{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:16:15Z","timestamp":1792444575059,"version":"4.5.0"},"reference-count":96,"publisher":"American Chemical Society (ACS)","issue":"29","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42371064"],"award-info":[{"award-number":["42371064"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013494","name":"West Light Foundation, Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100013494","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Microbial CO2 fixation in alpine grassland soils is highly sensitive to warming and drought. Nitrogen inputs from grazing may stimulate autotrophic nitrifiers, including ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB). These nitrifiers assimilate carbon through CO2 fixation, but how their activity is associated with microbial CO2 fixation under warming and drought remains unclear. Here, we investigated microbial CO2 fixation and nitrification under altered temperature and moisture using urea-amended soil microcosms with 13CO2 labeling, DNA-stable isotope probing (DNA-SIP), metagenomics, and quantitative PCR. Warming increased CO2 fixation rates to 1.48\u20132.58 times those at 15 \u00b0C under moist conditions, whereas drought reduced it by 59\u201391%. Nitrification rates were positively correlated with CO2 fixation, whereas the CO2 fixation offset only 1.3\u201312.1% of associated N2O emissions (measured as CO2 equivalents). DNA-SIP and metagenomics indicated that nitrifiers contributed to microbial CO2 fixation, with AOA showing more pronounced 13C-labeling under combined warming and drought. Co-occurrence network indicated that AOA occupied more highly connected positions than AOB and NOB. This study provides microbial evidence that warming and drought reshape the linkage between nitrification and microbial CO2 fixation in urea-amended alpine grassland soil microcosms, with implications for carbon\u2013nitrogen cycling and greenhouse-gas feedbacks.<\/jats:p>","DOI":"10.1021\/acs.est.5c17815","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T18:51:18Z","timestamp":1784055078000},"page":"20304-20318","source":"Crossref","is-referenced-by-count":2,"title":["Nitrification Couples\nMicrobial CO2 Fixation\nto Warming and Drought Responses in Alpine Grassland Soils"],"prefix":"10.1021","volume":"60","author":[{"given":"Yirui","family":"Su","sequence":"first","affiliation":[{"name":"East China Normal University , , ,","place":["Shanghai, China, 200241"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianlian","family":"Fan","sequence":"additional","affiliation":[{"name":"Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences , ,","place":["Urumqi, China, 830011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengxing","family":"Chen","sequence":"additional","affiliation":[{"name":"East China Normal University , , ,","place":["Shanghai, China, 200241"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0199-7439","authenticated-orcid":true,"given":"Xiufeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Wang","sequence":"additional","affiliation":[{"name":"East China Normal University , , ,","place":["Shanghai, China, 200241"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-6548","authenticated-orcid":true,"given":"Uli","family":"Klu\u0308mper","sequence":"additional","affiliation":[{"name":"Technische Universita\u0308t Dresden , , ,","place":["Dresden, Germany, 01217"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1702-6322","authenticated-orcid":true,"given":"Guitao","family":"Shi","sequence":"additional","affiliation":[{"name":"East China Normal University , , ,","place":["Shanghai, China, 200241"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6025-1651","authenticated-orcid":true,"given":"Ping","family":"Han","sequence":"additional","affiliation":[{"name":"City University of Hong Kong , , , ,","place":["Kowloon, Hong Kong, China, 999077"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"2026072809091284700_cit1","unstructured":"Lee, H.; Romero, J.; IPCC..  Climate Change 2023: Synthesis Report: Contribution of Working Groups\nI, II and III to the Sixth Assessment Report of the Intergovernmental\nPanel on Climate ChangeIPCC: Geneva, Switzerland; 2023184."},{"issue":"3","key":"2026072809091284700_cit2","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1890\/12-0279.1","article-title":"Responses\nof Ecosystem Carbon Cycle to Experimental Warming: A Meta-Analysis","volume":"94","author":"Lu","year":"2013","journal-title":"Ecology"},{"key":"2026072809091284700_cit3","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.15050.1","article-title":"Recent Advances in Understanding\nGrasslands","volume":"7","author":"Stevens","year":"2018","journal-title":"F1000Research"},{"issue":"5826","key":"2026072809091284700_cit4","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1126\/science.1131634","article-title":"Global Desertification:\nBuilding\na Science for Dryland Development","volume":"316","author":"Reynolds","year":"2007","journal-title":"Science"},{"issue":"3","key":"2026072809091284700_cit5","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.ecolmodel.2007.02.024","article-title":"Desertification Due to Overgrazing\nin a Dynamic Commercial Livestock\u2013Grass\u2013Soil System","volume":"205","author":"Iba\u0301n\u0303ez","year":"2007","journal-title":"Ecol. Modell."},{"key":"2026072809091284700_cit6","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.agee.2011.11.009","article-title":"Carbon\nand Nitrogen in Degraded Brazilian Semi-Arid Soils Undergoing Desertification","volume":"148","author":"Sousa","year":"2012","journal-title":"Agric. Ecosyst. Environ."},{"issue":"10","key":"2026072809091284700_cit7","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1038\/s43017-021-00207-2","article-title":"Combatting Global Grassland\nDegradation","volume":"2","author":"Bardgett","year":"2021","journal-title":"Nat.\nRev. Earth Environ."},{"key":"2026072809091284700_cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.agrformet.2020.108085","article-title":"Growth Stage-Dependent\nResponses of Carbon Fixation Process of Alpine Grasslands to Climate\nChange over the Tibetan Plateau, China","volume":"291","author":"You","year":"2020","journal-title":"Agric.\nFor. Meteorol."},{"key":"2026072809091284700_cit9","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.5194\/hess-21-2249-2017","article-title":"Variation of Soil Hydraulic\nProperties with Alpine Grassland Degradation\nin the Eastern Tibetan Plateau","volume":"21","author":"Pan","year":"2017","journal-title":"Hydrol. Earth\nSyst. Sci."},{"key":"2026072809091284700_cit10","doi-asserted-by":"publisher","first-page":"3086","DOI":"10.1016\/j.jhydrol.2014.10.054","article-title":"Organic Matter Controls of Soil Water\nRetention in an Alpine Grassland and Its Significance for Hydrological\nProcesses","volume":"519","author":"Yang","year":"2014","journal-title":"J. Hydrol."},{"issue":"6","key":"2026072809091284700_cit11","doi-asserted-by":"publisher","DOI":"10.3390\/su12062523","article-title":"Light Grazing\nSignificantly Reduces Soil Water Storage in Alpine\nGrasslands on the Qinghai-Tibet Plateau","volume":"12","author":"Guo","year":"2020","journal-title":"Sustainability"},{"issue":"6479","key":"2026072809091284700_cit12","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1126\/science.aay5958","article-title":"Global Ecosystem Thresholds\nDriven by Aridity","volume":"367","author":"Berdugo","year":"2020","journal-title":"Science"},{"issue":"1","key":"2026072809091284700_cit13","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5799","article-title":"Aridity Threshold in\nControlling Ecosystem Nitrogen\nCycling in Arid and Semi-Arid Grasslands","volume":"5","author":"Wang","year":"2014","journal-title":"Nat.\nCommun."},{"issue":"1","key":"2026072809091284700_cit14","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04934-9","article-title":"Large Perturbations in CO2 Flux and Subsequent Chemosynthesis Are Induced in Agricultural Soil\nby the Addition of Elemental Sulfur","volume":"7","author":"Kelleher","year":"2017","journal-title":"Sci. Rep."},{"issue":"8","key":"2026072809091284700_cit15","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1038\/s41561-019-0387-6","article-title":"Direct\nObservation of Permafrost Degradation and Rapid Soil Carbon Loss in\nTundra","volume":"12","author":"Plaza","year":"2019","journal-title":"Nat. Geosci."},{"issue":"5","key":"2026072809091284700_cit16","doi-asserted-by":"publisher","first-page":"1839","DOI":"10.1111\/gcb.14603","article-title":"Effects of Climate\nWarming on Carbon\nFluxes in Grasslands\u2500 A Global Meta-Analysis","volume":"25","author":"Wang","year":"2019","journal-title":"Global Change Biol."},{"key":"2026072809091284700_cit17","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.soilbio.2018.09.034","article-title":"Desert and Steppe Soils Exhibit Lower\nAutotrophic Microbial Abundance but Higher Atmospheric CO2 Fixation Capacity than Meadow Soils","volume":"127","author":"Zhao","year":"2018","journal-title":"Soil\nBiol. Biochem."},{"key":"2026072809091284700_cit18","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2020.107950","article-title":"Rates of Dark\nCO2 Fixation Are Driven by Microbial Biomass in a Temperate\nForest Soil","volume":"150","author":"Akinyede","year":"2020","journal-title":"Soil Biol. Biochem."},{"issue":"2","key":"2026072809091284700_cit19","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1038\/s41564-020-00811-w","article-title":"Trace Gas Oxidizers\nAre Widespread and Active Members of Soil Microbial Communities","volume":"6","author":"Bay","year":"2021","journal-title":"Nat. Microbiol."},{"issue":"7","key":"2026072809091284700_cit20","doi-asserted-by":"publisher","first-page":"2328","DOI":"10.1128\/AEM.06881-11","article-title":"Significant Role for\nMicrobial Autotrophy in the Sequestration of Soil Carbon","volume":"78","author":"Yuan","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"issue":"5490","key":"2026072809091284700_cit21","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1126\/science.290.5490.291","article-title":"The Global\nCarbon Cycle: A Test of Our Knowledge of\nEarth as a System","volume":"290","author":"Falkowski","year":"2000","journal-title":"Science"},{"key":"2026072809091284700_cit22","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.143282","article-title":"Soil Microbial CO2 Fixation Plays a Significant Role in\nTerrestrial Carbon Sink\nin a Dryland Ecosystem: A Four-Year Small-Scale Field-Plot Observation\non the Tibetan Plateau","volume":"761","author":"Chen","year":"2021","journal-title":"Sci. Total Environ."},{"key":"2026072809091284700_cit23","doi-asserted-by":"publisher","DOI":"10.3389\/fenvs.2023.1113538","article-title":"Grazing Disturbance Significantly\nDecreased Soil Organic Carbon Contents\nof Alpine Grasslands on the Tibetan Plateau","volume":"11","author":"Yang","year":"2023","journal-title":"Front. Environ. Sci."},{"key":"2026072809091284700_cit24","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.973662","article-title":"Grazing during the Grassland Greenup\nPeriod Promotes\nPlant Species Richness in Alpine Grassland in Winter Pastures","volume":"13","author":"Wei","year":"2022","journal-title":"Front. Plant Sci."},{"issue":"6","key":"2026072809091284700_cit25","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1080\/10409230390267446","article-title":"Metabolism of Inorganic\nN Compounds by Ammonia-Oxidizing\nBacteria","volume":"38","author":"Arp","year":"2003","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"issue":"7058","key":"2026072809091284700_cit26","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1038\/nature03911","article-title":"Isolation of an Autotrophic Ammonia-Oxidizing Marine\nArchaeon","volume":"437","author":"Ko\u0308nneke","year":"2005","journal-title":"Nature"},{"issue":"9","key":"2026072809091284700_cit27","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/j.tim.2016.05.004","article-title":"A New Perspective on Microbes Formerly\nKnown as Nitrite-Oxidizing Bacteria","volume":"24","author":"Daims","year":"2016","journal-title":"Trends\nMicrobiol."},{"issue":"4","key":"2026072809091284700_cit28","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/s42832-020-0052-4","article-title":"Soil Microbial\nCarbon Pump: Mechanism and Appraisal","volume":"2","author":"Liang","year":"2020","journal-title":"Soil Ecol.\nLett."},{"issue":"10","key":"2026072809091284700_cit29","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiy163","article-title":"Autotrophic Carbon Fixation Strategies\nUsed by Nitrifying\nProkaryotes in Freshwater Lakes","volume":"94","author":"Alfreider","year":"2018","journal-title":"FEMS Microbiol.\nEcol."},{"issue":"5","key":"2026072809091284700_cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104333","article-title":"Niche Differentiation of Ammonia-Oxidizing\nArchaea and Related Autotrophic Carbon Fixation Potential in the Water\nColumn of the South China Sea","volume":"25","author":"Wu","year":"2022","journal-title":"iScience"},{"issue":"11","key":"2026072809091284700_cit31","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1016\/j.dsr2.2008.07.024","article-title":"A First Insight into the Occurrence\nand Expression of Functional AmoA and AccA Genes of Autotrophic and Ammonia-Oxidizing Bathypelagic Crenarchaeota of Tyrrhenian Sea","volume":"56","author":"Yakimov","year":"2009","journal-title":"Deep\nSea Res., Part II"},{"issue":"7","key":"2026072809091284700_cit32","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1093\/jxb\/erm297","article-title":"Multiple Rubisco\nForms in Proteobacteria: Their Functional\nSignificance in Relation to CO2 Acquisition by the CBB\nCycle","volume":"59","author":"Badger","year":"2008","journal-title":"J. Exp. Bot."},{"issue":"7","key":"2026072809091284700_cit33","doi-asserted-by":"publisher","first-page":"2754","DOI":"10.1111\/1462-2920.13786","article-title":"CO2 Assimilation\nStrategies in Stratified\nLakes: Diversity and Distribution Patterns of Chemolithoautotrophs","volume":"19","author":"Alfreider","year":"2017","journal-title":"Environ. Microbiol."},{"issue":"4","key":"2026072809091284700_cit34","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1007\/s42832-020-0044-4","article-title":"Compositional\nVariations of Active Autotrophic Bacteria in Paddy Soils with Elevated\nCO2 and Temperature","volume":"2","author":"Zhu","year":"2020","journal-title":"Soil Ecol. Lett."},{"key":"2026072809091284700_cit35","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01373","article-title":"Nitrification Is a Primary Driver of Nitrous Oxide Production in\nLaboratory Microcosms from Different Land-Use Soils","volume":"7","author":"Liu","year":"2016","journal-title":"Front. Microbiol."},{"issue":"1621","key":"2026072809091284700_cit36","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0122","article-title":"Nitrous Oxide\nEmissions from Soils: How Well Do We Understand the Processes and\nTheir Controls?","volume":"368","author":"Butterbach-Bahl","year":"2013","journal-title":"Philos. Trans. R. Soc., B"},{"key":"2026072809091284700_cit37","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2021.108528","article-title":"Offsetting N2O Emissions through Nitrifying CO2 Fixation in Grassland\nSoil","volume":"165","author":"Xia","year":"2022","journal-title":"Soil Biol. Biochem."},{"issue":"2","key":"2026072809091284700_cit38","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1111\/j.1462-2920.2008.01783.x","article-title":"Dynamics and Functional Relevance\nof Ammonia-oxidizing Archaea in Two Agricultural Soils","volume":"11","author":"Schauss","year":"2009","journal-title":"Environ. Microbiol."},{"issue":"12","key":"2026072809091284700_cit39","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1111\/j.1461-0248.2008.01251.x","article-title":"Thermal\nAdaptation of Soil Microbial Respiration to Elevated Temperature","volume":"11","author":"Bradford","year":"2008","journal-title":"Ecol. Lett."},{"key":"2026072809091284700_cit40","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejsobi.2020.103267","article-title":"Responses of Soil Ammonia-Oxidizing\nBacteria and Archaea to Short-Term Warming and Nitrogen Input in a\nSemi-Arid Grassland on the Loess Plateau","volume":"102","author":"Wang","year":"2021","journal-title":"Eur.\nJ. Soil Biol."},{"issue":"1","key":"2026072809091284700_cit41","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1146\/annurev-ecolsys-110617-062614","article-title":"Life in\nDry Soils: Life in Dry Soils: Effects of Drought on Soil Microbial\nCommunities and Processes","volume":"49","author":"Schimel","year":"2018","journal-title":"Annu. Rev. Ecol.\nEvol. Syst."},{"issue":"21","key":"2026072809091284700_cit42","doi-asserted-by":"publisher","first-page":"6003","DOI":"10.5194\/bg-11-6003-2014","article-title":"Effects of Drought on Nitrogen Turnover\nand Abundances of Ammonia-Oxidizers in Mountain Grassland","volume":"11","author":"Fuchslueger","year":"2014","journal-title":"Biogeosciences"},{"key":"2026072809091284700_cit43","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00219","article-title":"Deciphering Microbial\nInteractions and Detecting Keystone\nSpecies with Co-Occurrence Networks","volume":"5","author":"Berry","year":"2014","journal-title":"Front.\nMicrobiol."},{"key":"2026072809091284700_cit44","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-54537-y","article-title":"Soil Fungi Remain Active\nand Invest in Storage Compounds during Drought Independent of Future\nClimate Conditions","volume":"15","author":"Canarini","year":"2024","journal-title":"Nat. Commun."},{"issue":"3","key":"2026072809091284700_cit45","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1111\/gcb.16448","article-title":"Low Soil Moisture Suppresses the Thermal Compensatory Response of\nMicrobial Respiration","volume":"29","author":"Li","year":"2023","journal-title":"Global Change Biol."},{"key":"2026072809091284700_cit46","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1007\/s00374-018-1276-7","article-title":"Soil N Transformation\nMechanisms Can Effectively Conserve N in Soil under Saturated Conditions\nCompared to Unsaturated Conditions in Subtropical China","volume":"54","author":"Zhang","year":"2018","journal-title":"Biol. Fertil. Soils"},{"issue":"3","key":"2026072809091284700_cit47","doi-asserted-by":"publisher","first-page":"1926","DOI":"10.1111\/gcb.14937","article-title":"Dark Microbial\nCO2 Fixation in Temperate Forest Soils Increases with CO2 Concentration","volume":"26","author":"Spohn","year":"2020","journal-title":"Global Change Biol."},{"issue":"1","key":"2026072809091284700_cit48","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1017\/S0021859600021572","article-title":"Determination\nof Nitrogen in Soil by the Kjeldahl Method","volume":"55","author":"Bremner","year":"1960","journal-title":"J. Agric. Sci."},{"key":"2026072809091284700_cit49","unstructured":"Masson-Delmotte, V.; Zhai, P.; Pirani, A.; Connors, S. L.; Pe\u0301an, C.; Berger, S.; Caud, N.; Chen, Y.; Goldfarb, L.; Gomis, M. I.; IPCC.; et al.; .  Summary\nfor Policymakers Climate Change 2021: The Physical Science Basis.\nContribution of Working Group I to the Sixth Assessment Report of\nthe Intergovernmental Panel on Climate ChangeCambridge University Press: Cambridge,\nUnited Kingdom and New York, NY, USA; 2021."},{"issue":"1","key":"2026072809091284700_cit50","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-38","article-title":"Removing\nNoise From Pyrosequenced Amplicons","volume":"12","author":"Quince","year":"2011","journal-title":"BMC Bioinf."},{"issue":"1","key":"2026072809091284700_cit51","doi-asserted-by":"publisher","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt Removes\nAdapter Sequences from High-Throughput Sequencing Reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"issue":"19","key":"2026072809091284700_cit52","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search\nand Clustering Orders of Magnitude Faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"issue":"17","key":"2026072809091284700_cit53","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An Ultra-Fast\nAll-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"issue":"10","key":"2026072809091284700_cit54","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE:\nHighly Accurate OTU Sequences from Microbial Amplicon Reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"issue":"D1","key":"2026072809091284700_cit55","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA\nRibosomal RNA Gene Database Project: Improved Data Processing and\nWeb-Based Tools","volume":"41","author":"Quast","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2026072809091284700_cit56","doi-asserted-by":"crossref","unstructured":"Edgar, R. 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