{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T08:22:52Z","timestamp":1793694172478,"version":"4.5.1"},"reference-count":46,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2017,7,16]],"date-time":"2017-07-16T00:00:00Z","timestamp":1500163200000},"content-version":"vor","delay-in-days":47,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccby_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["FC001030"],"award-info":[{"award-number":["FC001030"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["FC001030"],"award-info":[{"award-number":["FC001030"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L018853\/1"],"award-info":[{"award-number":["BB\/L018853\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["FC001030"],"award-info":[{"award-number":["FC001030"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Different influenza virus strains have caused a number of recent outbreaks killing scores of people and causing significant losses in animal farming. Simple, rapid, sensitive, and specific detection of particular strains, such as a pandemic strain versus a previous seasonal influenza, plays a crucial role in the monitoring, controlling, and management of outbreaks. In this paper we describe a dual recognition element lateral flow assay (DRELFA) which pairs a nucleic acid aptamer with an antibody for use as a point-of-care platform which can detect particular strains of interest. The combination is used to overcome the individual limitations of antibodies\u2019 cross-reactivity and aptamers\u2019 slow binding kinetics. In the detection of influenza viruses, we show that DRELFA can discriminate a particular virus strain against others of the same subtype or common respiratory diseases while still exhibiting fast binding kinetic of the antibody-based lateral flow assay (LFA). The improvement in specificity that DRELFA exhibits is an advantage over the currently available antibody-based LFA systems for influenza viruses, which offer discrimination between influenza virus types and subtypes. Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is very effective in localizing the analyte to the test line (consistently over 90%) and this is crucial for the sensitivity of the device. In addition, color intensities of the test lines showed a good correlation between the DRELFA and the qRT-PCR over a 50-fold concentration range. Finally, lateral flow strips with a streptavidin capture test line and an anti-antibody control line are universally applicable to specific detection of a wide range of different analytes.<\/jats:p>","DOI":"10.1021\/acs.analchem.7b01149","type":"journal-article","created":{"date-parts":[[2017,5,30]],"date-time":"2017-05-30T20:13:02Z","timestamp":1496175182000},"page":"6781-6786","source":"Crossref","is-referenced-by-count":120,"title":["Dual Recognition Element Lateral Flow Assay Toward\nMultiplex Strain Specific\nInfluenza Virus Detection"],"prefix":"10.1021","volume":"89","author":[{"given":"Thao T.","family":"Le","sequence":"first","affiliation":[{"name":"Imperial College London , , ,","place":["London, U.K., SW7 2AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengxiang","family":"Chang","sequence":"additional","affiliation":[{"name":"Pirbright Institute , , ,","place":["Woking, U.K., GU24 0NF"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald J.","family":"Benton","sequence":"additional","affiliation":[{"name":"Francis Crick Institute , , ,","place":["London, U.K., NW1 1AT"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"McCauley","sequence":"additional","affiliation":[{"name":"Francis Crick Institute , , ,","place":["London, U.K., NW1 1AT"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munir","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Pirbright Institute , , ,","place":["Woking, U.K., GU24 0NF"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8881-4786","authenticated-orcid":true,"given":"Anthony E. 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