{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:37:20Z","timestamp":1792679840564,"version":"4.5.0"},"reference-count":28,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2015,8,12]],"date-time":"2015-08-12T00:00:00Z","timestamp":1439337600000},"content-version":"vor","delay-in-days":86,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100006206","name":"Biological and Environmental Research","doi-asserted-by":"publisher","award":["DE-SC0006849"],"award-info":[{"award-number":["DE-SC0006849"]}],"id":[{"id":"10.13039\/100006206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mercury uptake in bacteria represents a key first step in the production and accumulation of methylmercury in biota. Previous experiments with mercury methylating bacteria have shown that Hg uptake is enhanced by some thiols, in particular cysteine, and that it is an energy-dependent process through heavy metal transporters [Schaefer et al. Environ. Sci. Technol.\u00a02014, 48, 3007]. In this study, we examine Hg uptake in the nonmethylating facultative aerobe, Shewanella oneidensis, under both anaerobic and aerobic conditions. Our results demonstrate similar characteristics of the Hg uptake system to those of the Hg methylating strains: (1) uptake is enhanced in the presence of some thiols but not others; (2) uptake is energy dependent as evidenced by inhibition by a protonophore, and (3) uptake is inhibited by high Zn(II) concentrations. Initial cellular uptake rates in S. oneidensis were remarkably similar under aerobic and fumarate-reducing conditions. These data support a similar Hg(II) uptake mechanism within the proteobacteria of accidental Hg(II) transport through heavy metal transporters with similar rates of uptake but differences in the ability to take up Hg bound to different thiols.<\/jats:p>","DOI":"10.1021\/acs.est.5b00676","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T12:34:58Z","timestamp":1431952498000},"page":"7432-7438","source":"Crossref","is-referenced-by-count":47,"title":["Effect\nof Thiols, Zinc, and Redox Conditions on Hg\nUptake in\n                    <i>Shewanella oneidensis<\/i>"],"prefix":"10.1021","volume":"49","author":[{"given":"Aleksandra","family":"Szczuka","sequence":"first","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois M. M.","family":"Morel","sequence":"additional","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffra K.","family":"Schaefer","sequence":"additional","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2015,5,18]]},"reference":[{"issue":"5","key":"2026090317123609000_cit1","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1021\/es405215v","article-title":"Effect\nof divalent metals on Hg(II)\nuptake and methylation by bacteria","volume":"48","author":"Schaefer","year":"2014","journal-title":"Environ.\nSci. Technol."},{"issue":"20","key":"2026090317123609000_cit2","doi-asserted-by":"crossref","first-page":"11810","DOI":"10.1021\/es403075t","article-title":"Mercury methylation by novel microorganisms from new environments","volume":"47","author":"Gilmour","year":"2013","journal-title":"Environ. Sci. Technol."},{"issue":"20","key":"2026090317123609000_cit3","doi-asserted-by":"crossref","first-page":"6325","DOI":"10.1128\/AEM.01556-13","article-title":"Mercury methylation\nby the methanogen Methanospirillum hungatei","volume":"79","author":"Yu","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"issue":"6125","key":"2026090317123609000_cit4","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1126\/science.1230667","article-title":"The genetic\nbasis for bacterial mercury\nmethylation","volume":"339","author":"Parks","year":"2013","journal-title":"Science"},{"issue":"4","key":"2026090317123609000_cit5","doi-asserted-by":"crossref","first-page":"967","DOI":"10.4319\/lo.2002.47.4.0967","article-title":"Evidence for facilitated\nuptake of Hg(II) by Vibrio anguillarum and Escherichia coli under anaerobic and aerobic conditions","volume":"47","author":"Golding","year":"2002","journal-title":"Limnol.\nOceanog."},{"issue":"2","key":"2026090317123609000_cit6","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/ngeo412","article-title":"High methylation rates of mercury bound to cysteine\nby Geobacter sulfurreducens","volume":"2","author":"Schaefer","year":"2009","journal-title":"Nat. Geosci."},{"issue":"21","key":"2026090317123609000_cit7","doi-asserted-by":"crossref","first-page":"8714","DOI":"10.1073\/pnas.1105781108","article-title":"Active transport, substrate specificity, and methylation\nof Hg(II) in anaerobic bacteria","volume":"108","author":"Schaefer","year":"2011","journal-title":"Proc. Natl.\nAcad. Sci."},{"issue":"2\u20133","key":"2026090317123609000_cit8","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0168-6445(03)00046-9","article-title":"Bacterial mercury resistance from\natoms to ecosystems","volume":"27","author":"Barkay","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"issue":"11","key":"2026090317123609000_cit9","doi-asserted-by":"crossref","first-page":"2904","DOI":"10.1111\/j.1462-2920.2010.02260.x","article-title":"A thermophilic bacterial\norigin and subsequent constraints by redox, light and salinity on\nthe evolution of the microbial mercuric reductase","volume":"12","author":"Barkay","year":"2010","journal-title":"Environ. Microbiol."},{"issue":"20","key":"2026090317123609000_cit10","doi-asserted-by":"crossref","first-page":"7138","DOI":"10.1128\/JB.187.20.7138-7145.2005","article-title":"Global transcriptome analysis of Shewanella oneidensis MR-1 exposed to different terminal electron acceptors","volume":"187","author":"Beliaev","year":"2005","journal-title":"J. Bacteriol."},{"issue":"2","key":"2026090317123609000_cit11","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/pmic.200500137","article-title":"Differential\nprotein expression in the metal-reducing bacterium Geobacter sulfurreducens strain PCA grown with fumarate or ferric citrate","volume":"6","author":"Khare","year":"2006","journal-title":"Proteomics"},{"issue":"12","key":"2026090317123609000_cit12","doi-asserted-by":"crossref","first-page":"7919","DOI":"10.1128\/AEM.01602-06","article-title":"Mercury methylation by dissimilatory iron-reducing\nbacteria","volume":"72","author":"Kerin","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"2026090317123609000_cit13","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1126\/science.240.4857.1319","article-title":"Bacterial manganese\nreduction and growth with manganese\noxide as the sole electron acceptor","volume":"240","author":"Myers","year":"1988","journal-title":"Science"},{"issue":"1\u20132","key":"2026090317123609000_cit14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0003-2670(00)80743-6","article-title":"Determination\nof volatile mercury species at the picogram\nlevel by low-temperature gas chromatography with cold-vapour atomic\nfluorescence detection","volume":"208","author":"Bloom","year":"1988","journal-title":"Anal. Chim. Acta"},{"issue":"5","key":"2026090317123609000_cit15","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1128\/aem.33.5.1225-1228.1977","article-title":"Use of Nucleopore filters for counting\nbacteria by fluorescence microscopy","volume":"33","author":"Hobbie","year":"1977","journal-title":"Appl. Environ.\nMicrobiol."},{"issue":"6","key":"2026090317123609000_cit16","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1021\/bi00894a001","article-title":"Metal chelates of some sulfur-containing amino acids","volume":"3","author":"Lenz","year":"1964","journal-title":"Biochemistry"},{"issue":"12","key":"2026090317123609000_cit17","doi-asserted-by":"crossref","first-page":"4741","DOI":"10.1021\/je200735r","article-title":"Sequestration\nof Hg2+ by some biologically important thiols","volume":"56","author":"Cardiano","year":"2011","journal-title":"J. Chem. Eng. Data"},{"issue":"20","key":"2026090317123609000_cit18","doi-asserted-by":"crossref","first-page":"7276","DOI":"10.1128\/AEM.00362-12","article-title":"Effect\nof inorganic and organic ligands on the bioavailability of methylmercury\nas determined by using a mer-lux bioreporter","volume":"78","author":"Ndu","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"issue":"12","key":"2026090317123609000_cit19","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1039\/C4MT00172A","article-title":"Hg(ii)\nbacterial biouptake: the role\nof anthropogenic and biogenic ligands present in solution and spectroscopic\nevidence of ligand exchange reactions at the cell surface","volume":"6","author":"Thomas","year":"2014","journal-title":"Metallomics"},{"issue":"19","key":"2026090317123609000_cit20","doi-asserted-by":"crossref","first-page":"6359","DOI":"10.1021\/ja00227a014","article-title":"Nuclear magnetic resonance studies\nof the solution chemistry of metal complexes. 25. Hg(thiol)3 complexes and Hg(II)-thiol ligand exchange kinetics","volume":"110","author":"Cheesman","year":"1988","journal-title":"J. Am. Chem. Soc."},{"issue":"12","key":"2026090317123609000_cit21","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.1128\/AEM.02993-10","article-title":"Sulfate-reducing\nbacterium Desulfovibrio desulfuricans ND132 as a\nmodel for understanding\nbacterial mercury methylation","volume":"77","author":"Gilmour","year":"2011","journal-title":"Appl. Environ.\nMicrobiol."},{"issue":"5","key":"2026090317123609000_cit22","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1023\/A:1024761324710","article-title":"Oxidation of thiol\ncompounds by molecular oxygen in aqueous solutions","volume":"52","author":"Bagiyan","year":"2003","journal-title":"Russ. Chem. Bull."},{"issue":"3","key":"2026090317123609000_cit23","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1128\/AEM.00717-07","article-title":"Effect of pH on\nIntracellular Accumulation\nof Trace Concentrations of Hg(II) in Escherichia coli under Anaerobic Conditions, as Measured Using a mer-lux Bioreporter","volume":"74","author":"Golding","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"issue":"6","key":"2026090317123609000_cit24","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1007\/s10534-013-9679-2","article-title":"The impact\nof ionic mercury on antioxidant\ndefenses in two mercury-sensitive anaerobic bacteria","volume":"26","author":"Wang","year":"2013","journal-title":"Biometals"},{"issue":"5","key":"2026090317123609000_cit25","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1021\/ez500107r","article-title":"Unexpected effects\nof gene deletion on interactions of mercury with the methylation-deficient\nmutant \u0394hgcAB","volume":"1","author":"Lin","year":"2014","journal-title":"Environ.\nSci. Technol. Lett."},{"issue":"20","key":"2026090317123609000_cit26","doi-asserted-by":"crossref","first-page":"6517","DOI":"10.1128\/AEM.01666-14","article-title":"Syntrophs dominate sequences associated\nwith the mercury-methylating gene hgcA in the Water\nConservation Areas of the Florida Everglades","volume":"80","author":"Bae","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"issue":"9","key":"2026090317123609000_cit27","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.1128\/AEM.04225-13","article-title":"Analysis\nof community\nstructure of Hg methylation gene (hgcA) in paddy\nsoils along an Hg gradient","volume":"80","author":"Liu","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"issue":"5","key":"2026090317123609000_cit28","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/1758-2229.12136","article-title":"Detection\nof a key Hg methylation gene, hgcA, in wetland soils","volume":"6","author":"Schaefer","year":"2014","journal-title":"Environ. Microbiol. Rep."}],"container-title":["Environmental Science &amp; Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.est.5b00676","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/esthag\/article-pdf\/49\/12\/7432\/67423391\/acs.est.5b00676.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/esthag\/article-pdf\/49\/12\/7432\/67423391\/acs.est.5b00676.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:12:47Z","timestamp":1788469967000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/esthag\/article\/49\/12\/7432\/1479391\/Effect-of-Thiols-Zinc-and-Redox-Conditions-on-Hg"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,18]]},"references-count":28,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2015,5,18]]},"published-print":{"date-parts":[[2015,6,16]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.est.5b00676","relation":{},"ISSN":["0013-936X","1520-5851"],"issn-type":[{"value":"0013-936X","type":"print"},{"value":"1520-5851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,18]]}}}