{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:54:45Z","timestamp":1791294885292,"version":"4.1.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20232007"],"award-info":[{"award-number":["BK20232007"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22404020"],"award-info":[{"award-number":["22404020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["225B2402"],"award-info":[{"award-number":["225B2402"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Full-time Talents Program of Hebei Province","award":["2023HBQZYCXY027"],"award-info":[{"award-number":["2023HBQZYCXY027"]}]},{"name":"Jiangsu Shuang Chuang Team","award":["JSSCTD202409"],"award-info":[{"award-number":["JSSCTD202409"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fluorescence probes that enable sensitive and real-time in vivo imaging of matrix metalloproteinase-9 (MMP-9) hold high promise for early clinical diagnosis of intractable rheumatoid arthritis (RA), but they remain scarcely developed. Herein, we developed the dually quenched fluorescence nanoprobe P-IR780-NP for sensitive in vivo imaging of RA. To prepare P-IR780-NP, a molecular precursor Cys(StBu)-Pro-Leu-Gly-Leu-Lys(IR780)-CBT (P-IR780) was synthesized. Under reductive conditions, P-IR780 undergoes a 2-cyanobenzothiazole-cysteine (CBT-Cys) click reaction to form a cyclic dimer (P-IR780-Dimer), which subsequently assembles into P-IR780-NP nanoparticles. The sequential cyclization and assembly processes cause intramolecular and intermolecular quenching of the conjugated IR780 fluorophore, respectively, thereby collectively minimizing background fluorescence. In vitro studies confirmed that MMP-9 specifically cleaved P-IR780-Dimer, triggering nanoparticle disassembly and consequent 8.6-fold fluorescence enhancement. In MMP-9-overexpressing macrophages, P-IR780-NP produced 1.8-fold stronger fluorescence signals compared to the MMP-9-inhibited control group. Notably, in vivo imaging of RA mice revealed a 3.7-fold higher fluorescence intensity in affected joints relative to healthy controls, demonstrating the enhanced sensitivity of P-IR780-NP for in vivo RA imaging. We anticipate that P-IR780-NP could be applied for early clinical diagnosis of RA in the near future.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04651","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:01:22Z","timestamp":1789768882000},"page":"28623-28628","source":"Crossref","is-referenced-by-count":0,"title":["Matrix Metalloproteinase-9-Activated\nDually-Quenched\nFluorescence Nanoprobe for Sensitive Imaging of Rheumatoid Arthritis"],"prefix":"10.1021","volume":"98","author":[{"given":"Hai-Dong","family":"Xu","sequence":"first","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Cheng","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaochu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runqun","family":"Tang","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8649-7809","authenticated-orcid":true,"given":"Xiaoyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiancheng","family":"Fu","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1120-3623","authenticated-orcid":true,"given":"Xianbao","family":"Sun","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6159-9999","authenticated-orcid":true,"given":"Gaolin","family":"Liang","sequence":"additional","affiliation":[{"name":"Southeast University , , ,","place":["Nanjing, China, 211189"]},{"name":"Handan Norman Technology Co., Ltd , ,","place":["Guantao, China, 057750"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"issue":"2","key":"2026100608083439600_cit1","doi-asserted-by":"publisher","first-page":"102735","DOI":"10.1016\/j.autrev.2020.102735","article-title":"Beyond the joints,\nthe extra-articular manifestations in rheumatoid arthritis","volume":"20","author":"Conforti","year":"2021","journal-title":"Autoimmun. Rev."},{"key":"2026100608083439600_cit2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2022-070856","article-title":"Therapeutic advances\nin rheumatoid\narthritis","volume":"384","author":"Brown","year":"2024","journal-title":"Br. Med. J."},{"issue":"7987","key":"2026100608083439600_cit3","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1038\/s41586-023-06708-y","article-title":"Deconstruction of rheumatoid\narthritis synovium defines inflammatory subtypes","volume":"623","author":"Zhang","year":"2023","journal-title":"Nature"},{"issue":"13","key":"2026100608083439600_cit4","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.1001\/jama.2018.13103","article-title":"Diagnosis and Management of Rheumatoid\nArthritis: A\nReview","volume":"320","author":"Aletaha","year":"2018","journal-title":"JAMA"},{"key":"2026100608083439600_cit5","doi-asserted-by":"publisher","first-page":"1275","DOI":"10.1038\/s41467-018-03691-1","article-title":"Towards\nan arthritis flare-responsive drug delivery\nsystem","volume":"9","author":"Joshi","year":"2018","journal-title":"Nat. Commun."},{"issue":"6","key":"2026100608083439600_cit6","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1038\/s41584-023-00966-w","article-title":"Matrix metalloproteinases\nin arthritis:\ntowards precision medicine","volume":"19","author":"Grillet","year":"2023","journal-title":"Nat. Rev. Rheumatol."},{"issue":"1","key":"2026100608083439600_cit7","doi-asserted-by":"publisher","first-page":"2174","DOI":"10.1038\/s41467-021-22454-z","article-title":"Targeted apoptosis\nof macrophages and osteoclasts in arthritic joints is effective against\nadvanced inflammatory arthritis","volume":"12","author":"Deng","year":"2021","journal-title":"Nat. Commun."},{"issue":"1","key":"2026100608083439600_cit8","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1038\/s41392-022-01050-7","article-title":"STAT3-NAV2 axis\nas a new therapeutic target for rheumatoid arthritis via activating\nSSH1L\/Cofilin-1 signaling pathway","volume":"7","author":"Wang","year":"2022","journal-title":"Signal Transduction\nTargeted Ther."},{"key":"2026100608083439600_cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2022.101524","article-title":"A novel MMP-responsive\nnanoplatform with transformable magnetic resonance\nproperty for quantitative tumor bioimaging and synergetic chemo-photothermal\ntherapy","volume":"45","author":"Chen","year":"2022","journal-title":"Nano Today"},{"issue":"9","key":"2026100608083439600_cit10","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1038\/s41569-024-01009-x","article-title":"Uncovering\natherosclerotic cardiovascular disease by\nPET imaging","volume":"21","author":"Maier","year":"2024","journal-title":"Nat. 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Lett."},{"issue":"43","key":"2026100608083439600_cit13","doi-asserted-by":"publisher","first-page":"18294","DOI":"10.1021\/jacs.1c08898","article-title":"An Activatable Near-Infrared\nFluorescence Probe for in Vivo Imaging of Acute Kidney Injury by Targeting\nPhosphatidylserine and Caspase-3","volume":"143","author":"Weng","year":"2021","journal-title":"J. Am. Chem.\nSoc."},{"issue":"37","key":"2026100608083439600_cit14","doi-asserted-by":"publisher","first-page":"25462","DOI":"10.1021\/jacs.4c10606","article-title":"Tandem Targeting and Dual Aggregation of an AIEgen for Enhanced Near-Infrared\nFluorescence Imaging of Tumors","volume":"146","author":"Deng","year":"2024","journal-title":"J. Am. Chem.\nSoc."},{"issue":"50","key":"2026100608083439600_cit15","doi-asserted-by":"publisher","first-page":"27748","DOI":"10.1021\/jacs.3c10255","article-title":"Dual Aggregations of a Near-Infrared\nAggregation-Induced Emission Luminogen for Enhanced Imaging of Alzheimer\u2019s\nDisease","volume":"145","author":"Xu","year":"2023","journal-title":"J. Am. Chem. Soc."},{"issue":"4","key":"2026100608083439600_cit16","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202416877","article-title":"Rational Design of an\nActivatable Near-Infrared Fluorogenic Platform\nfor In Vivo Orthotopic Tumor Imaging and Resection","volume":"64","author":"Wu","year":"2025","journal-title":"Angew. Chem., Int. 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Ed."},{"issue":"11","key":"2026100608083439600_cit28","doi-asserted-by":"publisher","first-page":"19328","DOI":"10.1021\/acsnano.2c08896","article-title":"Granzyme B Turns Nanoparticle\nFluorescence \u201cOn\u201d for Imaging Cytotoxic T Lymphocyte\nActivity in Vivo","volume":"16","author":"Xu","year":"2022","journal-title":"ACS Nano"},{"issue":"24","key":"2026100608083439600_cit29","doi-asserted-by":"publisher","first-page":"7359","DOI":"10.7150\/thno.37625","article-title":"Carboxylesterase-Cleavable Biotinylated\nNanoparticle for Tumor-Dual Targeted Imaging","volume":"9","author":"Chen","year":"2019","journal-title":"Theranostics"},{"key":"2026100608083439600_cit30","doi-asserted-by":"publisher","first-page":"118149","DOI":"10.1016\/j.bios.2025.118149","article-title":"Dually quenched and targeting nanoprobe for sensitive and specific\nfluorescence imaging of tumor","volume":"293","author":"Liang","year":"2026","journal-title":"Biosens. Bioelectron."},{"issue":"30","key":"2026100608083439600_cit31","doi-asserted-by":"publisher","DOI":"10.1002\/anie.1325073","article-title":"A 19F MRI\/NIR-FL Nanoprobe\nfor \u2033Turn-On\u2033\nImaging of \u03b2-Secretase (BACE1) Activity in Alzheimer\u2019s\nDisease","volume":"65","author":"Xu","year":"2026","journal-title":"Angew. Chem., Int. Ed."},{"issue":"1","key":"2026100608083439600_cit32","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s40164-024-00514-6","article-title":"Single-cell\nanalysis defines highly specific leukemia-induced\nneutrophils and links MMP8 expression to recruitment of tumor associated\nneutrophils during FGFR1 driven leukemogenesis","volume":"13","author":"Hu","year":"2024","journal-title":"Exp. Hematol. Oncol."},{"issue":"1","key":"2026100608083439600_cit33","doi-asserted-by":"publisher","first-page":"12786","DOI":"10.1038\/s41598-024-61769-x","article-title":"Sinomenine\ntreats rheumatoid arthritis by inhibiting MMP9 and inflammatory cytokines\nexpression: bioinformatics analysis and experimental validation","volume":"14","author":"Luo","year":"2024","journal-title":"Sci. Rep."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c04651","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/39\/28623\/68562642\/acs.analchem.6c04651.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/39\/28623\/68562642\/acs.analchem.6c04651.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:09:12Z","timestamp":1791292152000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/39\/28623\/5433191\/Matrix-Metalloproteinase-9-Activated-Dually"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":33,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2026,9,18]]},"published-print":{"date-parts":[[2026,10,6]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c04651","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}