{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:25:59Z","timestamp":1787059559054,"version":"build-2736575974"},"reference-count":52,"publisher":"American Chemical Society (ACS)","issue":"16","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["1S10OD016305"],"award-info":[{"award-number":["1S10OD016305"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM117212"],"award-info":[{"award-number":["R01GM117212"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM156806"],"award-info":[{"award-number":["R35GM156806"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The interaction between mammalian enabled protein (Mena), an actin regulatory protein, and protein tyrosine phosphatase 1B (PTP1B) is critical for maintaining epidermal growth factor receptor (EGFR) signaling homeostasis and regulating cellular motility. Mena recruits PTP1B to activated EGFR in vivo, facilitating receptor dephosphorylation and limiting invasive signaling. However, overexpression of wild-type Mena or the invasive isoform, MenaINV, perturbs this regulatory mechanism by altering PTP1B localization and EGFR signaling dynamics. Here, we report the solution NMR structure of the unliganded Mena EVH1 domain, which contains a conserved aromatic triad (Y16, W23, F77) that forms the canonical polyproline-binding cleft. Building on prior evidence that the PTP1B polyproline region serves as an EVH1 ligand, we investigated the structural basis of the interaction between EVH1 and wild-type PTP1B. NMR chemical shift perturbation analyses using multiple PTP1B variants, including full-length and truncated constructs, revealed that binding affects not only the canonical EVH1 hydrophobic cleft but also residues on the opposite surface, suggesting an expanded interaction interface. Diffusion-ordered spectroscopy (DOSY) and size-exclusion chromatography further indicate that PTP1B variants containing the disordered C-terminal tail form more compact complexes with EVH1, consistent with a disorder-to-order transition. Complementary perturbation studies using [2H,15N] PTP1B demonstrate that EVH1 binding induces changes not only in the polyproline region but also across the phosphatase core, \u03b17 helix, and C-terminal tail. Despite these structural perturbations, enzymatic assays show that EVH1 binding does not affect the catalytic activity of PTP1B, supporting a model in which Mena functions as a scaffold to spatially organize PTP1B within EGFR signaling complexes.<\/jats:p>","DOI":"10.1021\/acs.biochem.6c00299","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T04:01:59Z","timestamp":1786161719000},"page":"2470-2484","source":"Crossref","is-referenced-by-count":0,"title":["Mena EVH1 Interacts\nwith PTP1B Via a Distributed Interface\nWithout Regulating Enzymatic Activity"],"prefix":"10.1021","volume":"65","author":[{"given":"Lanette","family":"LaComb","sequence":"first","affiliation":[{"name":"Albert Einstein College of Medicine , , 1300 Morris Park Avenue , ,","place":["Bronx, NY, 10461"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2877-0704","authenticated-orcid":true,"given":"Sean M.","family":"Cahill","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine , , 1300 Morris Park Avenue , ,","place":["Bronx, NY, 10461"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey B.","family":"Bonanno","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine , , 1300 Morris Park Avenue , ,","place":["Bronx, NY, 10461"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6770-7172","authenticated-orcid":true,"given":"David","family":"Cowburn","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine , , 1300 Morris Park Avenue , ,","place":["Bronx, NY, 10461"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven C.","family":"Almo","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine , , 1300 Morris Park Avenue , ,","place":["Bronx, NY, 10461"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7753-0240","authenticated-orcid":true,"given":"Agnidipta","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine , , 1300 Morris Park Avenue , ,","place":["Bronx, NY, 10461"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"2026081808123562500_cit1","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1146\/annurev.cellbio.19.050103.103356","article-title":"Ena\/VASP\nproteins: regulators of the actin cytoskeleton and cell migration","volume":"19","author":"Krause","year":"2003","journal-title":"Annu. Rev. Cell Dev. Biol."},{"issue":"18","key":"2026081808123562500_cit2","doi-asserted-by":"publisher","first-page":"4903","DOI":"10.1093\/emboj\/19.18.4903","article-title":"Dual epitope recognition\nby the VASP EVH1 domain modulates polyproline ligand specificity and\nbinding affinity","volume":"19","author":"Ball","year":"2000","journal-title":"EMBO J."},{"issue":"2","key":"2026081808123562500_cit3","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/s0092-8674(00)81341-0","article-title":"a relative\nof VASP and Drosophila Enabled, is implicated in the control of microfilament\ndynamics","volume":"87","author":"Gertler","year":"1996","journal-title":"Cell"},{"issue":"21","key":"2026081808123562500_cit4","doi-asserted-by":"publisher","first-page":"3867","DOI":"10.1091\/mbc.E15-06-0442","article-title":"PTP1B-dependent regulation\nof receptor tyrosine kinase\nsignaling by the actin-binding protein Mena","volume":"26","author":"Hughes","year":"2015","journal-title":"Mol. Biol. Cell"},{"issue":"1","key":"2026081808123562500_cit5","doi-asserted-by":"publisher","first-page":"36142","DOI":"10.1038\/srep36142","article-title":"MenaINV dysregulates cortactin phosphorylation\nto promote invadopodium maturation","volume":"6","author":"Weidmann","year":"2016","journal-title":"Sci. Rep."},{"issue":"14","key":"2026081808123562500_cit6","doi-asserted-by":"publisher","first-page":"6731","DOI":"10.1016\/S0021-9258(18)68703-4","article-title":"Characterization of the major protein-tyrosine-phosphatases\nof human placenta","volume":"263","author":"Tonks","year":"1988","journal-title":"J. Biol. Chem."},{"issue":"13","key":"2026081808123562500_cit7","doi-asserted-by":"publisher","first-page":"7027","DOI":"10.3390\/ijms23137027","article-title":"Human Protein\nTyrosine Phosphatase 1B (PTP1B): From\nStructure to Clinical Inhibitor Perspectives","volume":"23","author":"Liu","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"5","key":"2026081808123562500_cit8","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1080\/07391102.2011.10508599","article-title":"Alpha7 helix plays\nan important role in the conformational\nstability of PTP1B","volume":"28","author":"Olmez","year":"2011","journal-title":"J. Biomol. Struct. Dyn."},{"issue":"17","key":"2026081808123562500_cit9","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.1021\/acs.biochem.4c00331","article-title":"Insights\ninto the Interaction Landscape of the EVH1\nDomain of Mena","volume":"63","author":"LaComb","year":"2024","journal-title":"Biochemistry"},{"issue":"3","key":"2026081808123562500_cit10","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/0092-8674(92)90190-N","article-title":"The nontransmembrane\ntyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum\nvia its 35 amino acid C-terminal sequence","volume":"68","author":"Frangioni","year":"1992","journal-title":"Cell"},{"issue":"12","key":"2026081808123562500_cit11","doi-asserted-by":"publisher","first-page":"4843","DOI":"10.1002\/j.1460-2075.1993.tb06174.x","article-title":"Calpain-catalyzed\ncleavage and subcellular relocation of protein phosphotyrosine phosphatase\n1B (PTP-1B) in human platelets","volume":"12","author":"Frangioni","year":"1993","journal-title":"EMBO J."},{"issue":"5","key":"2026081808123562500_cit12","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1021\/bi00379a035","article-title":"Epidermal\ngrowth factor induces rapid, reversible aggregation\nof the purified epidermal growth factor receptor","volume":"26","author":"Yarden","year":"1987","journal-title":"Biochemistry"},{"issue":"3","key":"2026081808123562500_cit13","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1007\/s10585-015-9775-5","article-title":"Characterization of the expression of the pro-metastatic\nMenaINV isoform during breast tumor progression","volume":"33","author":"Oudin","year":"2016","journal-title":"Clin. Exp. Metastasis"},{"issue":"353","key":"2026081808123562500_cit14","doi-asserted-by":"publisher","first-page":"ra112","DOI":"10.1126\/scisignal.2005329","article-title":"Invasive breast carcinoma\ncells from patients\nexhibit Mena INV - and macrophage-dependent transendothelial\nmigration","volume":"7","author":"Pignatelli","year":"2014","journal-title":"Sci. Signaling"},{"issue":"6","key":"2026081808123562500_cit15","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1007\/s10585-011-9388-6","article-title":"Mena invasive (MenaINV)\nand Mena11a isoforms\nplay distinct roles in breast cancer cell cohesion and association\nwith TMEM","volume":"28","author":"Roussos","year":"2011","journal-title":"Clin. Exp. Metastasis"},{"issue":"7","key":"2026081808123562500_cit16","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1038\/nchembio.1528","article-title":"Targeting the disordered C terminus of PTP1B\nwith an allosteric inhibitor","volume":"10","author":"Krishnan","year":"2014","journal-title":"Nat. Chem. Biol."},{"issue":"1","key":"2026081808123562500_cit17","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0014-5793(01)03243-4","article-title":"Pleckstrin\nhomology domains and the\ncytoskeleton","volume":"513","author":"Lemmon","year":"2002","journal-title":"FEBS Lett."},{"issue":"4","key":"2026081808123562500_cit18","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1002\/jcb.240560403","article-title":"Pleckstrin homology\n(PH) domains in signal transduction","volume":"56","author":"Ingley","year":"1994","journal-title":"J. Cell.\nBiochem."},{"issue":"6","key":"2026081808123562500_cit19","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1016\/0092-8674(95)90219-8","article-title":"Structure of the\nhigh affinity complex of inositol trisphosphate with a phospholipase\nC pleckstrin homology domain","volume":"83","author":"Ferguson","year":"1995","journal-title":"Cell"},{"issue":"4","key":"2026081808123562500_cit20","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/S0092-8674(00)80757-6","article-title":"Structure\nof the enabled\/VASP homology\n1 domain-peptide complex: a key component in the spatial control of\nactin assembly","volume":"97","author":"Prehoda","year":"1999","journal-title":"Cell"},{"key":"2026081808123562500_cit21","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1146\/annurev.biophys.27.1.503","article-title":"Pleckstrin homology\ndomains: a common fold with diverse\nfunctions","volume":"27","author":"Rebecchi","year":"1998","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"issue":"1","key":"2026081808123562500_cit22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1042\/bj3500001","article-title":"Signal-dependent\nmembrane targeting by pleckstrin homology\n(PH) domains","volume":"350","author":"Lemmon","year":"2000","journal-title":"Biochem. J."},{"issue":"1","key":"2026081808123562500_cit23","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0896-6273(00)81145-9","article-title":"Structure of the Homer EVH1 domain-peptide\ncomplex reveals a new twist in polyproline recognition","volume":"26","author":"Beneken","year":"2000","journal-title":"Neuron"},{"issue":"1","key":"2026081808123562500_cit24","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/S0014-5793(01)03291-4","article-title":"EVH1 domains: structure,\nfunction and interactions","volume":"513","author":"Ball","year":"2002","journal-title":"FEBS Lett."},{"issue":"21","key":"2026081808123562500_cit25","doi-asserted-by":"publisher","first-page":"4597","DOI":"10.1038\/sj.emboj.7601874","article-title":"Structural\nbasis for the recruitment of profilin-actin complexes during filament\nelongation by Ena\/VASP","volume":"26","author":"Ferron","year":"2007","journal-title":"EMBO J."},{"issue":"14","key":"2026081808123562500_cit26","doi-asserted-by":"publisher","first-page":"833","DOI":"10.2741\/3281","article-title":"Diversity\nof polyproline recognition by EVH1 domains","volume":"Volume","author":"Peterson","year":"2009","journal-title":"Front. Biosci."},{"issue":"47","key":"2026081808123562500_cit27","doi-asserted-by":"publisher","first-page":"29684","DOI":"10.1073\/pnas.2007213117","article-title":"Designed nanomolar small-molecule inhibitors\nof Ena\/VASP EVH1 interaction impair invasion and extravasation of\nbreast cancer cells","volume":"117","author":"Barone","year":"2020","journal-title":"Proc. Natl. Acad. Sci.\nU. S. A."},{"key":"2026081808123562500_cit28","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.70601","article-title":"A distributed residue network permits\nconformational binding specificity in a conserved family of actin\nremodelers","volume":"10","author":"Hwang","year":"2021","journal-title":"eLife"},{"issue":"4","key":"2026081808123562500_cit29","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/S0092-8674(00)80757-6","article-title":"Structure of the Enabled\/VASP Homology\n1 Domain\u2013Peptide Complex","volume":"97","author":"Prehoda","year":"1999","journal-title":"Cell"},{"issue":"7873","key":"2026081808123562500_cit30","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction\nwith AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"6","key":"2026081808123562500_cit31","doi-asserted-by":"publisher","first-page":"151098","DOI":"10.1016\/j.ejcb.2020.151098","article-title":"The role of the tumor microenvironment\nin tumor cell intravasation and dissemination","volume":"99","author":"Borriello","year":"2020","journal-title":"Eur. J. Cell Biol."},{"issue":"1","key":"2026081808123562500_cit32","doi-asserted-by":"publisher","DOI":"10.1002\/pro.70016","article-title":"Allosteric regulation\nof the tyrosine\nphosphatase PTP1B by a protein-protein interaction","volume":"34","author":"Chartier","year":"2025","journal-title":"Protein Sci."},{"issue":"1","key":"2026081808123562500_cit33","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1038\/s41467-021-27633-6","article-title":"The catalytic activity of TCPTP is auto-regulated by\nits intrinsically disordered tail and activated by Integrin alpha-1","volume":"13","author":"Singh","year":"2022","journal-title":"Nat. Commun."},{"issue":"2","key":"2026081808123562500_cit34","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1021\/bc200562y","article-title":"Specific inhibition\nof sensitized protein tyrosine\nphosphatase 1B (PTP1B) with a biarsenical probe","volume":"23","author":"Davis","year":"2012","journal-title":"Bioconjug. Chem."},{"issue":"6","key":"2026081808123562500_cit35","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1016\/j.devcel.2008.09.003","article-title":"A Mena invasion isoform\npotentiates EGF-induced carcinoma cell invasion and metastasis","volume":"15","author":"Philippar","year":"2008","journal-title":"Dev. Cell"},{"issue":"3","key":"2026081808123562500_cit36","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1091\/mbc.e05-07-0644","article-title":"Ena\/VASP proteins\ncan regulate distinct modes of actin\norganization at cadherin-adhesive contacts","volume":"17","author":"Scott","year":"2006","journal-title":"Mol. Biol. Cell"},{"issue":"5","key":"2026081808123562500_cit37","doi-asserted-by":"publisher","first-page":"H1841","DOI":"10.1152\/ajpheart.01127.2010","article-title":"Mammalian enabled (Mena) is a critical\nregulator of cardiac function","volume":"300","author":"Aguilar","year":"2011","journal-title":"Am. J. Physiol.\nHeart Circ. Physiol."},{"issue":"13","key":"2026081808123562500_cit38","doi-asserted-by":"publisher","first-page":"2120","DOI":"10.1242\/jcs.086231","article-title":"Mena invasive (MenaINV)\npromotes multicellular\nstreaming motility and transendothelial migration in a mouse model\nof breast cancer","volume":"124","author":"Roussos","year":"2011","journal-title":"J. Cell Sci."},{"issue":"6","key":"2026081808123562500_cit39","doi-asserted-by":"publisher","first-page":"R101","DOI":"10.1186\/bcr2784","article-title":"Mena deficiency\ndelays tumor progression and decreases metastasis in polyoma middle-T\ntransgenic mouse mammary tumors","volume":"12","author":"Roussos","year":"2010","journal-title":"Breast Cancer\nRes."},{"issue":"5","key":"2026081808123562500_cit40","doi-asserted-by":"publisher","first-page":"1470","DOI":"10.1158\/1078-0432.CCR-05-2027","article-title":"The cytoskeleton regulatory protein hMena (ENAH) is overexpressed\nin human benign breast lesions with high risk of transformation and\nhuman epidermal growth factor receptor-2-positive\/hormonal receptor-negative\ntumors","volume":"12","author":"Di Modugno","year":"2006","journal-title":"Clin. Cancer Res."},{"issue":"12","key":"2026081808123562500_cit41","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.1242\/jcs.038125","article-title":"Ena\/VASP: towards\nresolving a pointed controversy at\nthe barbed end","volume":"122","author":"Bear","year":"2009","journal-title":"J. Cell Sci."},{"issue":"5","key":"2026081808123562500_cit42","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1158\/2159-8290.CD-16-0231","article-title":"MENA Promotes Tumor-Intrinsic Metastasis through ECM Remodeling and\nHaptotaxis","volume":"6","author":"Santiago-Medina","year":"2016","journal-title":"Cancer Discov"},{"issue":"11","key":"2026081808123562500_cit43","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1038\/nrm2039","article-title":"Protein\ntyrosine phosphatases: from genes, to function, to disease","volume":"7","author":"Tonks","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"1","key":"2026081808123562500_cit44","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1021\/acs.accounts.6b00537","article-title":"Drugging\nthe Undruggable: Therapeutic Potential of Targeting Protein Tyrosine\nPhosphatases","volume":"50","author":"Zhang","year":"2017","journal-title":"Acc. Chem. Res."},{"issue":"10","key":"2026081808123562500_cit45","doi-asserted-by":"publisher","first-page":"1563","DOI":"10.4155\/fmc.10.241","article-title":"Protein\ntyrosine phosphatases as drug targets: strategies and challenges of\ninhibitor development","volume":"2","author":"Barr","year":"2010","journal-title":"Future Med. Chem."},{"issue":"2","key":"2026081808123562500_cit46","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1517\/13543784.12.2.223","article-title":"PTP1B inhibitors\nas potential therapeutics in the treatment\nof type 2 diabetes and obesity","volume":"12","author":"Zhang","year":"2003","journal-title":"Expert Opin.\nInvest. Drugs"},{"issue":"42","key":"2026081808123562500_cit47","doi-asserted-by":"publisher","first-page":"8427","DOI":"10.1021\/acs.jpcb.2c05423","article-title":"Allosteric Inhibition\nof PTP1B by a Nonpolar Terpenoid","volume":"126","author":"Friedman","year":"2022","journal-title":"J. Phys. Chem.\nB"},{"issue":"3","key":"2026081808123562500_cit48","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1016\/j.bbapap.2009.09.018","article-title":"The two\nfaces of PTP1B in cancer","volume":"1804","author":"Lessard","year":"2010","journal-title":"Biochim. Biophys.\nActa Gen. Subj."},{"issue":"4","key":"2026081808123562500_cit49","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1038\/nsb0497-298","article-title":"The kinetic folding intermediate\nof ribonuclease H\nresembles the acid molten globule and partially unfolded molecules\ndetected under native conditions","volume":"4","author":"Raschke","year":"1997","journal-title":"Nat. Struct.\nBiol."},{"key":"2026081808123562500_cit50","doi-asserted-by":"publisher","first-page":"015","DOI":"10.1385\/1-59259-762-9:015","article-title":"Quantitative\nanalysis of protein-protein interactions","volume":"261","author":"Wilkinson","year":"2004","journal-title":"Methods\nMol. Biol."},{"issue":"9","key":"2026081808123562500_cit51","doi-asserted-by":"publisher","first-page":"4108","DOI":"10.1021\/cr900033p","article-title":"Theory, Practice, and\nApplications of Paramagnetic\nRelaxation Enhancement for the Characterization of Transient Low-Population\nStates of Biological Macromolecules and Their Complexes","volume":"109","author":"Clore","year":"2009","journal-title":"Chem. Rev."},{"issue":"1","key":"2026081808123562500_cit52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1021\/bi011870b","article-title":"Mapping\nProtein\u2013Protein Interactions in Solution by NMR Spectroscopy","volume":"41","author":"Zuiderweg","year":"2002","journal-title":"Biochemistry"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.6c00299","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/bichaw\/article-pdf\/65\/16\/2470\/66463583\/acs.biochem.6c00299.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/bichaw\/article-pdf\/65\/16\/2470\/66463583\/acs.biochem.6c00299.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:41:43Z","timestamp":1787056903000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/bichaw\/article\/65\/16\/2470\/5250614\/Mena-EVH1-Interacts-with-PTP1B-Via-a-Distributed"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,7]]},"references-count":52,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8,7]]},"published-print":{"date-parts":[[2026,8,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.biochem.6c00299","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,7]]}}}