{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:33:40Z","timestamp":1786365220815,"version":"build-2736575974"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008375","name":"Universit?t Basel","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100008375","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011963","name":"Swiss Nanoscience Institute","doi-asserted-by":"publisher","award":["P2005"],"award-info":[{"award-number":["P2005"]}],"id":[{"id":"10.13039\/100011963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004219","name":"Paul Scherrer Institut","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100004219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Light-driven membrane proteins are attractive functional elements for biohybrid membranes, as they enable direct conversion of light energy into ion gradients. While microbial rhodopsins have been extensively studied in lipid bilayers, their controlled incorporation into synthetic polymer membranes remains challenging. Here, we investigate the reconstitution of the light-driven proton and sodium pump Krokinobacter eikastus rhodopsin 2 (KR2) into amphiphilic block copolymer membranes using a mild detergent-assisted strategy. KR2 and a C-terminal GFP fusion variant were incorporated into both solid-supported planar membranes and polymersomes under low concentrations of n-dodecyl-\u03b2-d-maltopyranoside. Membrane stability and protein incorporation were characterized using surface-sensitive and fluorescence-based techniques, while ion transport activity was assessed in polymersomes. The GFP fusion enabled quantitative assessment of membrane association and insertion behavior. Reconstituted KR2 variants retained light-driven ion transport activity in polymersomes. This straightforward approach supports the development of biohybrid systems with potential for light-driven ion transport, sensing, and energy-conversion applications.<\/jats:p>","DOI":"10.1021\/acs.biomac.6c00738","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T12:21:55Z","timestamp":1784204515000},"page":"5449-5460","source":"Crossref","is-referenced-by-count":0,"title":["Functional Insertion of the Light-Induced Ion Pump\nKR2 into Block Copolymer Membranes"],"prefix":"10.1021","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4871-1754","authenticated-orcid":true,"given":"Piotr","family":"Jasko","sequence":"first","affiliation":[{"name":"University of Basel , , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4002"]},{"name":"Paul Scherrer Institute , , ,","place":["Villigen, Switzerland, CH-5232"]},{"name":"Swiss Nanoscience Institute , Klingelbergstrasse 82 , ,","place":["Basel, Switzerland, CH-4056"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz S.","family":"Muthwill","sequence":"additional","affiliation":[{"name":"University of Basel , , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4002"]},{"name":"NCCR Molecular Systems Engineering , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryame","family":"Bina","sequence":"additional","affiliation":[{"name":"University of Basel , , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Frey","sequence":"additional","affiliation":[{"name":"Paul Scherrer Institute , , ,","place":["Villigen, Switzerland, CH-5232"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2933-7435","authenticated-orcid":true,"given":"Cora-Ann","family":"Schoenenberger","sequence":"additional","affiliation":[{"name":"University of Basel , , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Kammerer","sequence":"additional","affiliation":[{"name":"Paul Scherrer Institute , , ,","place":["Villigen, Switzerland, CH-5232"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7777-5355","authenticated-orcid":true,"given":"Cornelia G.","family":"Palivan","sequence":"additional","affiliation":[{"name":"University of Basel , , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4002"]},{"name":"Swiss Nanoscience Institute , Klingelbergstrasse 82 , ,","place":["Basel, Switzerland, CH-4056"]},{"name":"NCCR Molecular Systems Engineering , Mattenstrasse 22 , ,","place":["Basel, Switzerland, CH-4058"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"issue":"1","key":"2026081008075068400_cit1","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3390\/catal9010012","article-title":"Light-Driven\nBiocatalysis in Liposomes and Polymersomes:\nWhere Are We Now?","volume":"9","author":"Wang","year":"2019","journal-title":"Catalysts"},{"key":"2026081008075068400_cit2","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1146\/annurev-micro-031721-020452","article-title":"Microbial\nRhodopsins: The Last Two Decades","volume":"75","author":"Rozenberg","year":"2021","journal-title":"Annu. Rev.\nMicrobiol."},{"issue":"6","key":"2026081008075068400_cit3","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.1093\/oxfordjournals.jbchem.a131873","article-title":"Formations of Electrochemical\nProton Gradient and Adenosine Triphosphate in Proteoliposomes Containing\nPurified Adenosine Triphosphatase and Bacteriorhodopsin","volume":"82","author":"Sone","year":"1977","journal-title":"J. Biochem."},{"issue":"15","key":"2026081008075068400_cit4","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1002\/cbic.201900774","article-title":"Light-Driven ATP Regeneration\nin Diblock\/Grafted Hybrid Vesicles","volume":"21","author":"Kleineberg","year":"2020","journal-title":"ChemBioChem"},{"key":"2026081008075068400_cit5","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/j.jcis.2021.03.155","article-title":"Insertion and Activation\nof Functional Bacteriorhodopsin in a Floating Bilayer","volume":"597","author":"Mukhina","year":"2021","journal-title":"J. Colloid Interface Sci."},{"issue":"1","key":"2026081008075068400_cit6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s42004-018-0037-8","article-title":"Optimized Reconstitution of Membrane Proteins into Synthetic Membranes","volume":"1","author":"Goers","year":"2018","journal-title":"Commun. Chem."},{"issue":"2","key":"2026081008075068400_cit7","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1039\/C5CS00569H","article-title":"Bioinspired Polymer Vesicles and Membranes for Biological and Medical\nApplications","volume":"45","author":"Palivan","year":"2016","journal-title":"Chem. Soc. 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Natl. Acad. Sci. U. S. A."},{"issue":"14","key":"2026081008075068400_cit40","doi-asserted-by":"publisher","first-page":"6944","DOI":"10.1039\/D1NR01122G","article-title":"From Spherical\nCompartments to Polymer Films: Exploiting Vesicle Fusion to Generate\nSolid Supported Thin Polymer Membranes","volume":"13","author":"Kyropoulou","year":"2021","journal-title":"Nanoscale"},{"issue":"37","key":"2026081008075068400_cit41","doi-asserted-by":"publisher","first-page":"31224","DOI":"10.1021\/acsami.6b15199","article-title":"Influence\nof Solvent on the Structure of an Amphiphilic Block Copolymer in Solution\nand in Formation of an Integral Asymmetric Membrane","volume":"9","author":"Radjabian","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"10","key":"2026081008075068400_cit42","doi-asserted-by":"publisher","first-page":"525","DOI":"10.3390\/polym9100525","article-title":"Perpendicular Structure Formation\nof Block Copolymer Thin Films during\nThermal Solvent Vapor Annealing: Solvent and Thickness Effects","volume":"9","author":"Yang","year":"2017","journal-title":"Polymers"},{"issue":"23","key":"2026081008075068400_cit43","doi-asserted-by":"publisher","first-page":"8838","DOI":"10.1021\/ac201778h","article-title":"Hearing What You Cannot See and Visualizing\nWhat You Hear: Interpreting Quartz Crystal Microbalance Data from\nSolvated Interfaces","volume":"83","author":"Reviakine","year":"2011","journal-title":"Anal. 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Res."}],"container-title":["Biomacromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.biomac.6c00738","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article-pdf\/27\/8\/5449\/65978915\/acs.biomac.6c00738.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article-pdf\/27\/8\/5449\/65978915\/acs.biomac.6c00738.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:22:07Z","timestamp":1786364527000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/bomaf6\/article\/27\/8\/5449\/5212131\/Functional-Insertion-of-the-Light-Induced-Ion-Pump"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,7,16]]},"published-print":{"date-parts":[[2026,8,10]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.biomac.6c00738","relation":{},"ISSN":["1525-7797","1526-4602"],"issn-type":[{"value":"1525-7797","type":"print"},{"value":"1526-4602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]}}}