{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:08:22Z","timestamp":1790759302348,"version":"4.1.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"21","license":[{"start":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T00:00:00Z","timestamp":1463011200000},"content-version":"vor","delay-in-days":366,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM069857"],"award-info":[{"award-number":["GM069857"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The newly discovered light-dependent transcription factor CarH uses adenosylcobalamin as a light sensor to regulate expression of protective genes in bacteria upon exposure to sunlight. This use of adenosylcobalamin is a clever adaptation of a classic enzyme cofactor, taking advantage of its photolabile Co\u2013C bond. However, it is also puzzling in that photolysis of adenosylcobalamin generates the 5\u2032-deoxyadenosyl radical that could damage DNA. Here, using liquid chromatography and spectroscopic techniques, we demonstrate that CarH suppresses release of the 5\u2032-deoxyadenosyl radical and instead effects conversion to a nonreactive 4\u2032,5\u2032-anhydroadenosine. In this manner, CarH safeguards use of adenosylcobalamin in light-dependent gene regulation.<\/jats:p>","DOI":"10.1021\/acs.biochem.5b00416","type":"journal-article","created":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T11:52:58Z","timestamp":1431431578000},"page":"3231-3234","source":"Crossref","is-referenced-by-count":83,"title":["The Transcription\nFactor CarH Safeguards Use of Adenosylcobalamin\nas a Light Sensor by Altering the Photolysis Products"],"prefix":"10.1021","volume":"54","author":[{"given":"Marco","family":"Jost","sequence":"first","affiliation":[{"name":"Massachusetts Institute of Technology , , 77 Massachusetts Avenue , , ,","place":["Cambridge, Massachusetts, United States, 02139"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey H.","family":"Simpson","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology , , 77 Massachusetts Avenue , , ,","place":["Cambridge, Massachusetts, United States, 02139"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine L.","family":"Drennan","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology , , 77 Massachusetts Avenue , , ,","place":["Cambridge, Massachusetts, United States, 02139"]},{"name":"Massachusetts Institute of Technology , , 77 Massachusetts Avenue , , ,","place":["Cambridge, Massachusetts, United States, 02139"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2015,5,12]]},"reference":[{"key":"2026090401111886000_cit1","first-page":"501","volume-title":"Comprehensive Natural Products II. Chemistry and Biology","author":"Frey","year":"2010"},{"key":"2026090401111886000_cit2","doi-asserted-by":"crossref","first-page":"7565","DOI":"10.1073\/pnas.1018972108","volume":"108","author":"Ortiz-Guerrero","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026090401111886000_cit3","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/j.1365-2958.2007.06086.x","volume":"67","author":"Perez-Marin","year":"2008","journal-title":"Mol. Microbiol."},{"key":"2026090401111886000_cit4","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.mib.2010.12.009","volume":"14","author":"Elias-Arnanz","year":"2011","journal-title":"Curr. Opin. Microbiol."},{"key":"2026090401111886000_cit5","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1128\/JB.01125-10","volume":"193","author":"Takano","year":"2011","journal-title":"J. Bacteriol."},{"key":"2026090401111886000_cit6","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1021\/ja00508a066","volume":"101","author":"Endicott","year":"1979","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit7","doi-asserted-by":"crossref","first-page":"12987","DOI":"10.1016\/S0021-9258(19)38257-2","volume":"265","author":"Chen","year":"1990","journal-title":"J. Biol. Chem."},{"key":"2026090401111886000_cit8","doi-asserted-by":"crossref","first-page":"7286","DOI":"10.1021\/ja981029u","volume":"120","author":"Walker","year":"1998","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit9","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1016\/S0021-9258(19)73965-9","volume":"237","author":"Hogenkamp","year":"1962","journal-title":"J. Biol. Chem."},{"key":"2026090401111886000_cit10","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0021-9258(19)84023-1","volume":"238","author":"Hogenkamp","year":"1963","journal-title":"J. Biol. Chem."},{"key":"2026090401111886000_cit11","doi-asserted-by":"crossref","first-page":"7284","DOI":"10.1021\/bi700077v","volume":"46","author":"Schwartz","year":"2007","journal-title":"Biochemistry"},{"key":"2026090401111886000_cit12","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0005-2787(73)90032-4","volume":"331","author":"Law","year":"1973","journal-title":"Biochim. Biophys. Acta"},{"key":"2026090401111886000_cit13","doi-asserted-by":"crossref","first-page":"9735","DOI":"10.1021\/ja0481631","volume":"126","author":"Stich","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit14","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1042\/bj3550131","volume":"355","author":"Huhta","year":"2001","journal-title":"Biochem. J."},{"key":"2026090401111886000_cit15","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1021\/ja00707a047","volume":"92","author":"Schrauzer","year":"1970","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit16","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0006-3061(00)80102-7","volume":"4","author":"Schrauzer","year":"1975","journal-title":"Bioinorg. Chem."},{"key":"2026090401111886000_cit17","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1002\/anie.197605421","volume":"15","author":"Katz","year":"1976","journal-title":"Angew. Chem.,\nInt. Ed."},{"key":"2026090401111886000_cit18","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/S0021-9258(17)38108-5","volume":"253","author":"Krouwer","year":"1978","journal-title":"J. Biol. Chem."},{"key":"2026090401111886000_cit19","doi-asserted-by":"crossref","first-page":"8856","DOI":"10.1021\/ja051527k","volume":"127","author":"Sandala","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit20","doi-asserted-by":"crossref","first-page":"4414","DOI":"10.1021\/ic00072a042","volume":"32","author":"Garr","year":"1993","journal-title":"Inorg. Chem."},{"key":"2026090401111886000_cit21","doi-asserted-by":"crossref","first-page":"10440","DOI":"10.1021\/ja00052a045","volume":"114","author":"Garr","year":"1992","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit23","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1021\/ja00293a037","volume":"107","author":"Jacobsen","year":"1985","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit24","doi-asserted-by":"crossref","first-page":"8335","DOI":"10.1021\/ja00467a045","volume":"99","author":"Sweany","year":"1977","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit25","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1039\/DT9850001613","author":"Baldwin","year":"1985","journal-title":"J. Chem. Soc., Dalton Trans."},{"key":"2026090401111886000_cit26","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.1021\/ja00218a021","volume":"110","author":"Kim","year":"1988","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit27","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1021\/om00160a025","volume":"9","author":"Ng","year":"1990","journal-title":"Organometallics"},{"key":"2026090401111886000_cit29","doi-asserted-by":"crossref","first-page":"4060","DOI":"10.1021\/ja00745a048","volume":"93","author":"Schrauzer","year":"1971","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit30","doi-asserted-by":"crossref","first-page":"4601","DOI":"10.1021\/ja00510a027","volume":"101","author":"Grate","year":"1979","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit31","doi-asserted-by":"crossref","first-page":"4860","DOI":"10.1021\/jacs.5b01838","volume":"137","author":"Lacy","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"2026090401111886000_cit32","doi-asserted-by":"crossref","first-page":"15127","DOI":"10.1073\/pnas.1213442109","volume":"109","author":"Marinescu","year":"2012","journal-title":"Proc. Natl. Acad.\nSci. U.S.A."},{"key":"2026090401111886000_cit33","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/0968-0896(96)00119-8","volume":"4","author":"Jarrett","year":"1996","journal-title":"Bioorg. Med. Chem."},{"key":"2026090401111886000_cit34","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1021\/ja0396910","volume":"126","author":"Sension","year":"2004","journal-title":"J. Am. Chem.\nSoc."},{"key":"2026090401111886000_cit35","doi-asserted-by":"crossref","first-page":"18146","DOI":"10.1021\/jp052492d","volume":"109","author":"Sension","year":"2005","journal-title":"J. Phys. Chem.\nB"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.5b00416","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/bichaw\/article-pdf\/54\/21\/3231\/67277704\/acs.biochem.5b00416.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/bichaw\/article-pdf\/54\/21\/3231\/67277704\/acs.biochem.5b00416.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T14:29:11Z","timestamp":1788791351000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/bichaw\/article\/54\/21\/3231\/1477209\/The-Transcription-Factor-CarH-Safeguards-Use-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,12]]},"references-count":33,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2015,5,12]]},"published-print":{"date-parts":[[2015,6,2]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.biochem.5b00416","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,12]]}}}