{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:50:53Z","timestamp":1791820253878,"version":"4.3.4"},"reference-count":71,"publisher":"American Chemical Society (ACS)","issue":"10","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35 GM146771"],"award-info":[{"award-number":["R35 GM146771"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Silk fibroin hydrogels are a promising platform for tissue engineering due to their biocompatibility and tunability, yet efficient cell recovery from gels remains a challenge. This work synthesizes reversible hydrogels by electrostatically-crosslinking carboxylated silk with linear and branched polyamine. Solvent choice (water, saline, or medium) and protein concentration impacted gel stiffness, mesh size, degradability, and function as an in vitro culture substrate. Hydrogels flowed as a liquid and recovered their viscoelastic properties when cycled under shear. Unlike enzymatically-crosslinked silk hydrogels, \u03b2-sheet content did not significantly increase during culture. Encapsulated mesenchymal stem cells proliferated, and live cells were recovered with high efficiency (&amp;gt;90%) by disrupting gels with shear. Lower protein concentrations resulted in hydrogels with lower stiffness, larger mesh size, and increased cell recovery. Electrostatic crosslinking of carboxylated fibroin is a viable route to silk hydrogels that permit encapsulation and subsequent retrieval of live stem cells for regenerative engineering applications.<\/jats:p>","DOI":"10.1021\/acs.biomac.5c02745","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:03:09Z","timestamp":1790784189000},"page":"6311-6327","source":"Crossref","is-referenced-by-count":0,"title":["Injectable Shear Thinning Hydrogels from Functionalized Silk Proteins for 3D Mammalian Cell Culture"],"prefix":"10.1021","volume":"27","author":[{"given":"Sanyukta A.","family":"Patil","sequence":"first","affiliation":[{"name":"University of Connecticut , , , ,","place":["Storrs, Connecticut, United States, 06269"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-0114","authenticated-orcid":true,"given":"Kelly A.","family":"Burke","sequence":"additional","affiliation":[{"name":"Polymer Science Program , , , ,","place":["Storrs, Connecticut, United States, 06269"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"issue":"18","key":"2026101210520774300_cit1","doi-asserted-by":"publisher","first-page":"6920","DOI":"10.1039\/d2ma00568a","article-title":"Self-Assembled Silk Fibroin Hydrogels: From Preparation to Biomedical Applications","volume":"3","author":"Onder","year":"2022","journal-title":"Mater. Adv."},{"issue":"1","key":"2026101210520774300_cit2","doi-asserted-by":"publisher","DOI":"10.1002\/smmd.20220011","article-title":"Silk Fibroin Hydrogels for Biomedical Applications","volume":"1","author":"Zhang","year":"2022","journal-title":"Smart Med."},{"key":"2026101210520774300_cit3","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.procbio.2022.12.012","article-title":"The Relationship of Rheological Properties and the Performance of Silk Fibroin Hydrogels in Tissue Engineering Application","volume":"125","author":"Elango","year":"2023","journal-title":"Process Biochemistry"},{"issue":"5","key":"2026101210520774300_cit4","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1039\/d0tb02099k","article-title":"Functional Silk Fibroin Hydrogels: Preparation, Properties and Applications","volume":"9","author":"Zheng","year":"2021","journal-title":"J. Mater. Chem. B"},{"key":"2026101210520774300_cit5","doi-asserted-by":"publisher","first-page":"118803","DOI":"10.1016\/j.ijpharm.2019.118803","article-title":"Hydrogel-Based Commercial Products for Biomedical Applications: A Review","volume":"573","author":"Cascone","year":"2020","journal-title":"Int. J. Pharm."},{"issue":"8","key":"2026101210520774300_cit6","doi-asserted-by":"publisher","first-page":"364","DOI":"10.3390\/polym9080364","article-title":"Hydrogel Based Sensors for Biomedical Applications: An Updated Review","volume":"9","author":"Tavakoli","year":"2017","journal-title":"Polymers (Basel)"},{"issue":"15","key":"2026101210520774300_cit7","doi-asserted-by":"publisher","first-page":"3023","DOI":"10.3390\/polym14153023","article-title":"Novel Trends in Hydrogel Development for Biomedical Applications: A Review","volume":"14","author":"Sa\u0301nchez-Cid","year":"2022","journal-title":"Polymers (Basel)"},{"key":"2026101210520774300_cit8","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.addr.2012.09.010","article-title":"Hydrogels for Biomedical Applications","volume":"64","author":"Hoffman","year":"2012","journal-title":"Adv. Drug Deliv. Rev."},{"key":"2026101210520774300_cit9","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202508278","article-title":"Cell-Delivering Injectable Hydrogels with Tunable Microporous Structures Improve Therapeutic Efficacy for Volumetric Muscle Loss","volume":"35","author":"Yasue","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"2026101210520774300_cit10","doi-asserted-by":"publisher","first-page":"153391","DOI":"10.1016\/j.cej.2024.153391","article-title":"Injectable Hydrogels for Bioelectronics: A Viable Alternative to Traditional Hydrogels","volume":"495","author":"Shu","year":"2024","journal-title":"Chem. Eng. J."},{"key":"2026101210520774300_cit11","doi-asserted-by":"publisher","first-page":"123374","DOI":"10.1016\/j.carbpol.2025.123374","article-title":"Injectable Self-Crosslinking Hyaluronic Acid\/Silk Fibroin Blend Hydrogel Based on Disulfide Bond","volume":"356","author":"Feng","year":"2025","journal-title":"Carbohydr. Polym."},{"issue":"12","key":"2026101210520774300_cit12","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.1016\/j.progpolymsci.2014.07.006","article-title":"In Situ-Forming Injectable Hydrogels for Regenerative Medicine","volume":"39","author":"Yang","year":"2014","journal-title":"Prog. Polym. Sci."},{"issue":"2","key":"2026101210520774300_cit13","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1021\/acs.chemrev.2c00179","article-title":"Self-Healing Injectable Hydrogels for Tissue Regeneration","volume":"123","author":"Bertsch","year":"2022","journal-title":"Chem. Rev."},{"key":"2026101210520774300_cit14","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.eurpolymj.2017.01.027","article-title":"A Review of the Designs and Prominent Biomedical Advances of Natural and Synthetic Hydrogel Formulations","volume":"88","author":"Gyles","year":"2017","journal-title":"Eur. Polym. J."},{"issue":"11","key":"2026101210520774300_cit15","doi-asserted-by":"publisher","first-page":"2702","DOI":"10.3390\/polym12112702","article-title":"Fundamental Concepts of Hydrogels: Synthesis, Properties, and Their Applications","volume":"12","author":"Bashir","year":"2020","journal-title":"Polymers (Basel)"},{"key":"2026101210520774300_cit16","doi-asserted-by":"publisher","first-page":"109842","DOI":"10.1016\/j.eurpolymj.2020.109842","article-title":"Tuning the Conformation and Mechanical Properties of Silk Fibroin Hydrogels","volume":"134","author":"Johari","year":"2020","journal-title":"Eur. Polym. J."},{"issue":"39","key":"2026101210520774300_cit17","doi-asserted-by":"publisher","first-page":"24085","DOI":"10.1039\/c7ra02682j","article-title":"A Silk Fibroin Hydrogel with Reversible Sol\u2212Gel Transition","volume":"7","author":"Yin","year":"2017","journal-title":"RSC Adv."},{"issue":"43","key":"2026101210520774300_cit18","doi-asserted-by":"publisher","first-page":"21630","DOI":"10.1021\/jp056350v","article-title":"Mechanisms of Silk Fibroin Sol\u2212 Gel Transitions","volume":"110","author":"Matsumoto","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"2026101210520774300_cit19","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.mattod.2023.03.027","article-title":"Emerging Silk Fibroin Materials and Their Applications: New Functionality Arising from Innovations in Silk Crosslinking","volume":"65","author":"Tran","year":"2023","journal-title":"Materials Today"},{"key":"2026101210520774300_cit20","doi-asserted-by":"publisher","first-page":"134834","DOI":"10.1016\/j.ijbiomac.2024.134834","article-title":"Bioactive Silk Fibroin Hydrogels: Unraveling the Potential for Biomedical Engineering","volume":"278","author":"Ferna\u0301ndez-Gonza\u0301lez","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"2","key":"2026101210520774300_cit21","doi-asserted-by":"publisher","first-page":"560","DOI":"10.7150\/thno.103491","article-title":"Silk Fibroin-Based Hydrogels for Cartilage Organoids in Osteoarthritis Treatment","volume":"15","author":"Shen","year":"2025","journal-title":"Theranostics"},{"issue":"7","key":"2026101210520774300_cit22","doi-asserted-by":"publisher","first-page":"2957","DOI":"10.1021\/acs.biomac.3c00098","article-title":"Versatile Potential of Photo-Cross-Linkable Silk Fibroin: Roadmap from Chemical Processing toward Regenerative Medicine and Biofabrication Applications","volume":"24","author":"Amirian","year":"2023","journal-title":"Biomacromolecules"},{"key":"2026101210520774300_cit23","doi-asserted-by":"publisher","first-page":"2967","DOI":"10.2147\/ijn.s106467","article-title":"Chemically Cross-Linked Silk Fibroin Hydrogel with Enhanced Elastic Properties, Biodegradability, and Biocompatibility","volume":"11","author":"Park","year":"2016","journal-title":"Int. J. Nanomedicine"},{"issue":"12","key":"2026101210520774300_cit24","doi-asserted-by":"publisher","first-page":"5484","DOI":"10.1038\/s41596-021-00622-1","article-title":"3D Bioprinted Silk Fibroin Hydrogels for Tissue Engineering","volume":"16","author":"Kim","year":"2021","journal-title":"Nat. Protoc."},{"key":"2026101210520774300_cit25","doi-asserted-by":"publisher","first-page":"119679","DOI":"10.1016\/j.biomaterials.2019.119679","article-title":"Digital Light Processing 3D Printed Silk Fibroin Hydrogel for Cartilage Tissue Engineering","volume":"232","author":"Hong","year":"2020","journal-title":"Biomaterials"},{"key":"2026101210520774300_cit26","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.polymer.2018.08.019","article-title":"Characterization of Silk Hydrogel Formed with Hydrolyzed Silk Fibroin-Methacrylate via Photopolymerization","volume":"153","author":"Kim","year":"2018","journal-title":"Polymer (Guildf)."},{"issue":"2","key":"2026101210520774300_cit27","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1021\/jf9031603","article-title":"Alternative Reaction Mechanism for the Cross-Linking of Gelatin with Glutaraldehyde","volume":"58","author":"Farris","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"2026101210520774300_cit28","doi-asserted-by":"publisher","first-page":"91","DOI":"10.3389\/fmats.2019.00091","article-title":"Fabrication and Characterization of Electrospun Silk Fibroin\/Gelatin Scaffolds Crosslinked with Glutaraldehyde Vapor","volume":"6","author":"Mohammadzadehmoghadam","year":"2019","journal-title":"Front. Mater."},{"issue":"10","key":"2026101210520774300_cit29","doi-asserted-by":"publisher","first-page":"2764","DOI":"10.1021\/bm800874q","article-title":"Novel Genipin-Cross-Linked Chitosan\/Silk Fibroin Sponges for Cartilage Engineering Strategies","volume":"9","author":"Silva","year":"2008","journal-title":"Biomacromolecules"},{"issue":"22","key":"2026101210520774300_cit30","doi-asserted-by":"publisher","first-page":"4419","DOI":"10.3390\/polym15224419","article-title":"Comparison of Silk Hydrogels Prepared via Different Methods","volume":"15","author":"Hua","year":"2023","journal-title":"Polymers (Basel)"},{"issue":"15","key":"2026101210520774300_cit31","doi-asserted-by":"publisher","first-page":"2912","DOI":"10.1039\/d1tb02502c","article-title":"Photocurable Silk Fibroin-Based Tissue Sealants with Enhanced Adhesive Properties for the Treatment of Corneal Perforations","volume":"10","author":"Tutar","year":"2022","journal-title":"J. Mater. Chem. B"},{"issue":"5","key":"2026101210520774300_cit32","doi-asserted-by":"publisher","first-page":"431","DOI":"10.3390\/gels9050431","article-title":"Application of Silk-Fibroin-Based Hydrogels in Tissue Engineering","volume":"9","author":"Lyu","year":"2023","journal-title":"Gels"},{"issue":"2","key":"2026101210520774300_cit33","doi-asserted-by":"publisher","first-page":"022004","DOI":"10.1088\/1748-605x\/abb615","article-title":"Crosslinking Strategies for Silk Fibroin Hydrogels: Promising Biomedical Materials","volume":"16","author":"Farokhi","year":"2021","journal-title":"Biomedical Materials"},{"issue":"8","key":"2026101210520774300_cit34","doi-asserted-by":"publisher","first-page":"1054","DOI":"10.1016\/j.biomaterials.2007.11.003","article-title":"Sonication-Induced Gelation of Silk Fibroin for Cell Encapsulation","volume":"29","author":"Wang","year":"2008","journal-title":"Biomaterials"},{"issue":"8","key":"2026101210520774300_cit35","doi-asserted-by":"publisher","first-page":"5069","DOI":"10.1021\/acs.biomac.5c00506","article-title":"Degumming Time Governs Self-Assembled Silk Fibroin Hydrogel Properties through Molecular Weight and Amino Acid Composition","volume":"26","author":"Pacheco","year":"2025","journal-title":"Biomacromolecules"},{"issue":"7","key":"2026101210520774300_cit36","doi-asserted-by":"publisher","first-page":"2044","DOI":"10.1016\/j.bpj.2009.07.028","article-title":"Vortex-Induced Injectable Silk Fibroin Hydrogels","volume":"97","author":"Yucel","year":"2009","journal-title":"Biophys. J."},{"issue":"6","key":"2026101210520774300_cit37","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1002\/adma.200902643","article-title":"Electrogelation for Protein Adhesives","volume":"22","author":"Leisk","year":"2010","journal-title":"Adv. Mater.:Compos. Carbon, Pap. Symp."},{"issue":"3","key":"2026101210520774300_cit38","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1021\/bm0345460","article-title":"Structure and Properties of Silk Hydrogels","volume":"5","author":"Kim","year":"2004","journal-title":"Biomacromolecules"},{"issue":"10","key":"2026101210520774300_cit39","doi-asserted-by":"publisher","first-page":"1704757","DOI":"10.1002\/adfm.201704757","article-title":"High-strength, Durable All-silk Fibroin Hydrogels with Versatile Processability toward Multifunctional Applications","volume":"28","author":"Zhu","year":"2018","journal-title":"Adv. Funct. Mater."},{"issue":"3","key":"2026101210520774300_cit40","doi-asserted-by":"publisher","first-page":"2200503","DOI":"10.1002\/mame.202200503","article-title":"An Injectable Enzymatically Crosslinked and Mechanically Tunable Silk Fibroin\/Chondroitin Sulfate Chondro-inductive Hydrogel","volume":"308","author":"Farokhi","year":"2023","journal-title":"Macromol. Mater. Eng."},{"key":"2026101210520774300_cit41","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.biomaterials.2017.03.046","article-title":"Enzymatically Crosslinked Silk-Hyaluronic Acid Hydrogels","volume":"131","author":"Raia","year":"2017","journal-title":"Biomaterials"},{"issue":"29","key":"2026101210520774300_cit42","doi-asserted-by":"publisher","first-page":"4615","DOI":"10.1002\/adfm.201400526","article-title":"Highly Tunable Elastomeric Silk Biomaterials","volume":"24","author":"Partlow","year":"2014","journal-title":"Adv. Funct. Mater."},{"issue":"17","key":"2026101210520774300_cit43","doi-asserted-by":"publisher","first-page":"1900644","DOI":"10.1002\/adhm.201900644","article-title":"Silk Hydrogels Crosslinked by the Fenton Reaction","volume":"8","author":"Choi","year":"2019","journal-title":"Adv. Healthc. Mater."},{"issue":"2","key":"2026101210520774300_cit44","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1021\/acs.biomac.0c01539","article-title":"Silk Hydrogels with Controllable Formation of Dityrosine, 3, 4-Dihydroxyphenylalanine, and 3, 4-Dihydroxyphenylalanine\u2212Fe3+ Complexes through Chitosan Particle-Assisted Fenton Reactions","volume":"22","author":"Choi","year":"2021","journal-title":"Biomacromolecules"},{"key":"2026101210520774300_cit45","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202508572","article-title":"Calcium Imparts Advanced Functionalities to Silk Hydrogels for Biofabrication and Biomedical Innovation","volume":"36","author":"Tran","year":"2025","journal-title":"Adv. Funct. Mater."},{"issue":"5","key":"2026101210520774300_cit46","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1021\/acs.bioconjchem.0c00168","article-title":"Enhancing the Carboxylation Efficiency of Silk Fibroin through the Disruption of Noncovalent Interactions","volume":"31","author":"Heichel","year":"2020","journal-title":"Bioconjug. Chem."},{"issue":"1","key":"2026101210520774300_cit47","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1002\/jbm.b.31875","article-title":"Effect of Processing on Silk-based Biomaterials: Reproducibility and Biocompatibility","volume":"99B","author":"Wray","year":"2011","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"issue":"6","key":"2026101210520774300_cit48","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1002\/mabi.201500013","article-title":"The Effect of Sterilization on Silk Fibroin Biomaterial Properties","volume":"15","author":"Rnjak-Kovacina","year":"2015","journal-title":"Macromol. Biosci."},{"issue":"1","key":"2026101210520774300_cit49","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.actbio.2013.09.004","article-title":"Single and Dual Crosslinked Oxidized Methacrylated Alginate\/PEG Hydrogels for Bioadhesive Applications","volume":"10","author":"Jeon","year":"2014","journal-title":"Acta Biomater."},{"issue":"12","key":"2026101210520774300_cit50","doi-asserted-by":"publisher","first-page":"5498","DOI":"10.1021\/acs.macromol.1c00361","article-title":"Aggregation and Rheology of a Triblock Supra-Amphiphilic Polymer Prepared by Ionic Self-Assembly of a Double-Hydrophilic Polyelectrolyte with an Oppositely Charged Surfactant in Aqueous Solution","volume":"54","author":"Chen","year":"2021","journal-title":"Macromolecules"},{"issue":"1","key":"2026101210520774300_cit51","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1039\/c1sm06281f","article-title":"The Influence of Charge Ratio on Transient Networks of Polyelectrolyte Complex Micelles","volume":"8","author":"Lemmers","year":"2012","journal-title":"Soft Matter"},{"issue":"18","key":"2026101210520774300_cit52","doi-asserted-by":"publisher","first-page":"6161","DOI":"10.1021\/ma0610109","article-title":"Determining Beta-Sheet Crystallinity in Fibrous Proteins by Thermal Analysis and Infrared Spectroscopy","volume":"39","author":"Hu","year":"2006","journal-title":"Macromolecules"},{"issue":"5","key":"2026101210520774300_cit53","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1002\/mabi.201500370","article-title":"Silk Fibroin Degradation Related to Rheological and Mechanical Properties","volume":"16","author":"Partlow","year":"2016","journal-title":"Macromol. Biosci."},{"issue":"44","key":"2026101210520774300_cit54","doi-asserted-by":"publisher","first-page":"14350","DOI":"10.1021\/ja046079f","article-title":"Dissolution and Regeneration of Bombyx Mori Silk Fibroin Using Ionic Liquids","volume":"126","author":"Phillips","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"2026101210520774300_cit55","doi-asserted-by":"publisher","first-page":"130282","DOI":"10.1016\/j.ijbiomac.2024.130282","article-title":"Reinforced Hydrogel Building via Formation of Alginate-Chitosan Double Network with PH & Salt-Responsiveness and Electric Conductivity for Soft Actuators","volume":"263","author":"Xu","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"3","key":"2026101210520774300_cit56","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1039\/d3cc04280d","article-title":"Injectable Cell-Laden Silk Acid Hydrogel","volume":"60","author":"Sun","year":"2024","journal-title":"Chemical Communications"},{"issue":"5","key":"2026101210520774300_cit57","doi-asserted-by":"publisher","first-page":"055037","DOI":"10.1088\/1748-605x\/ab99d4","article-title":"Silk Fibroin Reactive Inks for 3D Printing Crypt-like Structures","volume":"15","author":"Heichel","year":"2020","journal-title":"Biomedical Materials"},{"issue":"5","key":"2026101210520774300_cit58","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1002\/jbm.b.33088","article-title":"A Protocol for Rheological Characterization of Hydrogels for Tissue Engineering Strategies","volume":"102","author":"Zuidema","year":"2014","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"2026101210520774300_cit59","doi-asserted-by":"publisher","first-page":"119720","DOI":"10.1016\/j.biomaterials.2019.119720","article-title":"Enzymatically Crosslinked Silk and Silk-Gelatin Hydrogels with Tunable Gelation Kinetics, Mechanical Properties and Bioactivity for Cell Culture and Encapsulation","volume":"232","author":"Hasturk","year":"2020","journal-title":"Biomaterials"},{"issue":"4","key":"2026101210520774300_cit60","doi-asserted-by":"publisher","first-page":"288","DOI":"10.3390\/gels11040288","article-title":"Extremely Rapid Gelling Curcumin Silk-Tyrosine Crosslinked Hydrogels","volume":"11","author":"Sundarakrishnan","year":"2025","journal-title":"Gels"},{"issue":"6","key":"2026101210520774300_cit61","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1002\/bab.1552","article-title":"A Comparative Investigation of Bombyx Mori Silk Fibroin Hydrogels Generated by Chemical and Enzymatic Cross-linking","volume":"64","author":"Chirila","year":"2017","journal-title":"Biotechnol. Appl. Biochem."},{"key":"2026101210520774300_cit62","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.actbio.2014.09.013","article-title":"Impact of Silk Biomaterial Structure on Proteolysis","volume":"11","author":"Brown","year":"2015","journal-title":"Acta Biomater."},{"issue":"3","key":"2026101210520774300_cit63","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1007\/s10971-015-3662-z","article-title":"Structure and Properties of Protein-Based Fibrous Hydrogels Derived from Silk Fibroin and Sodium Alginate","volume":"74","author":"Ming","year":"2015","journal-title":"J. Solgel Sci. Technol."},{"key":"2026101210520774300_cit64","doi-asserted-by":"publisher","first-page":"109240","DOI":"10.1016\/j.polymdegradstab.2020.109240","article-title":"Beta-Sheet Content Significantly Correlates with the Biodegradation Time of Silk Fibroin Hydrogels Showing a Wide Range of Compressive Modulus","volume":"179","author":"Kambe","year":"2020","journal-title":"Polym. Degrad. Stab."},{"issue":"5","key":"2026101210520774300_cit65","doi-asserted-by":"publisher","first-page":"1678","DOI":"10.1021\/acs.biomac.0c00090","article-title":"Enzymatic Degradation of Bombyx Mori Silk Materials: A Review","volume":"21","author":"Guo","year":"2020","journal-title":"Biomacromolecules"},{"issue":"23","key":"2026101210520774300_cit66","doi-asserted-by":"publisher","first-page":"5912","DOI":"10.1073\/pnas.1621350114","article-title":"B12-Dependent Photoresponsive Protein Hydrogels for Controlled Stem Cell\/Protein Release","volume":"114","author":"Wang","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"34","key":"2026101210520774300_cit67","doi-asserted-by":"publisher","first-page":"4521","DOI":"10.1016\/j.biomaterials.2008.08.008","article-title":"Engineered Extracellular Matrices with Cleavable Crosslinkers for Cell Expansion and Easy Cell Recovery","volume":"29","author":"Zhang","year":"2008","journal-title":"Biomaterials"},{"issue":"46","key":"2026101210520774300_cit68","doi-asserted-by":"publisher","first-page":"11939","DOI":"10.1039\/d4tb01575d","article-title":"Disassembly of Self-Assembling Peptide Hydrogels as a Versatile Method for Cell Extraction and Manipulation","volume":"12","author":"Ligorio","year":"2024","journal-title":"J. Mater. Chem. B"},{"issue":"4","key":"2026101210520774300_cit69","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1021\/bm7012789","article-title":"Biodegradable Materials Based on Silk Fibroin and Keratin","volume":"9","author":"Vasconcelos","year":"2008","journal-title":"Biomacromolecules"},{"issue":"1","key":"2026101210520774300_cit70","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1021\/acs.biomac.5b01330","article-title":"Silk Fibroin Aqueous-Based Adhesives Inspired by Mussel Adhesive Proteins","volume":"17","author":"Burke","year":"2015","journal-title":"Biomacromolecules"},{"issue":"5","key":"2026101210520774300_cit71","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/j.ijbiomac.2010.03.007","article-title":"Different properties of electrospun fibrous scaffolds of separated heavy-chain and light-chain fibroins of Bombyx mori","volume":"46","author":"Wadbua","year":"2010","journal-title":"International Journal of Biological Macromolecules"}],"container-title":["Biomacromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biomac.5c02745","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article-pdf\/27\/10\/6311\/68714817\/bm5c02745.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article-pdf\/27\/10\/6311\/68714817\/bm5c02745.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:23:10Z","timestamp":1791818590000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article\/27\/10\/6311\/5442597\/Injectable-Shear-Thinning-Hydrogels-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":71,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,9,30]]},"published-print":{"date-parts":[[2026,10,12]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.biomac.5c02745","relation":{},"ISSN":["1525-7797","1526-4602"],"issn-type":[{"value":"1525-7797","type":"print"},{"value":"1526-4602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]}}}