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Over the past years, a variety of alkyne-based fluorogenic probes have been proposed to detect azides, paving the way for live cell imaging and biomaterial analysis. Unfortunately, undesirable photophysical properties and poor solubility in aqueous solutions hamper the widespread use of these fluorogenic compounds. This work describes a water-soluble azide probe with high fluorescence enhancement (130-fold increase in intensity) upon reaction. We find accurate determination of azide densities on a polymer scaffold at low micromolar concentrations in PBS buffer. Additionally, by functionalizing the probe with biomolecules, we built a single-step (bio)molecule labeling and analysis vehicle. As proof of principle, a peptide was conjugated to polymer scaffolds and conversions could be accurately followed in situ. Altogether, our probe is a promising new chemical biology tool for analysis in physiological conditions.<\/jats:p>","DOI":"10.1021\/acs.biomac.6c00253","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T12:12:26Z","timestamp":1784722346000},"page":"5139-5149","source":"Crossref","is-referenced-by-count":0,"title":["A Water-Soluble\nFluorogenic Probe to Quantify Azide\nConcentrations in Biologically Relevant Environments"],"prefix":"10.1021","volume":"27","author":[{"given":"Lotte","family":"Gerrits","sequence":"first","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]},{"name":"Institute for Chemical Immunology , Geert Grooteplein 28 , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Verdellen","sequence":"additional","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Peeters","sequence":"additional","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arwen","family":"Gelderman","sequence":"additional","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5949-2649","authenticated-orcid":true,"given":"Ulysse J.","family":"Gross","sequence":"additional","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behrad","family":"Shaghaghi","sequence":"additional","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]},{"name":"Radboudumc , , Geert Grooteplein 26 , ,","place":["Nijmegen, The Netherlands, 6525 GA"]},{"name":"Radboudumc , , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roel","family":"Hammink","sequence":"additional","affiliation":[{"name":"Radboudumc , , Geert Grooteplein 26 , ,","place":["Nijmegen, The Netherlands, 6525 GA"]},{"name":"Radboudumc , , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2760-191X","authenticated-orcid":true,"given":"Paul H. J.","family":"Kouwer","sequence":"additional","affiliation":[{"name":"Radboud University , , Heyendaalseweg 135 , ,","place":["Nijmegen, The Netherlands, 6525 AJ"]},{"name":"Institute for Chemical Immunology , Geert Grooteplein 28 , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"2026081008065701500_cit1","doi-asserted-by":"publisher","first-page":"5645","DOI":"10.1002\/anie.200601752","article-title":"Ruthenium-Induced Allylcarbamate\nCleavage in Living\nCells","volume":"45","author":"Streu","year":"2006","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026081008065701500_cit2","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1021\/acs.bioconjchem.5b00300","article-title":"Strain\nPromoted Click Chemistry of 2-or 8-Azidopurine and 5-Azidopyrimidine\nNucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application\nto Living Cell Fluorescent Imaging","volume":"26","author":"Zayas","year":"2015","journal-title":"Bioconj.\nChem."},{"key":"2026081008065701500_cit3","doi-asserted-by":"publisher","first-page":"11429","DOI":"10.1021\/jacs.6b01625","article-title":"A Bioorthogonal\nnear-Infrared Fluorogenic Probe for Mrna Detection","volume":"138","author":"Wu","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"2026081008065701500_cit4","doi-asserted-by":"publisher","first-page":"1490","DOI":"10.1039\/C6CC08805H","article-title":"A Cell-Permeable\nand Triazole-Forming Fluorescent Probe for Glycoconjugate Imaging\nin Live Cells","volume":"53","author":"Shie","year":"2017","journal-title":"Chem. 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