{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T11:10:40Z","timestamp":1774955440961,"version":"3.50.1"},"reference-count":63,"publisher":"American Chemical Society (ACS)","issue":"31","funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI110794"],"award-info":[{"award-number":["R01 AI110794"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2018,8,7]]},"DOI":"10.1021\/acs.biochem.8b00419","type":"journal-article","created":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T15:07:11Z","timestamp":1530284831000},"page":"4726-4734","source":"Crossref","is-referenced-by-count":4,"title":["A Yeast Three Hybrid Assay for Metabolic Engineering of Tetracycline Derivatives"],"prefix":"10.1021","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5570-5804","authenticated-orcid":true,"given":"Ehud","family":"Herbst","sequence":"first","affiliation":[{"name":"Department of Chemistry, Columbia University, New York, New York 10027, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro A.","family":"Baldera-Aguayo","sequence":"additional","affiliation":[{"name":"Integrated Program in Cellular, Molecular and Biomedical Studies, Columbia University, New York, New York 10032, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunwook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Columbia University, New York, New York 10027, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia W.","family":"Cornish","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Columbia University, New York, New York 10027, United States"},{"name":"Department of Systems Biology, Columbia University, New York, New York 10032, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2018,6,29]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2017.09.002"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1193990"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1111\/1567-1364.12171"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2918"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.02.011"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf4557"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1038\/nature08187"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258096"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2016.03.005"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2014.01.005"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10606"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.12817"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.10.4604"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb2001s82"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(02)00015-4"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)00884-5"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.262420099"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1038\/340245a0"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1104"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1002\/0471143030.cb0106s04"},{"key":"ref22\/cit22","unstructured":"Krishnan, L., Sum, P.E., Daigneault, S., Bernatchez, M., Pilcher, A. S., Horne, J. M., Tuper, A. J., Mccauley, III, J. J., and Michaud, A. P. (2006) Methods of purifying tigecycline, U.S. Patent 2007\/0049561 A1, March 1, 2017."},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.13.6373"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2004.11.049"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.06.035"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911566106"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1002\/1439-7633(20020902)3:9<887::AID-CBIC887>3.0.CO;2-F"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201300186"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.087080"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1021\/ja206906d"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1603221"},{"key":"ref32\/cit32","volume-title":"International Conference on Harmonization","author":"ICH Expert Working Group.","year":"2005"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00329-0"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/350250a0"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1021\/ja9941532"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90498-F"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1021\/bi952683e"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153629"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1038\/73324"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.17.6089"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7497-7510.2004"},{"key":"ref42\/cit42","first-page":"1","volume":"2","author":"Brow M. A. D.","year":"1985","journal-title":"Mol. Biol. Evol."},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.24.9415"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1007\/BF00331306"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147363"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00043-12"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.12.2699"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2978-2980.2003"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(80)90101-8"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.48.100194.002021"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.7.4708"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.0138"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1080\/08998280.2006.11928154"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.03.011"},{"key":"ref55\/cit55","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/978-3-642-70304-1_5","volume-title":"The Tetracyclines","author":"Rogalski W.","year":"1985"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.19.11181"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bms190"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.7b00602"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5547"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00427-3"},{"key":"ref61\/cit61","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.69.2.326-356.2005"},{"key":"ref62\/cit62","doi-asserted-by":"publisher","DOI":"10.1021\/ja046238v"},{"key":"ref63\/cit63","doi-asserted-by":"publisher","DOI":"10.1021\/ja8055744"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biochem.8b00419","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T00:48:51Z","timestamp":1682124531000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.biochem.8b00419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,29]]},"references-count":63,"journal-issue":{"issue":"31","published-print":{"date-parts":[[2018,8,7]]}},"alternative-id":["10.1021\/acs.biochem.8b00419"],"URL":"https:\/\/doi.org\/10.1021\/acs.biochem.8b00419","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,29]]}}}