{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:16:28Z","timestamp":1789488988870,"version":"build-2803163510"},"reference-count":92,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007689","name":"Universidade de Aveiro","doi-asserted-by":"publisher","award":["BI\/BIO\/9766\/2024"],"award-info":[{"award-number":["BI\/BIO\/9766\/2024"]}],"id":[{"id":"10.13039\/100007689","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/01190\/2018"],"award-info":[{"award-number":["CEECIND\/01190\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0094\/2020"],"award-info":[{"award-number":["LA\/P\/0094\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/50017\/2025"],"award-info":[{"award-number":["UID\/50017\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/05608\/2020"],"award-info":[{"award-number":["UIDB\/05608\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/05608\/2020"],"award-info":[{"award-number":["UIDP\/05608\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"H2020 Marie Sklodowska-Curie Actions","award":["956009"],"award-info":[{"award-number":["956009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sediments accumulate persistent and toxic compounds, yet assessing their effects on benthic species remains challenging. Especially antidepressants, such as fluoxetine, are of concern due to their sediment affinity and potential to disrupt neuroendocrine functions at low concentrations. This study investigated the chronic effects of fluoxetine in the sediment-dwelling worm Lumbriculus variegatus through two 28-day sediment exposures (0.00025\u201325 mg\/kg) conducted in accordance with OECD 225, with modifications. Endpoints encompassed multiple biological levels, including biochemical markers of neurotoxicity (cholinesterase), oxidative stress (catalase, glutathione-S-transferase, total glutathione), damage (lipid peroxidation), energy metabolism (lipids, sugars, proteins, electron transport system), physiological responses (oxygen consumption), three novel behavioral assays (chemical avoidance, feeding, phototaxis), and conventional apical endpoints (mortality, growth, reproduction). Effects on the non-conventional endpoints occurred at environmentally relevant sediment concentrations. Additionally, oxidative stress and behavioral responses were substantially more sensitive endpoints of fluoxetine exposure than the conventional endpoints growth, reproduction, and mortality. Integrating responses across biological levels, we propose a qualitative Adverse Outcome Pathway linking molecular and cellular events to individual-level effects. Our findings demonstrate that environmentally relevant fluoxetine concentrations elicit ecologically meaningful effects in L. variegatus that current regulatory frameworks would likely overlook, emphasizing the need for standardized behavioral assays in sediment risk assessment.<\/jats:p>","DOI":"10.1021\/acs.est.5c12822","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T20:34:28Z","timestamp":1788467668000},"page":"25192-25205","source":"Crossref","is-referenced-by-count":0,"title":["Digging into Understudied\nSediments: What\n                    <i>Lumbriculus variegatus<\/i>\n                    Reveals about Fluoxetine Exposure\nfrom the Cellular to the Individual Level"],"prefix":"10.1021","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9265-9328","authenticated-orcid":true,"given":"Jacqueline","family":"Hilgendorf","sequence":"first","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9872-6381","authenticated-orcid":true,"given":"Va\u0302nia","family":"Calisto","sequence":"additional","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3272-9360","authenticated-orcid":true,"given":"Diana","family":"Campos","sequence":"additional","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5754-0743","authenticated-orcid":true,"given":"Diogo N.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Fernandes","sequence":"additional","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5565-7454","authenticated-orcid":true,"given":"Diana L.D.","family":"Lima","sequence":"additional","affiliation":[{"name":"Polytechnic University of Coimbra , , Rua 5 de Outubro , ,","place":["Coimbra, Portugal, 3045-043"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper G.","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Aarhus University , , Ny Munkegade 114, Building 1540 , ,","place":["Aarhus C, Denmark, DK-8000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E\u0301rika M.L.","family":"Sousa","sequence":"additional","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5393-9623","authenticated-orcid":true,"given":"Susana","family":"Loureiro","sequence":"additional","affiliation":[{"name":"University of Aveiro , , Campus Universita\u0301rio de Santiago , ,","place":["Aveiro, Portugal, 3810-193"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"2026091511111610300_cit1","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.1002\/etc.5861","article-title":"Sediment Toxicity Tests: A Critical Review of Their\nUse in Environmental Regulations","volume":"43","author":"Leppanen","year":"2024","journal-title":"Environ. 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Pollut"},{"issue":"11","key":"2026091511111610300_cit20","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.1139\/F10-098","article-title":"Effects of Copper,\nCadmium, Lead, and Arsenic in a Live Diet on Juvenile Fish Growth","volume":"67","author":"Erickson","year":"2010","journal-title":"Can. J. Fish. Aquat. Sci"},{"issue":"4","key":"2026091511111610300_cit21","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.cbpc.2010.02.003","article-title":"Biogenic Amines Modulate\nPulse Rate in the Dorsal Blood Vessel of Lumbriculus Variegatus","volume":"151","author":"Crisp","year":"2010","journal-title":"Comp. Biochem. Physiol., Part C: toxicol. Pharmacol"},{"key":"2026091511111610300_cit22","doi-asserted-by":"publisher","first-page":"105015","DOI":"10.1016\/j.etap.2026.105015","article-title":"Quantifying Serotonin\nin Four Aquatic Invertebrates Using Enzyme-Linked Immunosorbent Assays\n(ELISA): Implications for Assessing Selective Serotonin Reuptake Inhibitor\n(SSRI) Effects","volume":"124","author":"Lautrette-Quinveros","year":"2026","journal-title":"Environ. Toxicol. Pharmacol"},{"key":"2026091511111610300_cit23","doi-asserted-by":"publisher","first-page":"780422","DOI":"10.3389\/fcell.2021.780422","article-title":"It Cuts Both Ways: An Annelid Model System for the\nStudy of Regeneration in the Laboratory and in the Classroom","volume":"9","author":"Martinez Acosta","year":"2021","journal-title":"Front. Cell Dev. 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Toxicol"},{"key":"2026091511111610300_cit42","volume-title":"ISO 17512: soil Quality -\nAvoidance Test for\nDetermining the Quality of Soilsand Effects of Chemicals on Behavior\n- Part 2: test With Collembolans (Folsomia Candida).","author":"ISO.","year":"2011"},{"key":"2026091511111610300_cit43","doi-asserted-by":"publisher","DOI":"10.2823\/4895485","volume-title":"Guidance\non the Application of the CLP Criteria\nPart 4: environmental Hazards and Part 5: additional Hazards","author":"ECHA.","year":"2024"},{"key":"2026091511111610300_cit44","doi-asserted-by":"publisher","first-page":"159486","DOI":"10.1016\/j.scitotenv.2022.159486","article-title":"Effects of Fluoxetine\non Fish: What Do We Know and Where Should We Focus Our Efforts in\nthe Future?","volume":"857","author":"Correia","year":"2023","journal-title":"Sci. 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