{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:18:22Z","timestamp":1788319102655,"version":"build-2803163510"},"reference-count":27,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T00:00:00Z","timestamp":1432252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R43AG046025"],"award-info":[{"award-number":["R43AG046025"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["HHSN261201300033C"],"award-info":[{"award-number":["HHSN261201300033C"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R43GM109682"],"award-info":[{"award-number":["R43GM109682"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R43AI106100"],"award-info":[{"award-number":["R43AI106100"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000064","name":"National Center for Complementary and Alternative Medicine","doi-asserted-by":"publisher","award":["R43AT008297"],"award-info":[{"award-number":["R43AT008297"]}],"id":[{"id":"10.13039\/100000064","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R43ES022360"],"award-info":[{"award-number":["R43ES022360"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R43ES023529"],"award-info":[{"award-number":["R43ES023529"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R44ES022360"],"award-info":[{"award-number":["R44ES022360"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Biomonitoring of pollutants and their metabolites and derivatives using biofluids provides new opportunities for spatiotemporal assessment of human risks to environmental exposures. Perfluorinated compounds (PFCs) have been used widely in industry and pose significant environmental concerns due to their stability and bioaccumulation in humans and animals. However, current methods for extraction and measurement of PFCs require relatively large volumes (over one hundred microliters) of blood samples, and therefore, are not suitable for frequent blood sampling and longitudinal biomonitoring of PFCs. We have developed a new microassay, enabled by our silicon microfluidic chip platform, for analyzing PFCs in small volumes (less than five microliters) of blood. Our assay integrates on-chip solid-phase extraction (SPE) with online nanoflow liquid chromatography-electrospray ionization-mass spectrometry (nanoLC-ESI-MS) detection. We demonstrated high sample recovery, excellent interday and intraday accuracy and precision, and a limit of detection down to 50 femtogram of PFCs, in one microliter of human plasma. We validated our assay performance using pooled human plasma and NIST SRM 1950 samples. Our microfluidic chip-based assay may enable frequent longitudinal biomonitoring of PFCs and other environmental toxins using a finger prick of blood, thereby providing new insights into their bioaccumulation, bioavailability, and toxicity.<\/jats:p>","DOI":"10.1021\/acs.est.5b01442","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T08:01:12Z","timestamp":1432281672000},"page":"6808-6814","source":"Crossref","is-referenced-by-count":6,"title":["Biomonitoring\nof Perfluorinated Compounds in a Drop\nof Blood"],"prefix":"10.1021","volume":"49","author":[{"given":"Pan","family":"Mao","sequence":"first","affiliation":[{"name":"Newomics Inc. , 5980 Horton Street , Suite 525, , ,","place":["Emeryville, California, United States, 94608"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daojing","family":"Wang","sequence":"additional","affiliation":[{"name":"Newomics Inc. , 5980 Horton Street , Suite 525, , ,","place":["Emeryville, California, United States, 94608"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2015,5,22]]},"reference":[{"issue":"8","key":"2026090123020521000_cit1","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1289\/ehp.1308015","article-title":"The blood\nexposome and its role in discovering causes of disease","volume":"122","author":"Rappaport","year":"2014","journal-title":"Environ. 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Cell Proteomics"},{"issue":"1","key":"2026090123020521000_cit4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.toxlet.2011.04.002","article-title":"Adductomics: Characterizing\nexposures to reactive electrophiles","volume":"213","author":"Rappaport","year":"2012","journal-title":"Toxicol.\nLett."},{"issue":"25","key":"2026090123020521000_cit5","doi-asserted-by":"crossref","first-page":"4041","DOI":"10.1016\/j.chroma.2009.11.061","article-title":"Solid-phase\nmicroextraction in bioanalysis: New devices and directions","volume":"1217","author":"Vuckovic","year":"2010","journal-title":"J. Chromatogr. A"},{"issue":"4","key":"2026090123020521000_cit6","doi-asserted-by":"crossref","first-page":"2784","DOI":"10.1021\/cr100203t","article-title":"Nondestructive\nsampling of living\nsystems using in vivo solid-phase microextraction","volume":"111","author":"Ouyang","year":"2011","journal-title":"Chem. Rev."},{"issue":"21","key":"2026090123020521000_cit7","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1021\/ac0106288","article-title":"Monolithic porous\npolymer for on-chip solid-phase extraction and preconcentration prepared\nby photoinitiated in situ polymerization within a microfluidic device","volume":"73","author":"Yu","year":"2001","journal-title":"Anal. Chem."},{"issue":"19","key":"2026090123020521000_cit8","doi-asserted-by":"crossref","first-page":"3622","DOI":"10.1002\/elps.200500121","article-title":"Coupling on-chip solid-phase extraction\nto electrospray mass spectrometry through an integrated electrospray\ntip","volume":"26","author":"Yang","year":"2005","journal-title":"Electrophoresis"},{"issue":"15","key":"2026090123020521000_cit9","doi-asserted-by":"crossref","first-page":"6082","DOI":"10.1021\/ac2011813","article-title":"Multinozzle emitter\narrays for nanoelectrospray mass spectrometry","volume":"83","author":"Mao","year":"2011","journal-title":"Anal. 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Proteome Res."},{"issue":"3","key":"2026090123020521000_cit13","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1021\/ac300003k","article-title":"Qualitative and quantitative analysis\nof tumor cell metabolism via stable isotope labeling assisted microfluidic\nchip electrospray ionization mass spectrometry","volume":"84","author":"Chen","year":"2012","journal-title":"Anal. Chem."},{"issue":"2","key":"2026090123020521000_cit14","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1021\/ac303164b","article-title":"Strategy for signaling\nmolecule detection by using an integrated microfluidic device coupled\nwith mass spectrometry to study cell-to-cell communication","volume":"85","author":"Mao","year":"2013","journal-title":"Anal. 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