{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T14:17:02Z","timestamp":1793888222719,"version":"4.8.0"},"reference-count":61,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/www.nationalarchives.gov.uk\/doc\/open-government-licence\/version\/3\/"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["1S10OD010645-01A1"],"award-info":[{"award-number":["1S10OD010645-01A1"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"UK Ministry of Defence","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Melioidosis is an emerging infectious disease caused by Burkholderia pseudomallei and is associated with high morbidity and mortality rates in endemic areas. Antibiotic treatment is protracted and not always successful; even with appropriate therapy, up to 40% of individuals presenting with melioidosis in Thailand succumb to infection. In these circumstances, an effective vaccine has the potential to have a dramatic impact on both the scale and the severity of disease. Currently, no vaccines are licensed for human use. A leading vaccine candidate is the capsular polysaccharide consisting of a homopolymer of unbranched 1\u21923 linked 2-O-acetyl-6-deoxy-\u03b2-d-manno-heptopyranose. Here, we present the chemical synthesis of this challenging antigen using a novel modular disaccharide assembly approach. The resulting hexasaccharide was coupled to the nontoxic Hc domain of tetanus toxin as a carrier protein to promote recruitment of T-cell help and provide a scaffold for antigen display. Mice immunized with the glycoconjugate developed IgM and IgG responses capable of recognizing native capsule, and were protected against infection with over 120 \u00d7 LD50 of B. pseudomallei strain K96243. This is the first report of the chemical synthesis of an immunologically relevant and protective hexasaccharide fragment of the capsular polysaccharide of B. pseudomallei and serves as the rational starting point for the development of an effective licensed vaccine for this emerging infectious disease.<\/jats:p>","DOI":"10.1021\/acs.bioconjchem.5b00525","type":"journal-article","created":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T17:30:22Z","timestamp":1461864622000},"page":"1435-1446","source":"Crossref","is-referenced-by-count":41,"title":["Protection against Experimental Melioidosis with a\nSynthetic\n                    <i>manno<\/i>\n                    -Heptopyranose Hexasaccharide Glycoconjugate"],"prefix":"10.1021","volume":"27","author":[{"given":"Andrew E.","family":"Scott","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory , Porton Down , , ,","place":["Salisbury, Wiltshire, United Kingdom, SP4 0JQ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Christ","sequence":"additional","affiliation":[{"name":"Corden Pharma International Inc. (formerly Ancora Pharmaceuticals Inc.) , ,","place":["Woburn, Massachusetts, United States, 01801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison J.","family":"George","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory , Porton Down , , ,","place":["Salisbury, Wiltshire, United Kingdom, SP4 0JQ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret G. M.","family":"Stokes","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory , Porton Down , , ,","place":["Salisbury, Wiltshire, United Kingdom, SP4 0JQ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J. S.","family":"Lohman","sequence":"additional","affiliation":[{"name":"Corden Pharma International Inc. (formerly Ancora Pharmaceuticals Inc.) , ,","place":["Woburn, Massachusetts, United States, 01801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Guo","sequence":"additional","affiliation":[{"name":"Corden Pharma International Inc. (formerly Ancora Pharmaceuticals Inc.) , ,","place":["Woburn, Massachusetts, United States, 01801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Jones","sequence":"additional","affiliation":[{"name":"Corden Pharma International Inc. (formerly Ancora Pharmaceuticals Inc.) , ,","place":["Woburn, Massachusetts, United States, 01801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[{"name":"University of Exeter , , , ,","place":["Exeter, Devon, United Kingdom, EX4 4QD"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Atkins","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory , Porton Down , , ,","place":["Salisbury, Wiltshire, United Kingdom, SP4 0JQ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Stewart","family":"Campbell","sequence":"additional","affiliation":[{"name":"Corden Pharma International Inc. (formerly Ancora Pharmaceuticals Inc.) , ,","place":["Woburn, Massachusetts, United States, 01801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joann L.","family":"Prior","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory , Porton Down , , ,","place":["Salisbury, Wiltshire, United Kingdom, SP4 0JQ"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2016,4,28]]},"reference":[{"key":"2026090213094340400_cit1","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1056\/NEJMra1204699","article-title":"Melioidosis","volume":"367","author":"Wiersinga","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"2026090213094340400_cit2","doi-asserted-by":"publisher","first-page":"e2105","DOI":"10.1371\/journal.pntd.0002105","article-title":"Systematic Review and Consensus Guidelines\nfor Environmental Sampling of Burkholderia pseudomallei","volume":"7","author":"Limmathurotsakul","year":"2013","journal-title":"PLoS Neglected Trop. Dis."},{"key":"2026090213094340400_cit3","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1093\/cid\/cir808","article-title":"Clinical\nFeatures\nand Epidemiology of Melioidosis Pneumonia: Results From a 21-Year\nStudy and Review of the Literature","volume":"54","author":"Meumann","year":"2012","journal-title":"Clin. Infect.\nDis."},{"key":"2026090213094340400_cit4","doi-asserted-by":"publisher","first-page":"e900","DOI":"10.1371\/journal.pntd.0000900","article-title":"The Epidemiology\nand Clinical Spectrum\nof Melioidosis: 540 Cases from the 20 Year Darwin Prospective Study","volume":"4","author":"Currie","year":"2010","journal-title":"PLoS Neglected Trop. Dis."},{"key":"2026090213094340400_cit5","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.ijantimicag.2014.01.005","article-title":"Treatment\nand prophylaxis of melioidosis","volume":"43","author":"Dance","year":"2014","journal-title":"Int. J. Antimicrob.\nAgents"},{"key":"2026090213094340400_cit6","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1086\/318116","article-title":"Endemic Melioidosis in Tropical Northern Australia:\nA 10-Year Prospective Study and Review of the Literature","volume":"31","author":"Currie","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"2026090213094340400_cit7","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.4269\/ajtmh.2010.10-0038","article-title":"Increasing Incidence of Human Melioidosis\nin Northeast Thailand","volume":"82","author":"Limmathurotsakul","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2026090213094340400_cit8","doi-asserted-by":"publisher","first-page":"198","DOI":"10.3389\/fmicb.2011.00198","article-title":"Development of vaccines\nagainst Burkholderia pseudomallei","volume":"2","author":"Patel","year":"2011","journal-title":"Front. Microbiol."},{"key":"2026090213094340400_cit9","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00005","article-title":"Burkholderia vaccines: are we moving forward?","volume":"3","author":"Choh","year":"2013","journal-title":"Front.\nCell. Infect. Microbiol."},{"key":"2026090213094340400_cit10","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00010","article-title":"Development of Burkholderia mallei and pseudomallei vaccines","volume":"3","author":"Silva","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"2026090213094340400_cit11","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S0008-6215(00)90930-3","article-title":"Structure\nof the polysaccharide chains of Pseudomonas pseudomallei lipopolysaccharides","volume":"233","author":"Knirel","year":"1992","journal-title":"Carbohydr. Res."},{"key":"2026090213094340400_cit12","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1128\/iai.63.9.3348-3352.1995","article-title":"Structural\ncharacterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei","volume":"63","author":"Perry","year":"1995","journal-title":"Infect. Immun."},{"key":"2026090213094340400_cit13","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1128\/IAI.69.1.34-44.2001","article-title":"Detection of Bacterial\nVirulence Genes by Subtractive Hybridization: Identification of Capsular\nPolysaccharide of Burkholderia pseudomallei as a\nMajor Virulence Determinant","volume":"69","author":"Reckseidler","year":"2001","journal-title":"Infect. Immun."},{"key":"2026090213094340400_cit14","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1099\/0022-1317-51-7-539","article-title":"Characterisation of\nan acapsular mutant of Burkholderia pseudomallei identified\nby signature tagged\nmutagenesis","volume":"51","author":"Atkins","year":"2002","journal-title":"J. Med. Microbiol."},{"key":"2026090213094340400_cit15","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1006\/mpat.2000.0430","article-title":"Identification of\na Burkholderia mallei polysaccharide gene cluster\nby subtractive hybridization and demonstration that the encoded capsule\nis an essential virulence determinant","volume":"30","author":"DeShazer","year":"2001","journal-title":"Microb.\nPathog."},{"key":"2026090213094340400_cit16","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1099\/jmm.0.45766-0","article-title":"Evaluation of lipopolysaccharide and capsular polysaccharide\nas subunit vaccines against experimental melioidosis","volume":"53","author":"Nelson","year":"2004","journal-title":"J. Med. Microbiol."},{"key":"2026090213094340400_cit17","doi-asserted-by":"publisher","first-page":"3206","DOI":"10.1128\/IAI.01847-14","article-title":"Burkholderia pseudomallei capsular polysaccharide conjugates provide protection\nagainst acute melioidosis","volume":"82","author":"Scott","year":"2014","journal-title":"Infect. Immun."},{"key":"2026090213094340400_cit18","doi-asserted-by":"publisher","first-page":"108","DOI":"10.3389\/fcimb.2012.00108","article-title":"Development of capsular\npolysaccharide-based\nglycoconjugates for immunization against melioidosis and glanders","volume":"2","author":"Burtnick","year":"2012","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"2026090213094340400_cit19","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.carres.2011.12.011","article-title":"Structural\nanalysis of capsular polysaccharides expressed by Burkholderia mallei and Burkholderia pseudomallei","volume":"349","author":"Heiss","year":"2012","journal-title":"Carbohydr. Res."},{"key":"2026090213094340400_cit20","first-page":"141","article-title":"Influence of Protecting Groups on the Reactivity and Selectivity\nof Glycosylation: Chemistry of the 4,6-O-Benzylidene Protected Mannopyranosyl\nDonors and Related Species","volume-title":"Reactivity\nTuning in Oligosaccharide Assembly","author":"Aubry","year":"2011"},{"key":"2026090213094340400_cit21","doi-asserted-by":"publisher","first-page":"13113","DOI":"10.1021\/ja405990z","article-title":"Chemical Synthesis of\nHighly Congested gp120 V1V2 N-Glycopeptide Antigens for Potential\nHIV-1-Directed Vaccines","volume":"135","author":"Aussedat","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"2026090213094340400_cit22","doi-asserted-by":"publisher","first-page":"13283","DOI":"10.1039\/C5CC04716A","article-title":"\u03b2-Mannosylation\nwith 4, 6-benzylidene protected mannosyl donors\nwithout preactivation","volume":"51","author":"Heuckendorff","year":"2015","journal-title":"Chem. Commun."},{"key":"2026090213094340400_cit23","doi-asserted-by":"publisher","first-page":"2295","DOI":"10.1021\/acschembio.5b00502","article-title":"Burkholderia pseudomallei capsular\npolysaccharide recognition by a monoclonal antibody reveals key details\ntoward a biodefense vaccine and diagnostics against melioidosis","volume":"10","author":"Marchetti","year":"2015","journal-title":"ACS Chem. Biol."},{"key":"2026090213094340400_cit24","doi-asserted-by":"publisher","first-page":"4615","DOI":"10.1021\/jo500640n","article-title":"Intramolecular Aglycon\nDelivery Enables the Synthesis of 6-Deoxy-\u03b2-d-manno-heptosides as Fragments of Burkholderia pseudomallei and Burkholderia mallei Capsular Polysaccharide","volume":"79","author":"Tamigney Kenfack","year":"2014","journal-title":"J. Org. Chem."},{"key":"2026090213094340400_cit25","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4049\/jimmunol.84.1.82","article-title":"The Upper\nLimit for the Size of the Human Antidextran Combining Site","volume":"84","author":"Kabat","year":"1960","journal-title":"J. Immunol."},{"key":"2026090213094340400_cit26","doi-asserted-by":"publisher","first-page":"126","DOI":"10.2174\/156802608783378864","article-title":"Recent Developments\nin Synthetic Oligosaccharide-Based Bacterial Vaccines","volume":"8","author":"Pozsgay","year":"2008","journal-title":"Curr. Top. Med. Chem."},{"key":"2026090213094340400_cit27","doi-asserted-by":"publisher","first-page":"9376","DOI":"10.1021\/ja00024a057","article-title":"Synthesis of \u03b2-mannopyranosides\nby intramolecular\naglycon delivery","volume":"113","author":"Barresi","year":"1991","journal-title":"J. Am. Chem. Soc."},{"key":"2026090213094340400_cit28","doi-asserted-by":"publisher","first-page":"2945","DOI":"10.1016\/j.tetlet.2014.03.099","article-title":"Intramolecular aglycon delivery for\n(1\u2192 2)-\u03b2-mannosylation: towards the synthesis of phospholipomannan\nof Candida albicans","volume":"55","author":"Gannedi","year":"2014","journal-title":"Tetrahedron Lett."},{"key":"2026090213094340400_cit29","doi-asserted-by":"publisher","first-page":"2275","DOI":"10.1021\/ja109932t","article-title":"Synthesis of docosasaccharide\narabinan motif of mycobacterial cell\nwall","volume":"133","author":"Ishiwata","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"2026090213094340400_cit30","doi-asserted-by":"publisher","first-page":"8411","DOI":"10.1021\/jo010570x","article-title":"Synthesis of di-to\nhexasaccharide 1, 2-linked \u03b2-mannopyranan\noligomers, a terminal S-linked tetrasaccharide congener and the corresponding\nBSA glycoconjugates","volume":"66","author":"Nitz","year":"2001","journal-title":"J. Org. Chem."},{"key":"2026090213094340400_cit31","doi-asserted-by":"publisher","first-page":"6728","DOI":"10.1021\/jo00101a035","article-title":"3, 4, 6-Tri-O-benzyl-.\nalpha.-D-arabino-hexopyranos-2-ulosyl\nBromide: A Versatile Glycosyl Donor for the Efficient Generation of.\nbeta.-D-Mannopyranosidic Linkages","volume":"59","author":"Lichtenthaler","year":"1994","journal-title":"J. Org. Chem."},{"key":"2026090213094340400_cit32","doi-asserted-by":"publisher","first-page":"6403","DOI":"10.1016\/j.bmc.2012.08.048","article-title":"Synthesis of Staphylococcus aureus type 5 capsular\npolysaccharide repeating unit using novel L-FucNAc and D-FucNAc synthons\nand immunochemical evaluation","volume":"20","author":"Danieli","year":"2012","journal-title":"Bioorg. Med.\nChem."},{"key":"2026090213094340400_cit33","doi-asserted-by":"publisher","first-page":"3417","DOI":"10.1016\/j.tetlet.2005.03.099","article-title":"4-Acetoxy-2,2-dimethylbutanoate:\na useful carbohydrate protecting group for the selective formation\nof \u03b2-(1\u21923)-d-glucans","volume":"46","author":"Yu","year":"2005","journal-title":"Tetrahedron\nLett."},{"key":"2026090213094340400_cit34","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1002\/chem.200701251","article-title":"A Double Regio-\nand Stereoselective Glycosylation Strategy for the Synthesis of N-Glycans","volume":"14","author":"Unverzagt","year":"2008","journal-title":"Chem. - Eur. J."},{"key":"2026090213094340400_cit35","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1002\/anie.200353105","article-title":"Reactivity-Based One-Pot Synthesis of Oligomannoses:\nDefining Antigens Recognized by 2G12, a Broadly Neutralizing Anti-HIV-1\nAntibody","volume":"43","author":"Lee","year":"2004","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026090213094340400_cit36","doi-asserted-by":"publisher","first-page":"4452","DOI":"10.1016\/j.tetlet.2012.06.061","article-title":"Facile construction\nof 1,2-cis glucosidic linkage using sequential oxidation\u2013reduction\nroute for synthesis of an ER processing \u03b1-glucosidase I substrate","volume":"53","author":"Iino","year":"2012","journal-title":"Tetrahedron Lett."},{"key":"2026090213094340400_cit37","doi-asserted-by":"publisher","first-page":"3072","DOI":"10.1021\/jo9800098","article-title":"Total Synthesis of Caloporoside","volume":"63","author":"Fu\u0308rstner","year":"1998","journal-title":"J. Org. 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Chem."},{"key":"2026090213094340400_cit42","doi-asserted-by":"publisher","first-page":"1706","DOI":"10.1039\/b407323a","article-title":"A convergent,\nversatile route to two synthetic conjugate anti-toxin malaria vaccines","author":"Seeberger","year":"2004","journal-title":"Chem. Commun."},{"key":"2026090213094340400_cit43","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1021\/jo0482864","article-title":"Synthesis of a 2,3,4-Triglycosylated\nRhamnoside Fragment of Rhamnogalacturonan-II Side Chain A Using a\nLate Stage Oxidation Approach","volume":"70","author":"Chauvin","year":"2005","journal-title":"J. Org. Chem."},{"key":"2026090213094340400_cit44","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1039\/p19870000009","article-title":"Synthesis of oligosaccharides corresponding\nto biological repeating units of Shigella flexneri variant Y polysaccharide. Part 1. Overall strategy, synthesis of\na key trisaccharide intermediate, and synthesis of a pentasaccharide","volume":"1","author":"Pinto","year":"1987","journal-title":"J. Chem. Soc., Perkin Trans. 1"},{"key":"2026090213094340400_cit45","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.1039\/c39810001240","article-title":"Benzyl trichloroacetimidate, a versatile reagent for\nacid-catalysed benzylation of hydroxy-groups","author":"Iversen","year":"1981","journal-title":"J. Chem. Soc., Chem. Commun."},{"key":"2026090213094340400_cit46","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1146\/annurev.iy.13.040195.003255","article-title":"T Cell-Independent Antigens Type\n2","volume":"13","author":"Mond","year":"1995","journal-title":"Annu. Rev. Immunol."},{"key":"2026090213094340400_cit47","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.4049\/jimmunol.157.6.2229","article-title":"A model for induction\nof T cell-independent humoral\nimmunity in response to polysaccharide antigens","volume":"157","author":"Snapper","year":"1996","journal-title":"J. Immunol."},{"key":"2026090213094340400_cit48","doi-asserted-by":"publisher","first-page":"2539","DOI":"10.1016\/j.carres.2003.07.008","article-title":"Immunology\nof bacterial polysaccharide antigens","volume":"338","author":"Weintraub","year":"2003","journal-title":"Carbohydr.\nRes."},{"key":"2026090213094340400_cit49","doi-asserted-by":"publisher","first-page":"S49","DOI":"10.1093\/infdis\/165-Supplement_1-S49","article-title":"Thymus-Independent\nand Thymus-Dependent Responses to Polysaccharide Antigens","volume":"165","author":"Stein","year":"1992","journal-title":"J. Infect. Dis."},{"key":"2026090213094340400_cit50","doi-asserted-by":"publisher","first-page":"2995","DOI":"10.1039\/c3sc50862e","article-title":"Synthetically\ndefined glycoprotein\nvaccines: current status and future directions","volume":"4","author":"Adamo","year":"2013","journal-title":"Chemical Science"},{"key":"2026090213094340400_cit51","doi-asserted-by":"publisher","first-page":"2615","DOI":"10.1016\/j.vaccine.2010.01.012","article-title":"Beta-glucan-CRM197 conjugates\nas candidates antifungal vaccines","volume":"28","author":"Bromuro","year":"2010","journal-title":"Vaccine"},{"key":"2026090213094340400_cit52","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2010.00141","article-title":"Evaluation of surrogate animal\nmodels of melioidosis","volume":"1","author":"Warawa","year":"2010","journal-title":"Front. Microbiol."},{"key":"2026090213094340400_cit53","doi-asserted-by":"publisher","first-page":"S111","DOI":"10.1016\/S0035-9203(08)70026-9","article-title":"Burkholderia pseudomallei: animal\nmodels of infection","volume":"102","author":"Titball","year":"2008","journal-title":"Trans. R. Soc. Trop. Med.\nHyg."},{"key":"2026090213094340400_cit54","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1128\/CVI.00113-13","article-title":"Protection\nagainst experimental melioidosis following immunisation with live Burkholderia thailandensis expressing manno-heptose capsule","volume":"20","author":"Scott","year":"2013","journal-title":"Clinical and Vaccine Immunology"},{"key":"2026090213094340400_cit55","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1006\/cimm.1994.1006","article-title":"Immune Responses to Bacterial Polysaccharides: Terminal\nEpitopes Are More Immunogenic Than Internal Structures","volume":"153","author":"Fernandez","year":"1994","journal-title":"Cell. 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Microbiol."},{"key":"2026090213094340400_cit58","doi-asserted-by":"publisher","first-page":"392170","DOI":"10.1155\/2014\/392170","article-title":"Protection\nagainst Experimental Melioidosis\nfollowing Immunisation with a Lipopolysaccharide-Protein Conjugate","volume":"2014","author":"Scott","year":"2014","journal-title":"J. Immunol. Res."},{"key":"2026090213094340400_cit59","doi-asserted-by":"publisher","first-page":"14240","DOI":"10.1073\/pnas.0403302101","article-title":"Genomic plasticity of\nthe causative agent of melioidosis","volume":"101","author":"Holden","year":"2004","journal-title":"Proc. Natl.\nAcad. Sci. U. S. 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