{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:26:36Z","timestamp":1792175196190,"version":"4.4.0"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21874022"],"award-info":[{"award-number":["21874022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274022"],"award-info":[{"award-number":["22274022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22574023"],"award-info":[{"award-number":["22574023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Antioxidants play critical roles in food quality, nutritional value, and health promotion, yet their rapid discrimination in complex food matrices remains challenging because of their diverse structures and redox behaviors. Herein, a defect-rich PtCu (D-PtCu) alloy nanozyme with enhanced peroxidase-like activity was developed to construct a multichannel colorimetric sensor array. By integration of three chromogenic substrates, distinct response fingerprints were generated for six representative food antioxidants. Combined with linear discriminant analysis, the sensor array achieved an accurate classification and identification of different antioxidants. Furthermore, the ascorbic acid contents determined by the proposed method agreed well with high-performance liquid chromatography (HPLC) results, and characteristic antioxidant fingerprints enabled the reliable discrimination of real food samples. This work provides a simple and rapid strategy for antioxidant profiling and food quality evaluation, demonstrating the potential of nanozyme-based sensor arrays for food analysis and nutritional assessments.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04595","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T20:02:39Z","timestamp":1788897759000},"page":"27626-27635","source":"Crossref","is-referenced-by-count":1,"title":["Colorimetric\nFingerprinting of Food Antioxidants and\nQuality Assessment with Defect-Rich PtCu Alloy Nanozyme-Based Sensing\nArray"],"prefix":"10.1021","volume":"98","author":[{"given":"Man","family":"Xu","sequence":"first","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxuan","family":"Cai","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichao","family":"Yu","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqun","family":"Lao","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Entai","family":"Sheng","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4487-1047","authenticated-orcid":true,"given":"Yu","family":"He","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0134-3983","authenticated-orcid":true,"given":"Dianping","family":"Tang","sequence":"additional","affiliation":[{"name":"Fuzhou University , , ,","place":["Fuzhou, People\u2019s Republic of China, 350108"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"2026092208065539600_cit1","doi-asserted-by":"publisher","first-page":"100626","DOI":"10.1016\/j.afres.2024.100626","article-title":"Handmade green\ntea: Antioxidant content and activity","volume":"4","author":"Atak","year":"2024","journal-title":"Appl.\nFood Res."},{"key":"2026092208065539600_cit2","doi-asserted-by":"publisher","first-page":"101698","DOI":"10.1016\/j.fochx.2024.101698","article-title":"Metabolic\nbasis for superior antioxidant capacity of red-fleshed\npeaches","volume":"23","author":"Zhao","year":"2024","journal-title":"Food Chem. X"},{"key":"2026092208065539600_cit3","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1111\/1541-4337.13170","article-title":"Antioxidant\npotential and factors influencing the content of antioxidant compounds\nof pepper: A review with current knowledge","volume":"22","author":"Sanatombi","year":"2023","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"2026092208065539600_cit4","doi-asserted-by":"publisher","first-page":"20470","DOI":"10.1021\/acs.jafc.4c04961","article-title":"New utilization\nof fraxinus mandshurica leaves: As\na safe and promising natural antioxidant","volume":"72","author":"Guo","year":"2024","journal-title":"J.\nAgric. Food Chem."},{"key":"2026092208065539600_cit5","doi-asserted-by":"publisher","first-page":"879","DOI":"10.3390\/antiox13070879","article-title":"Recent advances in\nastaxanthin as an antioxidant in\nfood applications","volume":"13","author":"Dang","year":"2024","journal-title":"Antioxidant"},{"key":"2026092208065539600_cit6","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.molcel.2023.11.001","article-title":"Regulation of antioxidants\nin cancer","volume":"84","author":"Hecht","year":"2024","journal-title":"Mol. Cell"},{"key":"2026092208065539600_cit7","doi-asserted-by":"publisher","first-page":"907","DOI":"10.3390\/antiox12040907","article-title":"The importance of natural antioxidants\nin female reproduction","volume":"12","author":"Vas\u030ckova\u0301","year":"2023","journal-title":"Antioxidants"},{"key":"2026092208065539600_cit8","doi-asserted-by":"publisher","first-page":"117522","DOI":"10.1016\/j.trac.2024.117522","article-title":"Advancements\nin metal organic framework-based\nmaterials for the detection of antioxidants in food and biological\nfluids","volume":"171","author":"Kumar","year":"2024","journal-title":"TrAC, Trends Anal. Chem."},{"key":"2026092208065539600_cit9","doi-asserted-by":"publisher","first-page":"143876","DOI":"10.1016\/j.foodchem.2025.143876","article-title":"Metal-organic framework-based\nnanozymes for water-soluble antioxidants\nand Total antioxidant capacity detection: Principles and applications","volume":"479","author":"Ameen","year":"2025","journal-title":"Food Chem."},{"key":"2026092208065539600_cit10","doi-asserted-by":"publisher","first-page":"141348","DOI":"10.1016\/j.foodchem.2024.141348","article-title":"Recent advances in\nthe analytical methods for quantitative determination of antioxidants\nin food matrices","volume":"463","author":"Patil","year":"2025","journal-title":"Food Chem."},{"key":"2026092208065539600_cit11","doi-asserted-by":"publisher","first-page":"215427","DOI":"10.1016\/j.ccr.2023.215427","article-title":"Metal\/covalent-organic\nframeworks-based electrochemical sensors for the detection of ascorbic\nacid, dopamine and uric acid","volume":"497","author":"Liang","year":"2023","journal-title":"Coord. Chem. Rev."},{"key":"2026092208065539600_cit12","doi-asserted-by":"publisher","first-page":"341968","DOI":"10.1016\/j.aca.2023.341968","article-title":"Pyrylium-based\nderivatization for rapid labeling and enhanced detection of thiol\nin mass spectrometry imaging","volume":"1284","author":"Huang","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"2026092208065539600_cit13","doi-asserted-by":"publisher","first-page":"343389","DOI":"10.1016\/j.aca.2024.343389","article-title":"Pioneering\nfood analysis: Transformative antioxidant testing with colorimetric\nassays using Co\u2013Mn bimetallic oxide nanozyme","volume":"1333","author":"Chen","year":"2025","journal-title":"Anal. Chim. Acta"},{"key":"2026092208065539600_cit14","doi-asserted-by":"publisher","first-page":"118619","DOI":"10.1016\/j.microc.2026.118619","article-title":"A molecularly imprinted cerium-engineered metal\u2013organic\nframework platform for colorimetric determination of trichlorfon","volume":"227","author":"Alaseem","year":"2026","journal-title":"Microchem. J."},{"issue":"21","key":"2026092208065539600_cit15","doi-asserted-by":"publisher","first-page":"4387","DOI":"10.1039\/D6AY00462H","article-title":"A novel peroxidase-mimicking Cu,S co-doped carbon dot\nnanozyme for dual-mode lactate biosensing in serum and sweat","volume":"18","author":"Alaseem","year":"2026","journal-title":"Anal. Methods"},{"key":"2026092208065539600_cit16","doi-asserted-by":"publisher","first-page":"105004","DOI":"10.1016\/j.tifs.2025.105004","article-title":"Enzyme-inspired\nnanosensing of food antioxidants: From targeted quantification to\nsystem-level analysis","volume":"160","author":"Huang","year":"2025","journal-title":"Trends In Food Sci. Technol."},{"key":"2026092208065539600_cit17","doi-asserted-by":"publisher","first-page":"19957","DOI":"10.1021\/acs.analchem.5c04478","article-title":"Kinetic and mechanistic\ndiscrepancies of single\/dual-atom nanozymes drive a triple-channel\nsensing array for machine learning-assisted antioxidant discrimination","volume":"97","author":"Shen","year":"2025","journal-title":"Anal. Chem."},{"key":"2026092208065539600_cit18","doi-asserted-by":"publisher","first-page":"148058","DOI":"10.1016\/j.foodchem.2026.148058","article-title":"Vitamin C in fruits and vegetables:\nstability, processing effects, analytical detection, nutrient interactions,\nbioavailability, and health implications","volume":"505","author":"Verma","year":"2026","journal-title":"Food\nChem."},{"key":"2026092208065539600_cit19","doi-asserted-by":"publisher","first-page":"128100","DOI":"10.1016\/j.saa.2026.128100","article-title":"A bioinspired\nnanozyme cascade sensor for fluorometric\nand smartphone-assisted colorimetric cholesterol analysis in serum\nand food samples","volume":"361","author":"Ali","year":"2026","journal-title":"Spectrochim. Acta, Part A"},{"key":"2026092208065539600_cit20","doi-asserted-by":"publisher","first-page":"113635","DOI":"10.1016\/j.diamond.2026.113635","article-title":"Electrospun FeN@CNT single-atom nanozyme\nfor dual-mode\nROS-mediated colorimetric and fluorometric detection of vitamin C","volume":"165","author":"Alasiri","year":"2026","journal-title":"Diam. Related Mater."},{"key":"2026092208065539600_cit21","doi-asserted-by":"publisher","first-page":"6555","DOI":"10.1021\/acs.analchem.4c06063","article-title":"Single-site\niridium catalyst on metal\u2013organic framework as light-responsive\noxidase-like nanozyme with high stability for colorimetric detection\nof antioxidant capacity","volume":"97","author":"Zhou","year":"2025","journal-title":"Anal. Chem."},{"key":"2026092208065539600_cit22","doi-asserted-by":"publisher","first-page":"138648","DOI":"10.1016\/j.snb.2025.138648","article-title":"MnO2 nanosheets\nas Mn2+ donors for driving the DNAzyme and oxidase mimics\nratiometric fluorescent biosensor: Ultrasensitive total antioxidant\ncapacity assay","volume":"446","author":"Liu","year":"2026","journal-title":"Sens. Actuators, B"},{"key":"2026092208065539600_cit23","doi-asserted-by":"publisher","first-page":"24013","DOI":"10.1021\/acsnano.5c06349","article-title":"Modulating p-d orbital\nhybridization in mesoporous medium-entropy alloy nanozymes with enhanced\nperoxidase-like activity","volume":"19","author":"Zhang","year":"2025","journal-title":"ACS Nano"},{"key":"2026092208065539600_cit24","doi-asserted-by":"publisher","first-page":"11463","DOI":"10.1021\/acs.analchem.4c01912","article-title":"Multi-enzyme\ncascade\nnanoreactors for high-throughput immunoassay: transitioning concept\nin lab to application in community","volume":"96","author":"Yu","year":"2024","journal-title":"Anal. Chem."},{"issue":"7","key":"2026092208065539600_cit25","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s00604-026-08179-0","article-title":"Superior oxidase-mimetic\ngadolinium vanadate nanoparticles for sensitive enzyme-free colorimetric\ndetection of uric acid","volume":"193","author":"Asran","year":"2026","journal-title":"Microchim. Acta"},{"key":"2026092208065539600_cit26","doi-asserted-by":"publisher","first-page":"17865","DOI":"10.1021\/acs.analchem.5c03805","article-title":"Bifunctional block\npolymer-engineered platinum nanozymes for ultrasensitive immunoassay","volume":"97","author":"Xu","year":"2025","journal-title":"Anal. Chem."},{"issue":"2","key":"2026092208065539600_cit27","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202414625","article-title":"D-band-center-engineered\nplatinum-based\nnanozyme for personalized pharmacovigilance","volume":"64","author":"Yu","year":"2025","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026092208065539600_cit28","doi-asserted-by":"publisher","first-page":"17700","DOI":"10.1021\/acsami.5c21428","article-title":"Construction of defect-rich ordered\nPtCu nanooctahedrons to enhance oxygen reduction electrocatalysis","volume":"18","author":"Zhang","year":"2026","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026092208065539600_cit29","doi-asserted-by":"publisher","first-page":"172469","DOI":"10.1016\/j.cej.2025.172469","article-title":"Support and\nalloy engineering enabling highly efficient peroxidase mimicking for\nthe ultrasensitive detection of Escherichia coli O157: H7","volume":"529","author":"Xu","year":"2026","journal-title":"Chem. Eng.\nJ."},{"key":"2026092208065539600_cit30","doi-asserted-by":"publisher","first-page":"217967","DOI":"10.1016\/j.ccr.2026.217967","article-title":"Defect-engineered nanozymes:\nUnlocking biocatalytic strategies for\nnext-generation anti-infection therapy","volume":"562","author":"Li","year":"2026","journal-title":"Coord.\nChem. Rev."},{"key":"2026092208065539600_cit31","doi-asserted-by":"publisher","first-page":"118678","DOI":"10.1016\/j.microc.2026.118678","article-title":"Oxygen-vacancy-rich\nEu-MOF@CoNiFe3O4 magnetic nanozyme for ultrasensitive\ndual-mode detection\nof uric acid","volume":"227","author":"Ali","year":"2026","journal-title":"Microchem. J."},{"key":"2026092208065539600_cit32","doi-asserted-by":"publisher","first-page":"217067","DOI":"10.1016\/j.ccr.2025.217067","article-title":"Recent advancements for defect engineering\nin nanozymes","volume":"546","author":"Han","year":"2026","journal-title":"Coord. Chem. Rev."},{"key":"2026092208065539600_cit33","doi-asserted-by":"publisher","first-page":"142837","DOI":"10.1016\/j.foodchem.2025.142837","article-title":"A novel fluorescent\nsensor for highly sensitive detection\nof ascorbic acid in food based on inhibiting phosphatase-like activity\nof Zr-based MOF","volume":"471","author":"Wu","year":"2025","journal-title":"Food Chem."},{"key":"2026092208065539600_cit34","doi-asserted-by":"publisher","first-page":"144349","DOI":"10.1016\/j.foodchem.2025.144349","article-title":"Manganese-doped\ncarbon nanospheres with robust peroxidase-like activity for the colorimetric\ndetection of total antioxidant capacity","volume":"484","author":"Wang","year":"2025","journal-title":"Food\nChem."},{"key":"2026092208065539600_cit35","doi-asserted-by":"publisher","first-page":"15586","DOI":"10.1021\/acs.analchem.6c00690","article-title":"Active ruthenium-based\nhigh-entropy nanozyme through a \u201cchemical tongue\u201d for\nrecognizing bioactive components in honeysuckle","volume":"98","author":"Nie","year":"2026","journal-title":"Anal. Chem."},{"key":"2026092208065539600_cit36","doi-asserted-by":"publisher","first-page":"137385","DOI":"10.1016\/j.snb.2025.137385","article-title":"Machine learning-assisted three-dimensional\nfluorescence for heavy metal multi-sensing","volume":"431","author":"Chen","year":"2025","journal-title":"Sens. Actuators, B"},{"key":"2026092208065539600_cit37","doi-asserted-by":"publisher","first-page":"137158","DOI":"10.1016\/j.snb.2024.137158","article-title":"Dual-site\nperoxidase-mimic graphdiyne-based colorimetric sensor arrays with\nmachine learning for screening of multiple antibiotics","volume":"427","author":"Qiu","year":"2025","journal-title":"Sens. Actuators, B"},{"key":"2026092208065539600_cit38","doi-asserted-by":"publisher","first-page":"43438","DOI":"10.1021\/jacs.5c11454","article-title":"Conductive covalent organic frameworks\nas chemiresistive sensor arrays for the detection and differentiation\nof gasotransmitters","volume":"147","author":"Benedetto","year":"2025","journal-title":"J. Am. Chem. Soc."},{"key":"2026092208065539600_cit39","doi-asserted-by":"publisher","first-page":"24115","DOI":"10.1021\/acs.analchem.5c04619","article-title":"Engineering a metal\u2013organic\nframework-dominated bioinspired multienzymatic sensor array for portable\ndetection of perfluoroalkyl substances","volume":"97","author":"Wang","year":"2025","journal-title":"Anal.\nChem."},{"key":"2026092208065539600_cit40","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1021\/acs.analchem.5c05867","article-title":"Ce-CDs-loaded MIL-53(Fe)\nnanozymes multisubstrate sensor array integrated with multiple machine\nlearning complementary strategy for highly reliable antioxidant discrimination\nand determination","volume":"98","author":"Wei","year":"2026","journal-title":"Anal. Chem."},{"key":"2026092208065539600_cit41","doi-asserted-by":"publisher","first-page":"10023","DOI":"10.1021\/jacs.4c00791","article-title":"Precise tuning of the d-band center\nof dual-atomic enzymes for catalytic therapy","volume":"146","author":"Zeng","year":"2024","journal-title":"J. Am. Chem. Soc."},{"issue":"9","key":"2026092208065539600_cit42","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13394","article-title":"Concept, mechanism,\nand applications of phenolic antioxidants in foods","volume":"44","author":"Zeb","year":"2020","journal-title":"J. Food Biochem."},{"key":"2026092208065539600_cit43","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.3390\/ijms21031131","article-title":"ABTS\/PP decolorization\nassay of antioxidant capacity reaction pathways","volume":"21","author":"Ilyasov","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"2026092208065539600_cit44","doi-asserted-by":"publisher","first-page":"8505","DOI":"10.1007\/s00216-016-9976-z","article-title":"Pitfalls and\ncapabilities of various hydrogen donors in evaluation of peroxidase-like\nactivity of gold nanoparticles","volume":"408","author":"Drozd","year":"2016","journal-title":"Anal. Bioanal.\nChem."},{"issue":"1","key":"2026092208065539600_cit45","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1038\/s43586-024-00346-y","article-title":"Linear discriminant\nanalysis","volume":"4","author":"Zhao","year":"2024","journal-title":"Nat. Rev. Methods Primers"}],"container-title":["Analytical\nChemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c04595","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/37\/27626\/68453249\/acs.analchem.6c04595.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/37\/27626\/68453249\/acs.analchem.6c04595.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:30:32Z","timestamp":1790080232000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/37\/27626\/5426645\/Colorimetric-Fingerprinting-of-Food-Antioxidants"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":45,"journal-issue":{"issue":"37","published-online":{"date-parts":[[2026,9,8]]},"published-print":{"date-parts":[[2026,9,22]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c04595","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,8]]}}}