{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:19:42Z","timestamp":1789532382724,"version":"build-2803163510"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"1","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["101000733"],"award-info":[{"award-number":["101000733"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The application of synthetic phototrophic microbial consortia holds promise for sustainable bioenergy production. Nevertheless, strategies for the efficient construction and regulation of such consortia remain challenging. Applying tools of genetic engineering, this study successfully constructed a synthetic community of phototrophs using Rhodopseudomonas palustris (R. palustris) and an engineered strain of Synechocystis sp PCC6803 for acetate production (Synechocystis_acs), enabling the production of biohydrogen and fatty acids during nitrogen and carbon dioxide fixation. Elemental balance confirmed carbon capture and nitrogen fixation into the consortium. The strategy of circadian illumination effectively limited oxygen levels in the system, ensuring the activity of the nitrogenase in R. palustris, despite oxygenic photosynthesis happening in Synechocystis. When infrared light was introduced into the circadian illumination, the production of H2 (9.70 \u03bcmol mg\u20131) and fatty acids (especially C16 and C18) was significantly enhanced. Proteomic analysis indicated acetate exchange and light-dependent regulation of metabolic activities. Infrared illumination significantly stimulated the expression of proteins coding for nitrogen fixation, carbohydrate metabolism, and transporters in R. palustris, while constant white light led to the most upregulation of photosynthesis-related proteins in Synechocystis_acs. This study demonstrated the successful construction and light regulation of a phototrophic community, enabling H2 and fatty acid production through carbon and nitrogen fixation.<\/jats:p>","DOI":"10.1021\/acs.est.4c08629","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T23:32:07Z","timestamp":1734046327000},"page":"337-348","source":"Crossref","is-referenced-by-count":13,"title":["Engineering a Photoautotrophic\nMicrobial Coculture\ntoward Enhanced Biohydrogen Production"],"prefix":"10.1021","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5366-8387","authenticated-orcid":true,"given":"Minmin","family":"Pan","sequence":"first","affiliation":[{"name":"Helmholtz Centre for Environmental Research - UFZ , , ,","place":["Leipzig, Germany, 04318"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Amarante","family":"Colpo","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research - UFZ , , ,","place":["Leipzig, Germany, 04318"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stamatina","family":"Roussou","sequence":"additional","affiliation":[{"name":"Uppsala University , , Box 523 , ,","place":["Uppsala, Sweden, 75120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5550-4685","authenticated-orcid":true,"given":"Chang","family":"Ding","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research - UFZ , , ,","place":["Leipzig, Germany, 04318"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7256-0275","authenticated-orcid":true,"given":"Peter","family":"Lindblad","sequence":"additional","affiliation":[{"name":"Uppsala University , , Box 523 , ,","place":["Uppsala, Sweden, 75120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5006-0819","authenticated-orcid":true,"given":"Jens O.","family":"Kro\u0308mer","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research - UFZ , , ,","place":["Leipzig, Germany, 04318"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"issue":"12","key":"2026081212020841200_cit1","doi-asserted-by":"publisher","first-page":"2184","DOI":"10.1038\/s41564-019-0567-6","article-title":"Environmental stimuli drive a transition\nfrom cooperation to competition in synthetic phototrophic communities","volume":"4","author":"Zun\u0303iga","year":"2019","journal-title":"Nat. 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