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We addressed this gap using a fully reproducible approach on a widely cited data set of 101 small agricultural streams across Germany, where earlier data analyses have been subject to discussion, applying linear and penalized regression with bootstrap stability analysis. Pesticide toxicity remained consistently identified as a key stressor regardless of modeling approach. Summed toxic units combining event and grab sampling strengthened the pesticide-macroinvertebrate association compared to maximum toxic units or single sampling methods, suggesting that mixture-level exposure mechanisms and substance-specific toxicokinetics matter. Beyond pesticides, data aggregation choices influenced the relative importance of multiple stressors, with agricultural land use, nutrients, and hydromorphological degradation showing stronger effects than previously reported and no single stressor dominating the ecological responses. Different ecological metrics responded to distinct stressor sets, highlighting the metric choice as a relevant consideration in interpreting multiple stressor effects. Our findings reveal that conclusions about dominant stressors can be sensitive to analytical decisions, calling for transparent, multimetric, multimodel approaches to enable more defensible evaluations of chemical mixture and multiple stressor effects on freshwater biodiversity.<\/jats:p>","DOI":"10.1021\/acs.est.6c06517","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T07:18:31Z","timestamp":1788333511000},"page":"25753-25762","source":"Crossref","is-referenced-by-count":1,"title":["Data Analysis Choices\nInfluence the Relative Importance\nof Multiple Stressors on Macroinvertebrates in Agricultural Streams"],"prefix":"10.1021","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7955-9744","authenticated-orcid":true,"given":"Hanh H.","family":"Nguyen","sequence":"first","affiliation":[{"name":"University of Duisburg-Essen , , Universita\u0308tsstrasse 2 , ,","place":["Essen, Germany, 45141"]},{"name":"University Alliance Ruhr , , Universita\u0308tsstrasse 2 , ,","place":["Essen, Germany, 45141"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8732-3766","authenticated-orcid":true,"given":"Verena C.","family":"Schreiner","sequence":"additional","affiliation":[{"name":"University of Duisburg-Essen , , Universita\u0308tsstrasse 2 , ,","place":["Essen, Germany, 45141"]},{"name":"University Alliance Ruhr , , Universita\u0308tsstrasse 2 , ,","place":["Essen, Germany, 45141"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3510-1701","authenticated-orcid":true,"given":"Ralf B.","family":"Scha\u0308fer","sequence":"additional","affiliation":[{"name":"University of Duisburg-Essen , , Universita\u0308tsstrasse 2 , ,","place":["Essen, Germany, 45141"]},{"name":"University Alliance Ruhr , , Universita\u0308tsstrasse 2 , ,","place":["Essen, Germany, 45141"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"issue":"8049","key":"2026091511115192000_cit1","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1038\/s41586-024-08375-z","article-title":"One-Quarter\nof Freshwater Fauna Threatened with Extinction","volume":"638","author":"Sayer","year":"2025","journal-title":"Nature"},{"issue":"21","key":"2026091511115192000_cit2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216573120","article-title":"Farmland Practices Are Driving Bird\nPopulation Decline across Europe","volume":"120","author":"Rigal","year":"2023","journal-title":"Proc. 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