year":"2019","journal-title":"Vasc. Pharmacol."},{"issue":"2","key":"2026101308065691300_cit10","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1007\/s11883-012-0228-1","article-title":"Current Concepts\nof the Role of Oxidized LDL Receptors in Atherosclerosis","volume":"14","author":"Goyal","year":"2012","journal-title":"Curr. Atheroscler. Rep."},{"issue":"2","key":"2026101308065691300_cit11","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.coph.2008.12.017","article-title":"The monocyte\/macrophage\nas a therapeutic\ntarget in atherosclerosis","volume":"9","author":"Saha","year":"2009","journal-title":"Curr. Opin. 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Med."},{"issue":"29","key":"2026101308065691300_cit22","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202204518","article-title":"In-Sequence High-Specificity Dual-Reporter\nUnlocking of Fluorescent Probe Enables the Precise Identification\nof Atherosclerotic Plaques","volume":"61","author":"Ye","year":"2022","journal-title":"Angew. Chem., Int.\nEd."},{"issue":"10","key":"2026101308065691300_cit23","doi-asserted-by":"publisher","first-page":"4391","DOI":"10.1021\/acs.analchem.0c04019","article-title":"Naphthalimide-4-(4-nitrophenyl)thiosemicarbazide: A\nFluorescent Probe for Simultaneous Monitoring of Viscosity and Nitric\nOxide in Living Cells","volume":"93","author":"Kim","year":"2021","journal-title":"Anal. Chem."},{"key":"2026101308065691300_cit24","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.cca.2013.06.006","article-title":"Foam cells\nin atherosclerosis","volume":"424","author":"Yu","year":"2013","journal-title":"Clin. Chim. 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Med."},{"issue":"5","key":"2026101308065691300_cit27","doi-asserted-by":"publisher","first-page":"166698","DOI":"10.1016\/j.bbadis.2023.166698","article-title":"Myeloid-restricted\nCD68 deficiency attenuates atherosclerosis via inhibition of ROS-MAPK-apoptosis\naxis","volume":"1869","author":"Duan","year":"2023","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c04547","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/40\/29366\/68651254\/acs.analchem.6c04547.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/40\/29366\/68651254\/acs.analchem.6c04547.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:16:57Z","timestamp":1791893817000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/40\/29366\/5438404\/Dual-Functional-Fluorescent-Nanoprobe-Tracks-Lipid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":27,"journal-issue":{"issue":"40","published-online":{"date-parts":[[2026,9,25]]},"published-print":{"date-parts":[[2026,10,13]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c04547","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]}}}