{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T14:26:44Z","timestamp":1786804004509,"version":"build-2736575974"},"reference-count":26,"publisher":"American Chemical Society (ACS)","issue":"22","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG020866"],"award-info":[{"award-number":["AG020866"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,11,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Macroautophagy is a complex degradative intracellular process by which long-lived proteins and damaged organelles are cleared. Common methods for the analysis of autophagy are bulk measurements which mask organelle heterogeneity and complicate the analysis of interorganelle association and trafficking. Thus, methods for individual organelle quantification are needed to address these deficiencies. Current techniques for quantifying individual autophagy organelles are either low through-put or are dimensionally limited. We make use of the multiparametric capability of mass cytometry to investigate phenotypic heterogeneity in autophagy-related organelle types that have been isolated from murine brain, liver, and skeletal muscle. Detection and phenotypic classification of individual organelles were accomplished through the use of a lanthanide-chelating membrane stain and organelle-specific antibodies. Posthoc sample matrix background correction and nonspecific antibody binding corrections provide measures of interorganelle associations and heterogeneity. This is the first demonstration of multiparametric individual organelle analysis via mass cytometry. The method described here illustrates the potential for further investigation of the inherently complex interorganelle associations, trafficking, and heterogeneity present in most eukaryotic biological systems.<\/jats:p>","DOI":"10.1021\/acs.analchem.8b02790","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T12:44:44Z","timestamp":1539780284000},"page":"13315-13321","source":"Crossref","is-referenced-by-count":14,"title":["Quantifying Heterogeneity of Individual Organelles\nin Mixed Populations via Mass Cytometry"],"prefix":"10.1021","volume":"90","author":[{"given":"Heather\nM. G.","family":"Brown","sequence":"first","affiliation":[{"name":"University of Minnesota , , , ,","place":["Minneapolis, Minnesota, United States, 55455"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8863-6174","authenticated-orcid":true,"given":"Edgar A.","family":"Arriaga","sequence":"additional","affiliation":[{"name":"University of Minnesota , , , ,","place":["Minneapolis, Minnesota, United States, 55455"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,10,17]]},"reference":[{"issue":"7656","key":"2026081502110161400_cit1","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1038\/nature22369","article-title":"Applying systems-level\nspectral imaging and analysis\nto reveal the organelle interactome","volume":"546","author":"Valm","year":"2017","journal-title":"Nature"},{"issue":"5","key":"2026081502110161400_cit2","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1016\/j.cell.2011.07.030","article-title":"Autophagy and aging","volume":"146","author":"Rubinsztein","year":"2011","journal-title":"Cell"},{"issue":"3","key":"2026081502110161400_cit3","doi-asserted-by":"publisher","first-page":"131","DOI":"10.4161\/auto.1.3.2017","article-title":"Autophagy and Aging: The Importance\nof Maintaining \u2033Clean\u2033 Cells","volume":"1","author":"Cuervo","year":"2005","journal-title":"Autophagy"},{"issue":"9","key":"2026081502110161400_cit4","doi-asserted-by":"publisher","first-page":"935","DOI":"10.4161\/auto.7.9.15760","article-title":"Seeing is believing: The impact of electron microscopy on autophagy\nresearch","volume":"7","author":"Seglen","year":"2011","journal-title":"Autophagy"},{"issue":"23","key":"2026081502110161400_cit5","doi-asserted-by":"publisher","first-page":"11391","DOI":"10.1021\/ac4023549","article-title":"Describing autophagy\nvia analysis of individual organelles\nby capillary electrophoresis with laser induced fluorescence detection","volume":"85","author":"Satori","year":"2013","journal-title":"Anal. 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