{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:47:03Z","timestamp":1786999623747,"version":"build-2736575974"},"reference-count":87,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["2021-15271"],"award-info":[{"award-number":["2021-15271"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["02\/0419\/21\/EMR-II"],"award-info":[{"award-number":["02\/0419\/21\/EMR-II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["CRG-2022-004059"],"award-info":[{"award-number":["CRG-2022-004059"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["IITDH\/R&D\/BD\/202526\/269"],"award-info":[{"award-number":["IITDH\/R&D\/BD\/202526\/269"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004541","name":"Ministry of Education, India","doi-asserted-by":"publisher","award":["STARS-2\/2023-0657"],"award-info":[{"award-number":["STARS-2\/2023-0657"]}],"id":[{"id":"10.13039\/501100004541","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Indian Institute of Technology Dharwad","award":["IITDH\/R&D\/6.81\/SGNF\/2023-24\/169"],"award-info":[{"award-number":["IITDH\/R&D\/6.81\/SGNF\/2023-24\/169"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2026,6,2]]},"DOI":"10.1021\/acs.biochem.6c00271","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:01:48Z","timestamp":1778832108000},"page":"1811-1833","source":"Crossref","is-referenced-by-count":1,"title":["Dimerization of MilM Is Essential for Catalyzing the Pyridoxal-5\u2032-phosphate (PLP)-Dependent C\u03b3-Hydroxylation of\n                    <scp>l<\/scp>\n                    -Arginine during Mildiomycin Biosynthesis"],"prefix":"10.1021","volume":"65","author":[{"given":"Simita","family":"Das","sequence":"first","affiliation":[{"name":"Department of Chemistry","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yashwanth","family":"Naik","sequence":"additional","affiliation":[{"name":"Department of Chemistry","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udita","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahima","family":"Ganguly","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badri Nath","family":"Dubey","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suvamay","family":"Jana","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8901-2531","authenticated-orcid":true,"given":"Nilkamal","family":"Mahanta","sequence":"additional","affiliation":[{"name":"Department of Chemistry","place":["Dharwad, India"]},{"name":"Indian Institute of Technology Dharwad","place":["Dharwad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.3390\/v13040667"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01157"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1632"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2021.02.001"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4010"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1039\/B816215H"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b00325"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.31.519"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1021\/ja00483a047"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.31.511"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.38.415"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-009-0218-9"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1039\/D0NP00064G"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202300342"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1094\/PD-78-0420"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1016\/0261-2194(95)00129-8"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-81-84"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1016\/S0261-2194(99)00048-4"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2006.12.002"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2003.10.001"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-005-6561-6"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800008"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201200173"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1021\/bi400316p"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku486"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1038\/srep39675"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1007\/s11434-012-5554-8"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.110605"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01654-24"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b01016"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b00130"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.8b00039"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201903500"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1039\/C8NP00049B"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-020-02885-6"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01172-14"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080010210"},{"key":"ref38\/cit38","unstructured":"Green, M. R.; Sambrook, J.; Sambrook, J.\n                      Molecular Cloning: A Laboratory Manual\n                      , 4th ed. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y., 2012; p 978."},{"issue":"4","key":"ref39\/cit39","first-page":"561","volume":"6","author":"Singh M.","year":"2010","journal-title":"J. Biotechnol. Biochem."},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-1177(02)00057-7"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.05.022"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21303"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1021\/ci200227u"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20945"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21287"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.5b00935"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.3c01153"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.24829"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh381"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm276"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21720"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3280"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4067"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.1021\/j100059a038"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1063\/1.445869"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21367"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevA.33.3628"},{"key":"ref61\/cit61","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2003.12.039"},{"key":"ref62\/cit62","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9991(77)90098-5"},{"key":"ref63\/cit63","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1600823"},{"key":"ref64\/cit64","doi-asserted-by":"publisher","DOI":"10.1038\/nature14473"},{"key":"ref65\/cit65","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15447"},{"key":"ref66\/cit66","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/9780470110362.ch1","volume-title":"Methods of Biochemical Analysis","volume":"18","author":"Andrew P.","year":"1970"},{"key":"ref67\/cit67","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2230"},{"key":"ref68\/cit68","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15277"},{"key":"ref69\/cit69","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-013-1508-y"},{"key":"ref70\/cit70","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900351"},{"key":"ref71\/cit71","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2009.11.005"},{"key":"ref72\/cit72","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.5b00407"},{"key":"ref73\/cit73","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/B978-0-12-378630-2.00127-4","volume-title":"Encyclopedia of Biological Chemistry","author":"Hayaishi O.","year":"2013"},{"key":"ref74\/cit74","first-page":"447","volume-title":"Methods Enzymol.","volume":"486","author":"Pacheco A. A.","year":"2011"},{"key":"ref75\/cit75","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref76\/cit76","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"ref77\/cit77","doi-asserted-by":"publisher","DOI":"10.1021\/ct400341p"},{"key":"ref78\/cit78","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002708"},{"key":"ref79\/cit79","doi-asserted-by":"publisher","DOI":"10.1109\/TCBB.2015.2459680"},{"key":"ref80\/cit80","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2019.07.007"},{"key":"ref81\/cit81","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.20042"},{"key":"ref82\/cit82","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2012.08468.x"},{"key":"ref83\/cit83","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90691-C"},{"key":"ref84\/cit84","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a124509"},{"key":"ref85\/cit85","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29846-0"},{"key":"ref86\/cit86","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3594"},{"key":"ref87\/cit87","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.375238"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.6c00271","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.6c00271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T08:10:21Z","timestamp":1780387821000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.biochem.6c00271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":87,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6,2]]}},"alternative-id":["10.1021\/acs.biochem.6c00271"],"URL":"https:\/\/doi.org\/10.1021\/acs.biochem.6c00271","relation":{"has-preprint":[{"id-type":"doi","id":"10.64898\/2026.02.25.707955","asserted-by":"object"}]},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]}}}