{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:48:36Z","timestamp":1791820116708,"version":"4.3.4"},"reference-count":47,"publisher":"American Chemical Society (ACS)","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JPMXP1122714694"],"award-info":[{"award-number":["JPMXP1122714694"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24H02272"],"award-info":[{"award-number":["24H02272"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K17785"],"award-info":[{"award-number":["24K17785"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Efficient gene delivery to mitochondria remains a major challenge in the development of organelle-targeted therapeutics. Here, we report the rational design and evaluation of helix stabilized mitochondria-targeting peptides (MTPs) via the incorporation of 2-aminoisobutyric acid (Aib), a noncanonical residue known to promote helical structure and increase membrane permeability. Three essential peptides were newly synthesized by systematically substituting alanine or glycine residues with Aib in a peptide derived from the mitochondrial outer membrane protein mitoNEET. Structural analyses using molecular dynamics (MD) simulations and circular dichroism (CD) spectroscopy confirmed increased helical content in the Aib-substituted peptides. Compared with the unmodified peptide, the Aib-substituted peptides exhibited significantly increased HeLa cell uptake and mitochondrial localization. Furthermore, conjugation of the designed MTPs with a cationic arginyl\u2212histidine repeat peptide enabled efficient delivery of plasmid DNA into mitochondria, leading to successful expression of mitochondrial-localized reporter genes. These findings demonstrate that Aib-induced helix stabilization is a powerful strategy for increasing mitochondrial gene delivery and provide a versatile platform for peptide-based mitochondrial therapeutics.<\/jats:p>","DOI":"10.1021\/acs.biomac.6c00322","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:02:18Z","timestamp":1790686938000},"page":"6339-6350","source":"Crossref","is-referenced-by-count":0,"title":["2-Aminoisobutylic Acid-Stabilized Helical Peptides for Selective Mitochondrial Gene Delivery"],"prefix":"10.1021","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1880-1167","authenticated-orcid":true,"given":"Kota","family":"Nomura","sequence":"first","affiliation":[{"name":"Kyoto University , , Kyoto Daigaku Katsura , , ,","place":["Nishikyo-ku, Kyoto, Japan, 615-8510"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayo","family":"Terada","sequence":"additional","affiliation":[{"name":"Kyoto University , , Kyoto Daigaku Katsura , , ,","place":["Nishikyo-ku, Kyoto, Japan, 615-8510"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Murakami","sequence":"additional","affiliation":[{"name":"Kyoto University , , Kyoto Daigaku Katsura , , ,","place":["Nishikyo-ku, Kyoto, Japan, 615-8510"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4342-2292","authenticated-orcid":true,"given":"Tomohiro","family":"Umeno","sequence":"additional","affiliation":[{"name":"Kyoto Prefectural University of Medicine , , 1-5 Shimogamo Hangicho , , ,","place":["Sakyo-ku, Kyoto, Japan, 606-0823"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Tateishi","sequence":"additional","affiliation":[{"name":"RIKEN Center for Sustainable Resource Science , , 2-1 Hirosawa , , ,","place":["Wako, Saitama, Japan, 351-0198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3691-3608","authenticated-orcid":true,"given":"Makoto","family":"Oba","sequence":"additional","affiliation":[{"name":"Kyoto Prefectural University of Medicine , , 1-5 Shimogamo Hangicho , , ,","place":["Sakyo-ku, Kyoto, Japan, 606-0823"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2199-7420","authenticated-orcid":true,"given":"Keiji","family":"Numata","sequence":"additional","affiliation":[{"name":"Kyoto University , , Kyoto Daigaku Katsura , , ,","place":["Nishikyo-ku, Kyoto, Japan, 615-8510"]},{"name":"RIKEN Center for Sustainable Resource Science , , 2-1 Hirosawa , , ,","place":["Wako, Saitama, Japan, 351-0198"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"4","key":"2026101210513174200_cit1","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1038\/s41580-020-0210-7","article-title":"The cell biology of mitochondrial membrane dynamics","volume":"21","author":"Giacomello","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"6","key":"2026101210513174200_cit2","doi-asserted-by":"publisher","first-page":"dmm048912","DOI":"10.1242\/dmm.048912","article-title":"Mitochondrial function in development and disease","volume":"14","author":"Rossmann","year":"2021","journal-title":"Dis. Models Mech."},{"issue":"7","key":"2026101210513174200_cit3","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1038\/s41556-018-0124-1","article-title":"The multifaceted contributions of mitochondria to cellular metabolism","volume":"20","author":"Spinelli","year":"2018","journal-title":"Nat. Cell Biol."},{"issue":"11","key":"2026101210513174200_cit4","doi-asserted-by":"publisher","first-page":"2601","DOI":"10.1016\/j.cell.2024.04.037","article-title":"Mitochondria at the crossroads of health and disease","volume":"187","author":"Suomalainen","year":"2024","journal-title":"Cell"},{"issue":"1","key":"2026101210513174200_cit5","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/s41392-024-02044-3","article-title":"Mitochondrial diseases: from molecular mechanisms to therapeutic advances","volume":"10","author":"Wen","year":"2025","journal-title":"Signal Transduction Targeted Ther."},{"issue":"1","key":"2026101210513174200_cit6","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1038\/s41392-024-01839-8","article-title":"Mitochondrial dysfunction: mechanisms and advances in therapy","volume":"9","author":"Zong","year":"2024","journal-title":"Signal Transduction Targeted Ther."},{"key":"2026101210513174200_cit7","doi-asserted-by":"publisher","first-page":"101922","DOI":"10.1016\/j.mito.2024.101922","article-title":"Beyond the membrane: Exploring non-viral methods for mitochondrial gene delivery","volume":"78","author":"Singh","year":"2024","journal-title":"Mitochondrion"},{"issue":"1","key":"2026101210513174200_cit8","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1186\/s12967-022-03685-0","article-title":"Current advances in gene therapy of mitochondrial diseases","volume":"20","author":"Soldatov","year":"2022","journal-title":"J. Transl. Med."},{"issue":"2","key":"2026101210513174200_cit9","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1021\/acsbiomaterials.1c01114","article-title":"Rational Designs at the Forefront of Mitochondria-Targeted Gene Delivery: Recent Progress and Future Perspectives","volume":"8","author":"Yoshinaga","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"1","key":"2026101210513174200_cit10","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/s41428-024-00973-y","article-title":"Peptide-mediated gene and protein delivery systems to plant mitochondria for modifying mitochondrial functions","volume":"57","author":"Abe","year":"2025","journal-title":"Polym. J."},{"issue":"9","key":"2026101210513174200_cit11","doi-asserted-by":"publisher","first-page":"1893","DOI":"10.1021\/acs.accounts.6b00277","article-title":"Peptide-Mediated Delivery of Chemical Probes and Therapeutics to Mitochondria","volume":"49","author":"Jean","year":"2016","journal-title":"Acc. Chem. Res."},{"issue":"3","key":"2026101210513174200_cit12","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1021\/acs.biochem.9b00857","article-title":"Mitochondrion-Targeting Peptides and Peptidomimetics: Recent Progress and Design Principles","volume":"59","author":"Kim","year":"2020","journal-title":"Biochemistry"},{"issue":"1","key":"2026101210513174200_cit13","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0962-8924(99)01684-0","article-title":"Protein translocation into mitochondria: the role of TIM complexes","volume":"10","author":"Bauer","year":"2000","journal-title":"Trends Cell Biol."},{"issue":"5","key":"2026101210513174200_cit14","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1038\/s41580-018-0092-0","article-title":"Mitochondrial proteins: from biogenesis to functional networks","volume":"20","author":"Pfanner","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"5","key":"2026101210513174200_cit15","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/S0092-8674(00)80691-1","article-title":"Structural Basis of Presequence Recognition by the Mitochondrial Protein Import Receptor Tom20","volume":"100","author":"Abe","year":"2000","journal-title":"Cell"},{"issue":"6","key":"2026101210513174200_cit16","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1002\/j.1460-2075.1986.tb04363.x","article-title":"A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers","volume":"5","author":"Roise","year":"1986","journal-title":"EMBO J."},{"issue":"5689","key":"2026101210513174200_cit17","doi-asserted-by":"publisher","first-page":"1466","DOI":"10.1126\/science.1099191","article-title":"Activation of Apoptosis in Vivo by a Hydrocarbon-Stapled BH3 Helix","volume":"305","author":"Walensky","year":"2004","journal-title":"Sci."},{"issue":"11","key":"2026101210513174200_cit18","doi-asserted-by":"publisher","first-page":"2039","DOI":"10.1042\/bcj20200290","article-title":"Rationally designed peptide-based inhibitor of A\u03b242 fibril formation and toxicity: a potential therapeutic strategy for Alzheimer\u2019s disease","volume":"477","author":"Horsley","year":"2020","journal-title":"Biochem. J."},{"issue":"6","key":"2026101210513174200_cit19","doi-asserted-by":"publisher","first-page":"6395","DOI":"10.1021\/acsomega.8b01030","article-title":"Extent of Helical Induction Caused by Introducing \u03b1-Aminoisobutyric Acid into an Oligovaline Sequence","volume":"3","author":"Tsuji","year":"2018","journal-title":"ACS Omega"},{"issue":"12","key":"2026101210513174200_cit20","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1002\/1439-7633(20021202)3:12&lt;1263::Aid-cbic1263&gt;3.0.Co;2-o","article-title":"A short Aib\/Ala-based peptide helix is as stable as an Ala-based peptide helix double its length","volume":"3","author":"Banerjee","year":"2002","journal-title":"ChemBioChem"},{"issue":"16","key":"2026101210513174200_cit21","doi-asserted-by":"publisher","first-page":"2815","DOI":"10.1002\/ejoc.201600327","article-title":"\u03b1-Helical Structures of Oligopeptides with an Alternating l-Leu-Aib Segment","volume":"2016","author":"Demizu","year":"2016","journal-title":"Eur. J. Org. Chem."},{"issue":"29","key":"2026101210513174200_cit22","doi-asserted-by":"publisher","first-page":"6747","DOI":"10.1021\/bi00481a001","article-title":"Structural characteristics of .alpha.-helical peptide molecules containing Aib residues","volume":"29","author":"Karle","year":"1990","journal-title":"Biochemistry"},{"issue":"11","key":"2026101210513174200_cit23","doi-asserted-by":"publisher","first-page":"5660","DOI":"10.1021\/acsbiomaterials.8b01451","article-title":"Plasmid DNA Delivery Using Cell-Penetrating Peptide Foldamers Composed of Arg\u2212Arg\u2212Aib Repeating Sequences","volume":"5","author":"Oba","year":"2019","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"8","key":"2026101210513174200_cit24","doi-asserted-by":"publisher","DOI":"10.1002\/psc.3479","article-title":"Total synthesis of the natural, medium-length, peptaibol pentadecaibinand study of the chemical features responsible for its membrane activity","volume":"29","author":"Morbiato","year":"2023","journal-title":"J. Pept. Sci."},{"issue":"6","key":"2026101210513174200_cit25","doi-asserted-by":"publisher","first-page":"3287","DOI":"10.1021\/acsbiomaterials.0c00182","article-title":"Artificial Cell-Penetrating Peptide Containing Periodic \u03b1-Aminoisobutyric Acid with Long-Term Internalization Efficiency in Human and Plant Cells","volume":"6","author":"Terada","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"19","key":"2026101210513174200_cit26","doi-asserted-by":"publisher","first-page":"5688","DOI":"10.1016\/j.bmcl.2011.08.030","article-title":"Cellular uptake of Aib-containing amphipathic helix peptide","volume":"21","author":"Wada","year":"2011","journal-title":"Bioorg. Med. Chem. Lett."},{"issue":"8","key":"2026101210513174200_cit27","doi-asserted-by":"publisher","first-page":"2306070","DOI":"10.1002\/adfm.202306070","article-title":"Design of an Artificial Peptide Inspired by Transmembrane Mitochondrial Protein for Escorting Exogenous DNA into the Mitochondria to Restore their Functions by Simultaneous Multiple Gene Expression","volume":"34","author":"Yoshinaga","year":"2024","journal-title":"Adv. Funct. Mater."},{"issue":"14","key":"2026101210513174200_cit28","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1021\/ja00897a025","article-title":"Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide","volume":"85","author":"Merrifield","year":"1963","journal-title":"J. Am. Chem. Soc."},{"key":"2026101210513174200_cit29","unstructured":"Case, D. A.; Betz, R. M.; Cerutti, D. S.; Cheatham III, T. E.; Darden, T. A.; Duke, R. E.; Giese, T. J.; Gohlke, H.; Goetz, A. W.; Homeyer, N.; Izadi, S.; Janowski, P.; Kaus, J.; Kovalenko, A.; Lee, T. S.; LeGrand, S.; Li, P.; Lin, C.; Luchko, T.; Luo, R.; Madej, B.; Mermelstein, D.; Merz, K. M.; Monard, G.; Nguyen, H.; Nguyen, H. T.; Omelyan, I.; Onufriev, A.; Roe, D. R.; Roitberg, A.; Sagui, C.; Simmerling, C. L.; Botello-Smith, W. M.; Swails, J.; Walker, R. C.; Wang, J.; Wolf, R. M.; Wu, X.; Xiao, L.; Kollman, P. A.\n          AMBER 2016; University of California: San Francisco: San Francisco, CA, 2016."},{"issue":"8","key":"2026101210513174200_cit30","doi-asserted-by":"publisher","first-page":"3696","DOI":"10.1021\/acs.jctc.5b00255","article-title":"ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB","volume":"11","author":"Maier","year":"2015","journal-title":"J. Chem. Theory Comput."},{"issue":"7","key":"2026101210513174200_cit31","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"issue":"6","key":"2026101210513174200_cit32","doi-asserted-by":"publisher","first-page":"3993","DOI":"10.1529\/biophysj.103.038422","article-title":"Automatic and quantitative measurement of protein-protein colocalization in live cells","volume":"86","author":"Costes","year":"2004","journal-title":"Biophys. J."},{"issue":"2","key":"2026101210513174200_cit33","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1039\/a809656b","article-title":"A water-soluble tetrazolium salt useful for colorimetric cell viability assay","volume":"36","author":"Tominaga","year":"1999","journal-title":"Anal. Commun."},{"issue":"6","key":"2026101210513174200_cit34","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1038\/s41589-019-0262-1","article-title":"Disruption of endocytosis through chemical inhibition of clathrin heavy chain function","volume":"15","author":"Dejonghe","year":"2019","journal-title":"Nat. Chem. Biol."},{"issue":"3","key":"2026101210513174200_cit35","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1083\/jcb.88.3.487","article-title":"Mechanism of action of cytochalasin: evidence that it binds to actin filament ends","volume":"88","author":"Brown","year":"1981","journal-title":"J. Cell Biol."},{"issue":"1","key":"2026101210513174200_cit36","doi-asserted-by":"publisher","first-page":"7268","DOI":"10.1038\/s41598-018-25677-1","article-title":"Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx moricypovirus","volume":"8","author":"Chen","year":"2018","journal-title":"Sci. Rep."},{"issue":"10","key":"2026101210513174200_cit37","doi-asserted-by":"publisher","first-page":"4438","DOI":"10.1073\/pnas.88.10.4438","article-title":"Isolation and expression of a cDNA encoding Renilla reniformis luciferase","volume":"88","author":"Lorenz","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"issue":"14","key":"2026101210513174200_cit38","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1021\/ja00897a025","article-title":"Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide","volume":"85","author":"Merrifield","year":"2002","journal-title":"J. Am. Chem. Soc."},{"issue":"22","key":"2026101210513174200_cit39","doi-asserted-by":"publisher","first-page":"4777","DOI":"10.1038\/sj.emboj.7601888","article-title":"Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states","volume":"26","author":"Saitoh","year":"2007","journal-title":"EMBO J."},{"issue":"4","key":"2026101210513174200_cit40","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.1021\/acsbiomaterials.0c01533","article-title":"Synthetic Mitochondria-Targeting Peptides Incorporating \u03b1-Aminoisobutyric Acid with a Stable Amphiphilic Helix Conformation in Plant Cells","volume":"7","author":"Terada","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"11","key":"2026101210513174200_cit41","doi-asserted-by":"publisher","first-page":"3547","DOI":"10.1021\/ACS.BIOMAC.6B01056","article-title":"Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights","volume":"17","author":"Chuah","year":"2016","journal-title":"Biomacromolecules"},{"issue":"21","key":"2026101210513174200_cit42","doi-asserted-by":"publisher","first-page":"7452","DOI":"10.1074\/jbc.RA119.012094","article-title":"The mitochondria-targeted peptide SS-31 binds lipid bilayers and modulates surface electrostatics as a key component of its mechanism of action","volume":"295","author":"Mitchell","year":"2020","journal-title":"J. Biol. Chem."},{"issue":"11","key":"2026101210513174200_cit43","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1021\/acscombsci.6b00081","article-title":"Searching for the Optimal Fluorophore to Label Antimicrobial Peptides","volume":"18","author":"Zhao","year":"2016","journal-title":"ACS Comb. Sci."},{"issue":"3","key":"2026101210513174200_cit44","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1083\/jcb.88.3.487","article-title":"Mechanism of action of cytochalasin: evidence that it binds to actin filament ends","volume":"88","author":"Brown","year":"1981","journal-title":"J. Cell Biol."},{"issue":"1","key":"2026101210513174200_cit45","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1002\/chem.201803174","article-title":"Bioconjugation with Maleimides: A Useful Tool for Chemical Biology","volume":"25","author":"Ravasco","year":"2019","journal-title":"Chem."},{"issue":"10","key":"2026101210513174200_cit46","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1134\/S0006297916100035","article-title":"DNA import into mitochondria","volume":"81","author":"Konstantinov","year":"2016","journal-title":"Biochemistry (Moscow)"},{"issue":"1","key":"2026101210513174200_cit47","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1093\/hmg\/ddi435","article-title":"Natural competence of mammalian mitochondria allows the molecular investigation of mitochondrial gene expression","volume":"15","author":"Koulintchenko","year":"2006","journal-title":"Hum. Mol. Genet."}],"container-title":["Biomacromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biomac.6c00322","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article-pdf\/27\/10\/6339\/68687116\/bm6c00322.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article-pdf\/27\/10\/6339\/68687116\/bm6c00322.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:09:13Z","timestamp":1791817753000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article\/27\/10\/6339\/5440585\/2-Aminoisobutylic-Acid-Stabilized-Helical-Peptides"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":47,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,9,29]]},"published-print":{"date-parts":[[2026,10,12]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.biomac.6c00322","relation":{},"ISSN":["1525-7797","1526-4602"],"issn-type":[{"value":"1525-7797","type":"print"},{"value":"1526-4602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]}}}