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However, current virus detection often faces two challenges: first, due to the complex matrix of physiological samples, detection methods often fail to provide accurate quantitative results; second, conventional PCR-related signal amplification procedures are often challenged by issues such as primer interference and non-specific amplification. Herein, we developed an absolute and amplified virus detection strategy by coupling isotope dilution and catalytic hairpin assembly (CHA). The high-risk cervical cancer-related biomarkers were detected with a wide linear detection range (5-30,000 pM) and detection limits in the pM level. To our best knowledge, this is the first amplified metal isotope dilution quantification strategy for biomolecules.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c04868","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:02:18Z","timestamp":1788537738000},"page":"29099-29107","source":"Crossref","is-referenced-by-count":0,"title":["Absolute and\nAmplified Virus Detection by Isotope\nDilution after Catalytic Hairpin Assembly"],"prefix":"10.1021","volume":"98","author":[{"given":"Jianna","family":"Peng","sequence":"first","affiliation":[{"name":"Sichuan University , , 24 South Section 1, 1st Ring Road , ,","place":["Chengdu, China, 610064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Sichuan University , , 24 South Section 1, 1st Ring Road , ,","place":["Chengdu, China, 610064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinshun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sichuan University , , 24 South Section 1, 1st Ring Road , ,","place":["Chengdu, China, 610064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Hu","sequence":"additional","affiliation":[{"name":"University of Alberta , , 116 St. and 85 Ave. , , ,","place":["Edmonton, Alberta, Canada, T6G 2R3"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9928-5373","authenticated-orcid":true,"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"Sichuan University , , 24 South Section 1, 1st Ring Road , ,","place":["Chengdu, China, 610064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7104-2414","authenticated-orcid":true,"given":"Yi","family":"Lv","sequence":"additional","affiliation":[{"name":"Sichuan University , , 24 South Section 1, 1st Ring Road , ,","place":["Chengdu, China, 610064"]},{"name":"Sichuan University , , 24 South Section 1, 1st Ring Road , ,","place":["Chengdu, China, 610064"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"issue":"4","key":"2026100608082940700_cit1","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/S1470-2045(09)70096-8","article-title":"A review of human carcinogens\u2500Part\nB: biological agents","volume":"10","author":"Bouvard","year":"2009","journal-title":"Lancet Oncol."},{"issue":"8","key":"2026100608082940700_cit2","doi-asserted-by":"publisher","first-page":"5188","DOI":"10.1002\/jmv.27015","article-title":"Prevalence of high-risk\nHPV and its\ngenotypes\u2500Implications in the choice of prophylactic HPV vaccine","volume":"93","author":"Suresh","year":"2021","journal-title":"J. Med. Virol."},{"issue":"2","key":"2026100608082940700_cit3","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.clon.2018.10.004","article-title":"The human papillomavirus\nas a common pathogen in oropharyngeal, anal and cervical cancers","volume":"31","author":"Otter","year":"2019","journal-title":"Clin. Oncol."},{"issue":"6","key":"2026100608082940700_cit4","doi-asserted-by":"publisher","first-page":"3476","DOI":"10.1021\/acs.analchem.2c05320","article-title":"Integrating\nCRISPR-Cas12a into a microfluidic dual-droplet device enables simultaneous\ndetection of HPV16 and HPV18","volume":"95","author":"Zhao","year":"2023","journal-title":"Anal. Chem."},{"key":"2026100608082940700_cit5","doi-asserted-by":"publisher","first-page":"S11","DOI":"10.1016\/j.vaccine.2006.05.111","article-title":"The burden of HPV-related cancers","volume":"24","author":"Parkin","year":"2006","journal-title":"Vaccine"},{"issue":"1","key":"2026100608082940700_cit6","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/s41664-024-00328-9","article-title":"CRISPR-Cas12a\ncoupled with universal\ndual-mode fluorescent nanoparticles platform for HPV nucleic acid\ndetection","volume":"9","author":"Xu","year":"2025","journal-title":"J. Anal. Test."},{"issue":"4","key":"2026100608082940700_cit7","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1002\/ijc.30716","article-title":"Worldwide\nburden of cancer attributable to HPV by site, country and HPV type","volume":"141","author":"De Martel","year":"2017","journal-title":"Int. J. 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Test."},{"key":"2026100608082940700_cit17","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s41664-025-00407-5","article-title":"Application\nof Biosensors Based on\nCovalent Organic Frameworks, Metal-Organic Frameworks, and their Composites\nin Biomedical Detection","volume":"10","author":"Chen","year":"2025","journal-title":"J. Anal. Test."},{"key":"2026100608082940700_cit18","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/s41664-025-00398-3","article-title":"Application of CRISPR-Based\nBiosensors in Analytical Chemistry","volume":"10","author":"Zhou","year":"2026","journal-title":"J. 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Chem."},{"issue":"6","key":"2026100608082940700_cit28","doi-asserted-by":"publisher","first-page":"2769","DOI":"10.1021\/ac203141d","article-title":"Protein quantitation\nusing Ru-NHS ester tagging and isotope dilution high-pressure liquid\nchromatography\u2013inductively coupled plasma mass spectrometry\ndetermination","volume":"84","author":"Liu","year":"2012","journal-title":"Anal. Chem."},{"issue":"12","key":"2026100608082940700_cit29","doi-asserted-by":"publisher","first-page":"6670","DOI":"10.1021\/acs.analchem.4c06637","article-title":"Isotope dilution\nDNA logic circuits for multiple output and absolute quantification","volume":"97","author":"Zhu","year":"2025","journal-title":"Anal. 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Lett."},{"key":"2026100608082940700_cit33","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114490","article-title":"Enzyme-free catalytic hairpin assembly\nreaction-mediated micro-orifice resistance assay for the ultrasensitive\nand low-cost detection of Listeria monocytogenes","volume":"214","author":"Ren","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"28","key":"2026100608082940700_cit34","doi-asserted-by":"publisher","first-page":"8739","DOI":"10.1021\/jacs.8b03956","article-title":"Genetically encoded\ncatalytic hairpin assembly for sensitive RNA\nimaging in live cells","volume":"140","author":"Mudiyanselage","year":"2018","journal-title":"J. Am. Chem. Soc."},{"issue":"10","key":"2026100608082940700_cit35","doi-asserted-by":"publisher","first-page":"12059","DOI":"10.1021\/acsami.1c23227","article-title":"Simultaneous\nimaging of dual microRNAs in cancer cells through catalytic hairpin\nassembly on a DNA tetrahedron","volume":"14","author":"Li","year":"2022","journal-title":"ACS Appl. Mater.\nInterfaces"},{"issue":"51","key":"2026100608082940700_cit36","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202217291","article-title":"Ru\u0308cktitelbild: An Enzymatically Gated Catalytic Hairpin Assembly\nDelivered by Lipid Nanoparticles for the Tumor-Specific Activation\nof Signal Amplification in miRNA Imaging (Angew. Chem. 51\/2022)","volume":"134","author":"Liu","year":"2022","journal-title":"Angew. 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Gynaecol."},{"issue":"3","key":"2026100608082940700_cit45","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1097\/GRF.0000000000000793","article-title":"HPV testing\nand its role in cervical cancer screening","volume":"66","author":"Clarke","year":"2023","journal-title":"Clin.\nObstet. Gynecol."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c04868","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/39\/29099\/68379888\/acs.analchem.6c04868.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/39\/29099\/68379888\/acs.analchem.6c04868.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:24:18Z","timestamp":1791289458000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/39\/29099\/5420938\/Absolute-and-Amplified-Virus-Detection-by-Isotope"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":45,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2026,9,4]]},"published-print":{"date-parts":[[2026,10,6]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c04868","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}