{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:39:52Z","timestamp":1790815192734,"version":"4.1.0"},"reference-count":60,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-EQPX-27-01"],"award-info":[{"award-number":["ANR-10-EQPX-27-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004617","name":"Institut National des Sciences de l'Univers, Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004617","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000160","name":"Division of Earth Sciences","doi-asserted-by":"publisher","award":["EAR-1424899"],"award-info":[{"award-number":["EAR-1424899"]}],"id":[{"id":"10.13039\/100000160","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006489","name":"Commissariat ? l'?nergie Atomique et aux ?nergies Alternatives","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006489","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Geosciences, Princeton University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The conversion of inorganic mercury (Hg\u00ad(II)) to methylmercury (MeHg) is central to the understanding of Hg toxicity in the environment. Hg methylation occurs in the cytosol of certain obligate anaerobic bacteria and archaea possessing the hgcAB gene cluster. However, the processes involved in Hg\u00ad(II) biouptake and methylation are not well understood. Here, we examined the role of cell surface thiols, cellular ligands with the highest affinity for Hg\u00ad(II) that are located at the interface between the outer membrane and external medium, on the sorption and methylation of Hg\u00ad(II) by Geobacter sulfurreducens. The effect of added cysteine (Cys), which is known to greatly enhance Hg\u00ad(II) biouptake and methylation, was also explored. By quantitatively blocking surface thiols with a thiol binding ligand (qBBr), we show that surface thiols have no significant effect on Hg\u00ad(II) methylation, regardless of Cys addition. The results also identify a significant amount of cell-associated Hg-S3\/S4 species, as studied by high energy-resolution X-ray absorption near edge structure (HR-XANES) spectroscopy, under conditions of high MeHg production (with Cys addition). In contrast, Hg-S2 are the predominant species during low MeHg production. Hg-S3\/S4 species may be related to enhanced Hg\u00ad(II) biouptake or the ability of Hg\u00ad(II) to become methylated by HgcAB and should be further explored in this context.<\/jats:p>","DOI":"10.1021\/acs.est.9b05915","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T18:50:37Z","timestamp":1582656637000},"page":"3960-3968","source":"Crossref","is-referenced-by-count":42,"title":["Cellular\nMercury Coordination Environment, and Not\nCell Surface Ligands, Influence Bacterial Methylmercury Production"],"prefix":"10.1021","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4970-4431","authenticated-orcid":true,"given":"Sara A.","family":"Thomas","sequence":"first","affiliation":[{"name":"Princeton University , , Guyot Hall , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhoopesh","family":"Mishra","sequence":"additional","affiliation":[{"name":"University of Leeds , , ,","place":["Leeds, U.K., LS2 9JT"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish C.B.","family":"Myneni","sequence":"additional","affiliation":[{"name":"Princeton University , , Guyot Hall , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,2,25]]},"reference":[{"issue":"2","key":"2026081715270928900_cit1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02403-17","article-title":"Robust\nmercury methylation across diverse methanogenic archaea","volume":"9","author":"Gilmour","year":"2018","journal-title":"mBio"},{"issue":"9","key":"2026081715270928900_cit2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1500675","article-title":"Global prevalence and distribution of genes and microorganisms\ninvolved in mercury methylation","volume":"1","author":"Podar","year":"2015","journal-title":"Sci. Adv."},{"issue":"20","key":"2026081715270928900_cit3","doi-asserted-by":"publisher","first-page":"11810","DOI":"10.1021\/es403075t","article-title":"Mercury\nmethylation by novel microorganisms from new\nenvironments","volume":"47","author":"Gilmour","year":"2013","journal-title":"Environ. Sci. Technol."},{"issue":"6125","key":"2026081715270928900_cit4","doi-asserted-by":"publisher","first-page":"1332","DOI":"10.1126\/science.1230667","article-title":"The genetic\nbasis for bacterial mercury\nmethylation","volume":"339","author":"Parks","year":"2013","journal-title":"Science"},{"issue":"7","key":"2026081715270928900_cit5","doi-asserted-by":"publisher","first-page":"3678","DOI":"10.1021\/acs.est.6b04622","article-title":"Thermodynamic modeling of the solubility\nand chemical speciation of mercury and methylmercury driven by organic\nthiols and micromolar sulfide concentrations in boreal wetland soils","volume":"51","author":"Liem-Nguyen","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"2026081715270928900_cit6","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1146\/annurev.ecolsys.29.1.543","article-title":"The chemical\ncycle\nand bioaccumulation of mercury","volume":"29","author":"Morel","year":"1998","journal-title":"Annu. Rev. Ecol.\nSyst."},{"key":"2026081715270928900_cit7","doi-asserted-by":"publisher","first-page":"G00C03","DOI":"10.1029\/2008JG000745","article-title":"Competition\namong thiols and inorganic sulfides and polysulfides for Hg and MeHg\nin wetland soils and sediments under suboxic conditions: Illumination\nof controversies and implications for MeHg net production","volume":"113","author":"Skyllberg","year":"2008","journal-title":"J. Geophys. Res.-Biogeo."},{"issue":"1","key":"2026081715270928900_cit8","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1128\/AEM.67.1.51-58.2001","article-title":"Aspects of bioavailability of mercury\nfor methylation in pure cultures of Desulfobulbus propionicus (1pr3)","volume":"67","author":"Benoit","year":"2001","journal-title":"Appl. Environ. Microb."},{"issue":"1","key":"2026081715270928900_cit9","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1021\/es001415n","article-title":"The influence\nof sulfide on solid\nphase mercury bioavailability for methylation by pure cultures of Desulfobulbus propionicus (1pr3)","volume":"35","author":"Benoit","year":"2001","journal-title":"Environ. Sci. Technol."},{"issue":"6","key":"2026081715270928900_cit10","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1021\/es9808200","article-title":"Sulfide controls on\nmercury speciation and bioavailability to methylating bacteria in\nsediment pore waters","volume":"33","author":"Benoit","year":"1999","journal-title":"Environ. Sci. Technol."},{"issue":"7","key":"2026081715270928900_cit11","doi-asserted-by":"publisher","first-page":"2270","DOI":"10.1021\/es061724z","article-title":"Importance\nof dissolved neutral mercury sulfides for methyl mercury production\nin contaminated sediments","volume":"41","author":"Drott","year":"2007","journal-title":"Environ. Sci. Technol."},{"issue":"5","key":"2026081715270928900_cit12","doi-asserted-by":"publisher","first-page":"3007","DOI":"10.1021\/es405215v","article-title":"Effect of divalent metals on Hg\u00ad(II)\nuptake and methylation by bacteria","volume":"48","author":"Schaefer","year":"2014","journal-title":"Environ.\nSci. Technol."},{"issue":"21","key":"2026081715270928900_cit13","doi-asserted-by":"publisher","first-page":"8714","DOI":"10.1073\/pnas.1105781108","article-title":"Active transport, substrate specificity, and methylation\nof Hg\u00ad(II) in anaerobic bacteria","volume":"108","author":"Schaefer","year":"2011","journal-title":"Proc. Natl.\nAcad. Sci. U. S. A."},{"issue":"2","key":"2026081715270928900_cit14","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/ngeo412","article-title":"High methylation\nrates of mercury bound to cysteine\nby Geobacter sulfurreducens","volume":"2","author":"Schaefer","year":"2009","journal-title":"Nat. Geosci."},{"issue":"5","key":"2026081715270928900_cit15","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1021\/acs.estlett.5b00068","article-title":"Cysteine inhibits mercury methylation\nby Geobacter sulfurreducensPCA mutant omcBESTZ","volume":"2","author":"Lin","year":"2015","journal-title":"Environ. Sci. Technol. Lett."},{"issue":"7","key":"2026081715270928900_cit16","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1039\/C9MT00077A","article-title":"The role of cysteine and sulfide\nin the interplay between\nmicrobial Hg\u00ad(II) uptake and sulfur metabolism","volume":"11","author":"Thomas","year":"2019","journal-title":"Metallomics"},{"issue":"17","key":"2026081715270928900_cit17","doi-asserted-by":"publisher","first-page":"10030","DOI":"10.1021\/acs.est.8b01305","article-title":"Spectroscopic\nand microscopic evidence of biomediated HgS species formation from\nHg\u00ad(II)-cysteine complexes: Implications for Hg\u00ad(II) bioavailability","volume":"52","author":"Thomas","year":"2018","journal-title":"Environ. Sci. Technol."},{"issue":"8","key":"2026081715270928900_cit18","doi-asserted-by":"publisher","first-page":"4642","DOI":"10.1021\/acs.est.6b06400","article-title":"Cysteine addition\npromotes sulfide production and 4-fold\nHg\u00ad(II)-S coordination in actively metabolizing Escherichia\ncoli","volume":"51","author":"Thomas","year":"2017","journal-title":"Environ. Sci. Technol."},{"issue":"5","key":"2026081715270928900_cit19","doi-asserted-by":"publisher","first-page":"2715","DOI":"10.1021\/es203658f","article-title":"Dissolved organic matter enhances\nmicrobial mercury methylation under sulfidic conditions","volume":"46","author":"Graham","year":"2012","journal-title":"Environ. Sci. Technol."},{"issue":"14","key":"2026081715270928900_cit20","doi-asserted-by":"publisher","first-page":"8187","DOI":"10.1021\/acs.est.9b01502","article-title":"Microbial biosynthesis of thiol compounds: Implications\nfor speciation, cellular uptake, and methylation of Hg\u00ad(II)","volume":"53","author":"Adediran","year":"2019","journal-title":"Environ. Sci. Technol."},{"issue":"12","key":"2026081715270928900_cit21","doi-asserted-by":"publisher","first-page":"2213","DOI":"10.1039\/C4MT00172A","article-title":"Hg\u00ad(II)\nbacterial biouptake: The role\nof anthropogenic and biogenic ligands present in solution and spectroscopic\nevidence of ligand exchange reactions at the cell surface","volume":"6","author":"Thomas","year":"2014","journal-title":"Metallomics"},{"key":"2026081715270928900_cit22","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.chemgeo.2017.02.015","article-title":"Stoichiometry\nof mercury-thiol complexes on bacterial\ncell envelopes","volume":"464","author":"Mishra","year":"2017","journal-title":"Chem. Geol."},{"key":"2026081715270928900_cit23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.gca.2014.11.018","article-title":"The effect of natural\norganic matter on the adsorption of mercury to bacterial cells","volume":"150","author":"Dunham-Cheatham","year":"2015","journal-title":"Geochim. Cosmochim. Acta"},{"key":"2026081715270928900_cit24","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.chemgeo.2014.02.030","article-title":"The effect\nof chloride on the adsorption of Hg onto three bacterial species","volume":"373","author":"Dunham-Cheatham","year":"2014","journal-title":"Chem. Geol."},{"key":"2026081715270928900_cit25","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1016\/j.gca.2013.03.001","article-title":"Anaerobic\noxidation of Hg(0) and methylmercury formation by Desulfovibrio\ndesulfuricansND132","volume":"112","author":"Colombo","year":"2013","journal-title":"Geochim. Cosmochim.\nActa"},{"issue":"4","key":"2026081715270928900_cit26","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1002\/chem.201804209","article-title":"Frontispiece: Mercury\u00ad(II) binding to metallothionein in Mytilus\nedulis revealed by high energy-resolution XANES spectroscopy","volume":"25","author":"Manceau","year":"2019","journal-title":"Chem. - Eur. J."},{"issue":"9","key":"2026081715270928900_cit27","doi-asserted-by":"publisher","first-page":"4880","DOI":"10.1021\/acs.est.8b06579","article-title":"Divalent mercury\nin dissolved organic matter is bioavailable\nto fish and accumulates as dithiolate and tetrathiolate complexes","volume":"53","author":"Bourdineaud","year":"2019","journal-title":"Environ. Sci. Technol."},{"issue":"18","key":"2026081715270928900_cit28","doi-asserted-by":"publisher","first-page":"10286","DOI":"10.1021\/acs.est.8b02027","article-title":"Chemical forms\nof mercury in pyrite: Implications for predicting mercury releases\nin acid mine drainage settings","volume":"52","author":"Manceau","year":"2018","journal-title":"Environ. Sci.\nTechnol."},{"issue":"19","key":"2026081715270928900_cit29","doi-asserted-by":"publisher","first-page":"10721","DOI":"10.1021\/acs.est.6b03468","article-title":"Chemical forms of mercury in human hair reveal sources\nof exposure","volume":"50","author":"Manceau","year":"2016","journal-title":"Environ. Sci. Technol."},{"issue":"16","key":"2026081715270928900_cit30","doi-asserted-by":"publisher","first-page":"9787","DOI":"10.1021\/acs.est.5b02522","article-title":"Formation of mercury\nsulfide from Hg\u00ad(II)-thiolate complexes in natural organic matter","volume":"49","author":"Manceau","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"2026081715270928900_cit31","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.chemgeo.2019.04.026","article-title":"Bacterial cell envelope and extracellular\nsulfhydryl binding sites: Their roles in metal binding and bioavailability","volume":"521","author":"Fein","year":"2019","journal-title":"Chem. Geol."},{"issue":"22","key":"2026081715270928900_cit32","doi-asserted-by":"publisher","first-page":"9597","DOI":"10.1021\/es201820c","article-title":"Binding of Hg-II\nto high-affinity sites on bacteria inhibits reduction to Hg-0 by mixed\nFe-II\/III phases","volume":"45","author":"Mishra","year":"2011","journal-title":"Environ. Sci. Technol."},{"issue":"11","key":"2026081715270928900_cit33","doi-asserted-by":"publisher","first-page":"1721","DOI":"10.1016\/j.biochi.2006.09.008","article-title":"Bioavailability\nof trace metals to aquatic microorganisms: Importance of chemical,\nbiological and physical processes on biouptake","volume":"88","author":"Worms","year":"2006","journal-title":"Biochimie"},{"issue":"18","key":"2026081715270928900_cit34","doi-asserted-by":"publisher","first-page":"9854","DOI":"10.1021\/es301381n","article-title":"Estimation\nof reactive thiol concentrations in dissolved organic matter and bacterial\ncell membranes in aquatic systems","volume":"46","author":"Joe-Wong","year":"2012","journal-title":"Environ.\nSci. Technol."},{"issue":"6","key":"2026081715270928900_cit35","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.fuproc.2003.11.003","article-title":"Zeeman atomic absorption spectrometer RA-915+ for direct\ndetermination of mercury in air and complex matrix samples","volume":"85","author":"Sholupov","year":"2004","journal-title":"Fuel Process. Technol."},{"key":"2026081715270928900_cit36","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1107\/S0909049505012719","article-title":"Athena, artemis, hephaestus:\nData analysis for X-ray\nabsorption spectroscopy using IFEFFIT","volume":"12","author":"Ravel","year":"2005","journal-title":"J. Synchrotron\nRadiat."},{"key":"2026081715270928900_cit37","doi-asserted-by":"publisher","first-page":"012007","DOI":"10.1088\/1742-6596\/430\/1\/012007","article-title":"Larch: An\nanalysis package for XAFS and related spectroscopies","volume":"430","author":"Newville","year":"2013","journal-title":"J. Phys.: Conf. Ser."},{"issue":"20","key":"2026081715270928900_cit38","doi-asserted-by":"publisher","first-page":"11564","DOI":"10.1021\/acs.est.8b01987","article-title":"Adsorption\nof methylmercury onto Geobacter bemidijensisBem","volume":"52","author":"Wang","year":"2018","journal-title":"Environ. Sci. Technol."},{"issue":"24","key":"2026081715270928900_cit39","doi-asserted-by":"publisher","first-page":"14360","DOI":"10.1021\/acs.est.7b04784","article-title":"Enhanced removal\nof dissolved Hg\u00ad(II), Cd\u00ad(II), and Au\u00ad(III)\nfrom water by Bacillus subtilis bacterial biomass\ncontaining an elevated concentration of sulfhydryl sites","volume":"51","author":"Yu","year":"2017","journal-title":"Environ. Sci. Technol."},{"issue":"11","key":"2026081715270928900_cit40","doi-asserted-by":"publisher","first-page":"5498","DOI":"10.1021\/acs.est.6b00347","article-title":"Sulfhydryl binding\nsites within bacterial extracellular\npolymeric substances","volume":"50","author":"Yu","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"2026081715270928900_cit41","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.chemgeo.2014.02.027","article-title":"Characterization\nof sulfhydryl sites within bacterial cell envelopes using selective\nsite-blocking and potentiometric titrations","volume":"373","author":"Yu","year":"2014","journal-title":"Chem. Geol."},{"issue":"19","key":"2026081715270928900_cit42","doi-asserted-by":"publisher","first-page":"10922","DOI":"10.1021\/es400527m","article-title":"Mercury reduction and cell-surface\nadsorption by Geobacter sulfurreducensPCA","volume":"47","author":"Hu","year":"2013","journal-title":"Environ. Sci. Technol."},{"issue":"6","key":"2026081715270928900_cit43","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.2134\/jeq2017.01.0023","article-title":"High-energy resolution fluorescence\ndetected X-ray absorption spectroscopy: A powerful new structural\ntool in environmental biogeochemistry sciences","volume":"46","author":"Proux","year":"2017","journal-title":"J. Environ. Qual."},{"issue":"24","key":"2026081715270928900_cit44","doi-asserted-by":"publisher","first-page":"11776","DOI":"10.1021\/acs.inorgchem.5b01932","article-title":"Structure,\nbonding, and stability of mercury complexes with thiolate and thioether\nligands from high-resolution XANES spectroscopy and first-principles\ncalculations","volume":"54","author":"Manceau","year":"2015","journal-title":"Inorg. Chem."},{"issue":"5\u20136","key":"2026081715270928900_cit45","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1089\/ars.2006.8.753","article-title":"The many faces of glutathione in\nbacteria","volume":"8","author":"Masip","year":"2006","journal-title":"Antioxid. Redox Signaling"},{"issue":"16","key":"2026081715270928900_cit46","doi-asserted-by":"publisher","first-page":"4693","DOI":"10.1016\/j.gca.2010.05.012","article-title":"Rate of\nformation and dissolution of mercury sulfide nanoparticles: The dual\nrole of natural organic matter","volume":"74","author":"Slowey","year":"2010","journal-title":"Geochim. Cosmochim.\nActa"},{"issue":"22","key":"2026081715270928900_cit47","doi-asserted-by":"publisher","first-page":"13133","DOI":"10.1021\/acs.est.7b02687","article-title":"Effects of sulfide concentration\nand dissolved organic matter characteristics on the structure of nanocolloidal\nmetacinnabar","volume":"51","author":"Poulin","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"2026081715270928900_cit48","doi-asserted-by":"publisher","first-page":"39359","DOI":"10.1038\/srep39359","article-title":"Nucleation of mercury sulfide by\ndealkylation","volume":"6","author":"Enescu","year":"2016","journal-title":"Sci. Rep."},{"issue":"3","key":"2026081715270928900_cit49","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.1007\/s00214-014-1457-x","article-title":"High-level ab initio calculation of the stability of\nmercury-thiolate complexes","volume":"133","author":"Enescu","year":"2014","journal-title":"Theor. Chem. Acc."},{"issue":"7","key":"2026081715270928900_cit50","doi-asserted-by":"publisher","first-page":"3935","DOI":"10.1021\/acs.est.7b05452","article-title":"Biogenesis\nof mercury-sulfur nanoparticles in plant leaves from atmospheric gaseous\nmercury","volume":"52","author":"Manceau","year":"2018","journal-title":"Environ. Sci. Technol."},{"issue":"6","key":"2026081715270928900_cit51","doi-asserted-by":"publisher","first-page":"2434","DOI":"10.1021\/ja00162a062","article-title":"Coordination\nchemistry of the Hg-MerR metalloregulatory protein - evidence for\na novel tridentate Hg-cysteine receptor-site","volume":"112","author":"Wright","year":"1990","journal-title":"J. Am. Chem. Soc."},{"key":"2026081715270928900_cit52","first-page":"103","article-title":"Chapter Five\n- Modeling Mercury in Proteins","volume-title":"Methods\nin Enzymology","author":"Parks","year":"2016"},{"issue":"9","key":"2026081715270928900_cit53","doi-asserted-by":"publisher","first-page":"3205","DOI":"10.1128\/AEM.00217-15","article-title":"Site-directed\nmutagenesis of HgcA and HgcB reveals amino acid residues important\nfor mercury methylation","volume":"81","author":"Smith","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"issue":"2","key":"2026081715270928900_cit54","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1021\/ic401992y","article-title":"Mercury\nmethylation by HgcA: Theory supports carbanion transfer to Hg\u00ad(II)","volume":"53","author":"Zhou","year":"2014","journal-title":"Inorg. Chem."},{"issue":"10","key":"2026081715270928900_cit55","doi-asserted-by":"publisher","first-page":"3540","DOI":"10.1021\/acs.jproteome.6b00263","article-title":"Global proteome response\nto deletion of genes related to mercury methylation and dissimilatory\nmetal reduction reveals changes in respiratory metabolism in Geobacter sulfurreducensPCA","volume":"15","author":"Qian","year":"2016","journal-title":"J. Proteome\nRes."},{"issue":"13","key":"2026081715270928900_cit56","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00438-19","article-title":"Kinetics of enzymatic mercury methylation\nat nanomolar concentrations\ncatalyzed by HgcAB","volume":"85","author":"Date","year":"2019","journal-title":"Appl. Environ. Microbiol."},{"issue":"18","key":"2026081715270928900_cit57","doi-asserted-by":"publisher","first-page":"13764","DOI":"10.1007\/s11356-015-4273-5","article-title":"Relationships between bacterial energetic\nmetabolism, mercury methylation potential, and hgcA\/hgcB gene expression in Desulfovibrio dechloroacetivoransBerOc1","volume":"22","author":"Gon\u0303i-Urriza","year":"2015","journal-title":"Environ. Sci. Pollut. Res."},{"issue":"24","key":"2026081715270928900_cit58","doi-asserted-by":"publisher","first-page":"13335","DOI":"10.1021\/acs.est.6b04041","article-title":"Effects of cellular sorption on mercury\nbioavailability and methylmercury production by Desulfovibrio\ndesulfuricansND132","volume":"50","author":"Liu","year":"2016","journal-title":"Environ. Sci. Technol."},{"issue":"7","key":"2026081715270928900_cit59","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1021\/acsearthspacechem.9b00039","article-title":"Mercury adsorption\non minerals and its effect on microbial methylation","volume":"3","author":"Zhao","year":"2019","journal-title":"ACS Earth Space Chem."},{"issue":"20","key":"2026081715270928900_cit60","doi-asserted-by":"publisher","first-page":"7337","DOI":"10.1128\/AEM.01792-12","article-title":"Detailed\nassessment of the kinetics of Hg-cell association, Hg methylation,\nand methylmercury degradation in several Desulfovibrio species","volume":"78","author":"Graham","year":"2012","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Environmental Science &amp; Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.est.9b05915","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/esthag\/article-pdf\/54\/7\/3960\/10298318\/es9b05915.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/esthag\/article-pdf\/54\/7\/3960\/10298318\/es9b05915.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:27:24Z","timestamp":1786994844000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/esthag\/article\/54\/7\/3960\/974441\/Cellular-Mercury-Coordination-Environment-and-Not"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,25]]},"references-count":60,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,2,25]]},"published-print":{"date-parts":[[2020,4,7]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.est.9b05915","relation":{},"ISSN":["0013-936X","1520-5851"],"issn-type":[{"value":"0013-936X","type":"print"},{"value":"1520-5851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,25]]}}}