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In vitro assays identified compound L5 as the most active agent, with an EC50 of 10.797 \u03bcg\/mL (2-fold higher than DSA), and pot experiments confirmed its efficacy against cotton damping-off disease. Mechanistic investigations via electron microscopy, transcriptomics, physiological assays and molecular docking suggest that L5 may target fatty acid synthase (FAS) to interfere with fungal lipid biosynthesis, disrupt energy homeostasis, and induce oxidative stress, which triggers synergistic membrane impairment and oxidative damage and ultimately causes hyphal death. With a novel target and a distinctive single-target, multieffect mode of action, L5 is a promising candidate for new fungicide development.<\/jats:p>","DOI":"10.1021\/acs.jafc.5c15094","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:31:00Z","timestamp":1788402660000},"page":"28112-28123","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis of 4-(Diethylamino)salicylaldehyde\nDerivatives\nand Research on Their Antifungal Mechanism against\n                    <i>Rhizoctonia solani<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Yaofeng","family":"He","sequence":"first","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyang","family":"Shi","sequence":"additional","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Zeeshan","sequence":"additional","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4433-6003","authenticated-orcid":true,"given":"Guoqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiang","family":"Han","sequence":"additional","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Ma","sequence":"additional","affiliation":[{"name":"Shihezi University , , ,","place":["Shihezi, China, 832003"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"2026091609094776500_cit1","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/s10526-021-10123-5","article-title":"Bacteria in the genus Streptomyces are effective biological control\nagents for management of fungal plant pathogens: A meta-analysis","volume":"67","author":"Le Blanc","year":"2022","journal-title":"BioControl"},{"key":"2026091609094776500_cit2","doi-asserted-by":"publisher","first-page":"881116","DOI":"10.3389\/fpls.2022.881116","article-title":"Rhizoctonia solani Ku\u0308hn\npathophysiology: Status and prospects of sheath blight disease management\nin rice","volume":"13","author":"Senapati","year":"2022","journal-title":"Front. 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