{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T19:59:54Z","timestamp":1793908794291,"version":"4.8.0"},"reference-count":53,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr?d","doi-asserted-by":"publisher","award":["245828"],"award-info":[{"award-number":["245828"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr?d","doi-asserted-by":"publisher","award":["272201"],"award-info":[{"award-number":["272201"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005366","name":"Universitetet i Oslo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI137056"],"award-info":[{"award-number":["R01AI137056"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2024,3,5]]},"DOI":"10.1021\/acs.biochem.3c00612","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T15:22:15Z","timestamp":1708010535000},"page":"587-598","source":"Crossref","is-referenced-by-count":19,"title":["Using\n                    <i>Vibrio natriegens<\/i>\n                    for High-Yield Production of Challenging Expression Targets and for Protein Perdeuteration"],"prefix":"10.1021","volume":"63","author":[{"given":"Natalia","family":"Mojica","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Oslo, NO-0315 Blindern, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flore","family":"Kersten","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oslo, NO-0315 Blindern, Oslo, Norway"},{"name":"Centre for Molecular Medicine Norway, University of Oslo, NO-0318 Blindern, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateu","family":"Montserrat-Canals","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oslo, NO-0315 Blindern, Oslo, Norway"},{"name":"Centre for Molecular Medicine Norway, University of Oslo, NO-0318 Blindern, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Robb","family":"Huhn III","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida 32816, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abelone M.","family":"Tislevoll","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oslo, NO-0315 Blindern, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8029-8043","authenticated-orcid":true,"given":"Gabriele","family":"Cordara","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oslo, NO-0315 Blindern, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Teter","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida 32816, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6688-8151","authenticated-orcid":true,"given":"Ute","family":"Krengel","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oslo, NO-0315 Blindern, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2013.10.016"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3668"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00172"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2015.11.001"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-809-9:035"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3970"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1128\/jb.83.4.736-737.1962"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.5.1349-1358.2002"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15128"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02537"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.627181"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejbt.2022.01.003"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.9b00403"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90520-D"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.6994-7003.1997"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1910"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.023069"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00127-R"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1997.1309"},{"key":"ref20\/cit20","volume-title":"Experiments in molecular genetics","author":"Miller J. H.","year":"1972"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48579-2"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c02168"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-016-0052-y"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-03939-9"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/11.7.577"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911007773"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913000061"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444994003112"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910045749"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2015.07.003"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/bi0360152"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911001314"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-483-4_18"},{"key":"ref38\/cit38","unstructured":"SGI-DNA. Vmax\n                      TM\n                      X2 chemically competent\ncells-Instructions. 2020. https:\/\/files.sgidna.com\/docs\/40029-Vmax-Instructions.pdf (accessed Apr 4, 2023)."},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/121.Supplement.S63"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.24.7752"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7109"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.24.12140"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1084\/jem.130.1.185"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1986.tb03037.x"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(88)65027-0"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.2.481"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1293-1574"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2004.06.022"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800188-2.00007-0"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203740200"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308633200"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1038\/nature04249"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02780"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.3c00612","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.3c00612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T04:30:04Z","timestamp":1709613004000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.biochem.3c00612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,15]]},"references-count":53,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,3,5]]}},"alternative-id":["10.1021\/acs.biochem.3c00612"],"URL":"https:\/\/doi.org\/10.1021\/acs.biochem.3c00612","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.11.03.565449","asserted-by":"object"}]},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,15]]}}}