{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T08:20:01Z","timestamp":1793780401168,"version":"4.6.1"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:00:00Z","timestamp":1498089600000},"content-version":"vor","delay-in-days":28,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003151","name":"Fonds de Recherche du Qu\u00e9bec - Nature et Technologies","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100003151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Urinary tract infections (UTI) are one of the most common bacterial infections and would greatly benefit from a rapid point-of-care diagnostic test. Although significant progress has been made in developing microfluidic systems for nucleic acid and whole bacteria immunoassay tests, their practical application is limited by complex protocols, bulky peripherals, and slow operation. Here we present a microfluidic capillaric circuit (CC) optimized for rapid and automated detection of bacteria in urine. Molds for CCs were constructed using previously established design rules, then 3D-printed and replicated into poly(dimethylsiloxane). CCs autonomously and sequentially performed all liquid delivery steps required for the assay. For efficient bacteria capture, on-the-spot packing of antibody-functionalized microbeads was completed in &amp;lt;20 s followed by autonomous sequential delivery of 100 \u03bcL of bacteria sample, biotinylated detection antibodies, fluorescent streptavidin conjugate, and wash buffer for a total volume \u2248115 \u03bcL. The assay was completed in &amp;lt;7 min. Fluorescence images of the microbead column revealed captured bacteria as bright spots that were easily counted manually or using an automated script for user-independent assay readout. The limit of detection of E. coli in synthetic urine was 1.2 \u00d7 102 colony-forming-units per mL (CFU\/mL), which is well below the clinical diagnostic criterion (&amp;gt;105 CFU\/mL) for UTI. The self-powered, peripheral-free CC presented here has potential for use in rapid point-of-care UTI screening.<\/jats:p>","DOI":"10.1021\/acs.analchem.7b01315","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T10:01:49Z","timestamp":1495706509000},"page":"6846-6853","source":"Crossref","is-referenced-by-count":65,"title":["Microfluidic Capillaric Circuit for Rapid and Facile\nBacteria Detection"],"prefix":"10.1021","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4776-1774","authenticated-orcid":true,"given":"Ayokunle Oluwafemi","family":"Olanrewaju","sequence":"first","affiliation":[{"name":"McGill University , , 3775 rue University , , , ,","place":["Montreal, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 740 Dr Penfield Avenue , , , ,","place":["Montreal, QC, Canada, H3A 0G1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Ng","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 rue University , , , ,","place":["Montreal, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 740 Dr Penfield Avenue , , , ,","place":["Montreal, QC, Canada, H3A 0G1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"DeCorwin-Martin","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 rue University , , , ,","place":["Montreal, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 740 Dr Penfield Avenue , , , ,","place":["Montreal, QC, Canada, H3A 0G1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Robillard","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 rue University , , , ,","place":["Montreal, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 740 Dr Penfield Avenue , , , ,","place":["Montreal, QC, Canada, H3A 0G1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7313-1162","authenticated-orcid":true,"given":"David","family":"Juncker","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 rue University , , , ,","place":["Montreal, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 740 Dr Penfield Avenue , , , ,","place":["Montreal, QC, Canada, H3A 0G1"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2017,5,25]]},"reference":[{"key":"2026090116414865800_cit1","doi-asserted-by":"publisher","first-page":"1028","DOI":"10.1056\/NEJMcp1104429","volume":"366","author":"Hooton","year":"2012","journal-title":"N. 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Proteome Res."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.7b01315","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/89\/12\/6846\/67522415\/acs.analchem.7b01315.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/89\/12\/6846\/67522415\/acs.analchem.7b01315.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:41:55Z","timestamp":1788295315000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/89\/12\/6846\/816941\/Microfluidic-Capillaric-Circuit-for-Rapid-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,25]]},"references-count":38,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2017,5,25]]},"published-print":{"date-parts":[[2017,6,20]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.7b01315","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,25]]}}}