{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:15:27Z","timestamp":1789193727373,"version":"build-2803163510"},"reference-count":48,"publisher":"American Chemical Society (ACS)","issue":"15","license":[{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2011-0030043"],"award-info":[{"award-number":["2011-0030043"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1E1A1A02920873"],"award-info":[{"award-number":["2016R1E1A1A02920873"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human carboxylesterase-2 (CE2) is a carboxylesterase that catalyzes the hydrolysis of endogenous and exogenous substrates. Abnormal CE2 levels are associated with various cancers, and CE2 is a key mediator of anticancer prodrugs, including irinotecan. Here, we developed a two-photon ratiometric probe for detecting CE2 activity using succinate ester as a recognition site for CE2. The probe showed high selectivity to CE2, a clear emission color change, high photostability, and bright two-photon microscopy (TPM) imaging capability, allowing the quantitative detection of CE2 activity in live cells. Using TPM ratio analysis, we show for the first time that CE2 activity was much lower in breast cancer cells than in normal cells. In CE2 overexpression studies, cancer cells had a markedly enhanced sensitivity to the cytotoxic effect of irinotecan, corresponding well with the TPM ratio of the probe. These results may provide useful information for quantitatively measuring CE2 activity in situ and predicting the responsiveness to anticancer drugs.<\/jats:p>","DOI":"10.1021\/acs.analchem.8b02101","type":"journal-article","created":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T21:25:05Z","timestamp":1531949105000},"page":"9465-9471","source":"Crossref","is-referenced-by-count":65,"title":["Carboxylesterase-2-Selective Two-Photon Ratiometric\nProbe Reveals Decreased Carboxylesterase\u20112 Activity in Breast\nCancer Cells"],"prefix":"10.1021","volume":"90","author":[{"given":"Sang Jun","family":"Park","sequence":"first","affiliation":[{"name":"Ajou University , , ,","place":["Suwon, Korea, 443-749"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Ji","family":"Kim","sequence":"additional","affiliation":[{"name":"Ajou University , , ,","place":["Suwon, Korea, 443-749"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Su","family":"Kang","sequence":"additional","affiliation":[{"name":"Ajou University , , ,","place":["Suwon, Korea, 443-749"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Ajou University School of Medicine , , ,","place":["Suwon, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong Sook","family":"Choi","sequence":"additional","affiliation":[{"name":"Ajou University School of Medicine , , ,","place":["Suwon, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4112-9009","authenticated-orcid":true,"given":"Hwan Myung","family":"Kim","sequence":"additional","affiliation":[{"name":"Ajou University , , ,","place":["Suwon, Korea, 443-749"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,7,18]]},"reference":[{"key":"2026081803273994800_cit1","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1146\/annurev.pharmtox.38.1.257","volume":"38","author":"Satoh","year":"1998","journal-title":"Annu. 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Dispos."},{"key":"2026081803273994800_cit4","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1080\/07357900701560786","volume":"26","author":"Tang","year":"2008","journal-title":"Cancer Invest."},{"key":"2026081803273994800_cit5","doi-asserted-by":"publisher","first-page":"75279","DOI":"10.18632\/oncotarget.12143","volume":"7","author":"Yu","year":"2016","journal-title":"Oncotarget"},{"key":"2026081803273994800_cit6","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1177\/030089160909500411","volume":"95","author":"Cai","year":"2009","journal-title":"Tumori"},{"key":"2026081803273994800_cit7","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1093\/jnci\/djv132","volume":"107","author":"Capello","year":"2015","journal-title":"J. Natl. Cancer.\nInst."},{"key":"2026081803273994800_cit8","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.fitote.2017.01.010","volume":"117","author":"Wang","year":"2017","journal-title":"Fitoterapia"},{"key":"2026081803273994800_cit9","first-page":"2605","volume":"8","author":"Xu","year":"2002","journal-title":"Clin. Cancer Res."},{"key":"2026081803273994800_cit10","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1038\/sj.bjc.6600519","volume":"87","author":"Oosterhoff","year":"2002","journal-title":"Br. J. 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Sci."},{"key":"2026081803273994800_cit20","doi-asserted-by":"publisher","first-page":"14519","DOI":"10.1039\/C4CC06642A","volume":"50","author":"Feng","year":"2014","journal-title":"Chem.\nCommun."},{"key":"2026081803273994800_cit21","doi-asserted-by":"publisher","first-page":"28474","DOI":"10.1021\/acsami.5b09573","volume":"7","author":"Jin","year":"2015","journal-title":"ACS\nAppl. Mater. Interfaces"},{"key":"2026081803273994800_cit22","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.bios.2016.04.075","volume":"83","author":"Jin","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"2026081803273994800_cit23","doi-asserted-by":"publisher","first-page":"3703","DOI":"10.1039\/C5SC05001D","volume":"7","author":"Park","year":"2016","journal-title":"Chem. 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Postgrad.\nMed."},{"key":"2026081803273994800_cit44","doi-asserted-by":"publisher","first-page":"9779","DOI":"10.1039\/c3cc45250f","volume":"49","author":"Ge","year":"2013","journal-title":"Chem. Commun."},{"key":"2026081803273994800_cit45","doi-asserted-by":"publisher","first-page":"9884","DOI":"10.1021\/acs.analchem.7b01975","volume":"89","author":"Jin","year":"2017","journal-title":"Anal.\nChem."},{"key":"2026081803273994800_cit46","doi-asserted-by":"publisher","first-page":"2795","DOI":"10.1039\/C6SC03970G","volume":"8","author":"Dai","year":"2017","journal-title":"Chem.\nSci."},{"key":"2026081803273994800_cit47","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0006-8993(01)02887-6","volume":"916","author":"Siebert","year":"2001","journal-title":"Brain Res."},{"key":"2026081803273994800_cit48","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1124\/jpet.104.081265","volume":"313","author":"Quinney","year":"2005","journal-title":"J. Pharmacol.\nExp. Ther."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.8b02101","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/90\/15\/9465\/8485982\/ac8b02101.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/90\/15\/9465\/8485982\/ac8b02101.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:57:25Z","timestamp":1787043445000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/90\/15\/9465\/819580\/Carboxylesterase-2-Selective-Two-Photon"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,18]]},"references-count":48,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2018,7,18]]},"published-print":{"date-parts":[[2018,8,7]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.8b02101","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,18]]}}}