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Because low stability G-quadruplexes are hardly detectable by mass spectrometry, we optimized solvent conditions: isopropanol in a triethylamine\/hexafluoroisopropanol mixture highly increased G-quadruplex sensitivity with no modification of the physiological G-quadruplex conformation. G-quadruplexes\/G-quadruplex-ligand complexes were also correctly detected at concentration as low as 40 nM. Detection of the physiological conformation of G4s and their complexes opens up the possibility to perform high-throughput screening of G-quadruplex ligands for the development of drug molecules effective against critical human diseases.<\/jats:p>","DOI":"10.1021\/acs.analchem.7b01282","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T16:24:13Z","timestamp":1502209453000},"page":"8632-8637","source":"Crossref","is-referenced-by-count":29,"title":["Highly Improved Electrospray Ionization-Mass Spectrometry\nDetection of G-Quadruplex-Folded Oligonucleotides and Their\nComplexes with Small Molecules"],"prefix":"10.1021","volume":"89","author":[{"given":"Matteo","family":"Scalabrin","sequence":"first","affiliation":[{"name":"University of Padua , , via Gabelli 63 , ,","place":["Padua, Italy, 35121"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manlio","family":"Palumbo","sequence":"additional","affiliation":[{"name":"University of Padua , , via Marzolo 5 , ,","place":["Padua, Italy, 35131"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5446-9029","authenticated-orcid":true,"given":"Sara N.","family":"Richter","sequence":"additional","affiliation":[{"name":"University of Padua , , via Gabelli 63 , ,","place":["Padua, Italy, 35121"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2017,8,8]]},"reference":[{"key":"2026090211153155800_cit1","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1016\/0092-8674(89)90610-7","volume":"59","author":"Williamson","year":"1989","journal-title":"Cell"},{"key":"2026090211153155800_cit2","doi-asserted-by":"publisher","first-page":"8112","DOI":"10.1021\/bi00150a002","volume":"31","author":"Wang","year":"1992","journal-title":"Biochemistry"},{"key":"2026090211153155800_cit3","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.sbi.2006.05.011","volume":"16","author":"Phan","year":"2006","journal-title":"Curr. 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