{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:26:15Z","timestamp":1788380775359,"version":"build-2803163510"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"22","license":[{"start":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T00:00:00Z","timestamp":1604275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccbyncnd_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100003958","name":"Stichting voor de Technische Wetenschappen","doi-asserted-by":"publisher","award":["14979"],"award-info":[{"award-number":["14979"]}],"id":[{"id":"10.13039\/501100003958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sulfur reduction at hyperthermoacidophilic conditions represents a promising opportunity for metal sulfide precipitation from hot acidic metallurgical streams, avoiding costly cooling down. The suitability of mesophilic anaerobic sludges as the inoculum for sulfur-reducing bioreactors operated at high temperature and low pH was explored. We examined sludges from full-scale anaerobic reactors for sulfur-reducing activity at pH 2.0\u20133.5 and 70 or 80 \u00b0C, with H2 as an electron donor. At pH 3.5 in batch experiments, sulfidogenesis started within 4 days, reaching up to 100\u2013200 mg\u00b7L\u20131 of dissolved sulfide produced after 19\u201324 days, depending on the origin of the sludge. Sulfidogenesis resumed after removing H2S by flushing with nitrogen gas, indicating that sulfide was limiting the conversion. The best performing sludge was used to inoculate a 4 L gas-lift reactor fed with H2 as the electron donor, CO2 as the carbon source, and elemental sulfur as the electron acceptor. The reactor was operated in semibatch mode at a pH 3.5 and 80 \u00b0C, and stable sulfide production rates of 60\u201380 mg\u00b7L\u20131\u00b7d\u20131 were achieved for a period of 24 days, without formation of methane or acetate. Our results reveal the potential of mesophilic anaerobic sludges as seed material for sulfur-reducing bioprocesses operated at hyperthermoacidophilic conditions. The process needs further optimization of the volumetric sulfide production rate to gain relevance for practice.<\/jats:p>","DOI":"10.1021\/acs.est.0c02557","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T14:51:22Z","timestamp":1604328682000},"page":"14656-14663","source":"Crossref","is-referenced-by-count":14,"title":["Sulfur\nReduction at Hyperthermoacidophilic Conditions\nwith Mesophilic Anaerobic Sludge as the Inoculum"],"prefix":"10.1021","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-6678","authenticated-orcid":true,"given":"Adrian","family":"Hidalgo-Ulloa","sequence":"first","affiliation":[{"name":"Wageningen University & Research , , ,","place":["Wageningen, the Netherlands, 6708 WG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Sa\u0301nchez-Andrea","sequence":"additional","affiliation":[{"name":"Wageningen University & Research , , ,","place":["Wageningen, the Netherlands, 6708 WE"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cees","family":"Buisman","sequence":"additional","affiliation":[{"name":"Wageningen University & Research , , ,","place":["Wageningen, the Netherlands, 6708 WG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Weijma","sequence":"additional","affiliation":[{"name":"Wageningen University & Research , , ,","place":["Wageningen, the Netherlands, 6708 WG"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"2026090216065393700_cit1","doi-asserted-by":"publisher","first-page":"3863","DOI":"10.1016\/j.watres.2011.04.043","article-title":"Heavy metal removal\nin anaerobic semi-continuous stirred tank reactors by a consortium\nof sulfate-reducing bacteria","volume":"45","author":"Kieu","year":"2011","journal-title":"Water Res."},{"key":"2026090216065393700_cit2","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1111\/j.1751-7915.2011.00285.x","article-title":"Selective removal\nof transition metals from acidic\nmine waters by novel consortia of acidophilic sulfidogenic bacteria","volume":"5","author":"N\u030cancucheo","year":"2012","journal-title":"Microb. 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Doctoral\nthesis, Wageningen University, Wageningen, NL, 2007."},{"key":"2026090216065393700_cit10","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1038\/nrmicro1892","article-title":"The ecology and biotechnology of\nsulphate-reducing\nbacteria","volume":"6","author":"Muyzer","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"2026090216065393700_cit11","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.mineng.2017.08.008","article-title":"Environmentally sustainable\nacid mine drainage remediation: Research developments with a focus\non waste\/by-products","volume":"126","author":"Moodley","year":"2018","journal-title":"Miner. 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Microbiol."},{"key":"2026090216065393700_cit35","doi-asserted-by":"crossref","unstructured":"Fang, H.; Brown, B.; Young, D.; Nes\u030cic\u0301, S. In  Investigation of Elemental\nSulfur\nCorrosion Mechanisms, NACE Corrosion conference & Expo,\nHouston, Texas, USA 13-17 March 2011, 2011; Houston, Texas, USA 2011.","DOI":"10.5006\/C2011-11398"},{"key":"2026090216065393700_cit36","doi-asserted-by":"publisher","first-page":"95","DOI":"10.2343\/geochemj.34.95","article-title":"Isotopic\nfractionations accompanying sulfur hydrolysis","volume":"34","author":"Smith","year":"2000","journal-title":"Geochem. J."},{"key":"2026090216065393700_cit37","unstructured":"Rochelle, G. T.; Chang, H. T.; Chakravarti, S.\n          Research needs for acid\ngas kinetics and equilibria in alkanolamine systems. Final report\n          ,; Gas Research Institute: Chicago, 1991."},{"key":"2026090216065393700_cit38","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1007\/s11157-015-9387-1","article-title":"Methanogens, sulphate\nand heavy metals:\na complex system","volume":"14","author":"Paulo","year":"2015","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"2026090216065393700_cit39","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.tim.2007.02.005","article-title":"Life in acid: pH homeostasis\nin acidophiles","volume":"15","author":"Baker-Austin","year":"2007","journal-title":"Trends Microbiol."},{"key":"2026090216065393700_cit40","doi-asserted-by":"publisher","first-page":"75","DOI":"10.2166\/wst.2002.0294","article-title":"Competition for H2\nbetween sulfate reducers, methanogens\nand homoacetogens in a gas-lift reactor","volume":"45","author":"Weijma","year":"2002","journal-title":"Water\nSci. 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Microbiol."},{"key":"2026090216065393700_cit47","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1111\/1462-2920.14442","article-title":"Insight into the sulfur\nmetabolism of Desulfurella amilsii by differential proteomics","volume":"21","author":"Florentino","year":"2019","journal-title":"Environ. Microbiol."},{"key":"2026090216065393700_cit48","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1196\/annals.1419.016","article-title":"Old acetogens, new\nlight","volume":"1125","author":"Drake","year":"2008","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2026090216065393700_cit49","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1016\/j.resmic.2004.10.002","article-title":"Physiology of the thermophilic acetogen Moorella thermoacetica","volume":"155","author":"Drake","year":"2004","journal-title":"Res. 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