{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:33:31Z","timestamp":1786365211554,"version":"build-2736575974"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/na","name":"Austrian Research Promotion Agency","doi-asserted-by":"publisher","award":["COMET-CEST - no. 865864"],"award-info":[{"award-number":["COMET-CEST - no. 865864"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Italian Ministry for Foreign Affairs and International Cooperation","doi-asserted-by":"publisher","award":["Study in Italy"],"award-info":[{"award-number":["Study in Italy"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Next Generation EU - Italian NRRP","doi-asserted-by":"publisher","award":["Tech4You Ecosystem - no. ECS0000009"],"award-info":[{"award-number":["Tech4You Ecosystem - no. ECS0000009"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Collagen, the most abundant protein in mammals, plays a key role in tissue formation and mechanics due to its triple-helix structure. We used atomic force microscopy to study individual type-I and type-III human collagen molecules adsorbed on smooth mica surfaces from low-salt, near-neutral aqueous solutions. Statistical analysis of their two-dimensional contours revealed nonuniform curvature in both collagen types, which persisted after surface drying and molecular dehydration, owing to robust collagen\u2013mica adsorption. In addition, the angle between tangent vectors at the ends of molecular segments followed a non-Gaussian probability distribution, indicative of nonequilibrium quenching of fluctuations upon adsorption to mica. These results suggest that collagen either possesses an intrinsic three-dimensional curvature in solution or acquires a two-dimensional curvature upon adsorption. The first scenario has implications for the self-assembly and elasticity of collagen fibrils, whereas the second has implications in biomaterial design and tissue-engineering strategies.<\/jats:p>","DOI":"10.1021\/acs.biomac.6c00845","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:21:49Z","timestamp":1785849709000},"page":"5538-5545","source":"Crossref","is-referenced-by-count":0,"title":["Nonuniform Curvature\nand Bending Rigidity of Adsorbed\nCollagen Molecules in Aqueous Solution"],"prefix":"10.1021","volume":"27","author":[{"given":"Daniela A.","family":"Barraga\u0300n Rivera","sequence":"first","affiliation":[{"name":"Consiglio Nazionale Delle Ricerche - Istituto di Nanotecnologia (CNR-Nanotec) , via P. Bucci 33\/C , , ,","place":["Rende, Cosenza, Italy, 87036"]},{"name":"Universita\u0300 della Calabria , , via P. Bucci 31\/C , , ,","place":["Rende, Cosenza, Italy, 87036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria P.","family":"De Santo","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale Delle Ricerche - Istituto di Nanotecnologia (CNR-Nanotec) , via P. Bucci 33\/C , , ,","place":["Rende, Cosenza, Italy, 87036"]},{"name":"Universita\u0300 della Calabria , , via P. Bucci 31\/C , , ,","place":["Rende, Cosenza, Italy, 87036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Brunelli","sequence":"additional","affiliation":[{"name":"Universita\u0300 della Calabria , , via P. Bucci 4\/B , , ,","place":["Rende, Cosenza, Italy, 87036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierluigi","family":"Bilotto","sequence":"additional","affiliation":[{"name":"Technische Universita\u0308t Wien (TU Wien) - Institute of Engineering Design and Product Development , Getreidemarkt 9, BA , ,","place":["Vienna, Austria, 1060"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7588-9041","authenticated-orcid":true,"given":"Philipp J.","family":"Thurner","sequence":"additional","affiliation":[{"name":"Technische Universita\u0308t Wien (TU Wien) - Institute of Lightweight Design and Structural Biomechanics , Gumpendorfer Stra\u00dfe 7\/Objekt 8 , ,","place":["Vienna, Austria, A-1060"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7011-4036","authenticated-orcid":true,"given":"Guido","family":"Raos","sequence":"additional","affiliation":[{"name":"Politecnico di Milano , , via L. Mancinelli 7 , ,","place":["Milano, Italy, 20131"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3002-4022","authenticated-orcid":true,"given":"Bruno","family":"Zappone","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale Delle Ricerche - Istituto di Nanotecnologia (CNR-Nanotec) , via P. Bucci 33\/C , , ,","place":["Rende, Cosenza, Italy, 87036"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"2026081008083820500_cit1","doi-asserted-by":"publisher","first-page":"a004978","DOI":"10.1101\/cshperspect.a004978","article-title":"The collagen\nfamily","volume":"3","author":"Ricard-Blum","year":"2011","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"2026081008083820500_cit2","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1146\/annurev.biochem.77.032207.120833","article-title":"Collagen structure\nand stability","volume":"78","author":"Shoulders","year":"2009","journal-title":"Annu. Rev. Biochem."},{"key":"2026081008083820500_cit3","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.cbpa.2019.08.001","article-title":"Mechanics\nand structural stability\nof the collagen triple helix","volume":"53","author":"Kirkness","year":"2019","journal-title":"Curr. Opin. Chem.\nBiol."},{"key":"2026081008083820500_cit4","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1042\/BJ20151169","article-title":"Collagen structure:\nNew tricks from a very old dog","volume":"473","author":"Bella","year":"2016","journal-title":"Biochem. 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