{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:00:39Z","timestamp":1785942039785,"version":"3.56.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"30","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302629"],"award-info":[{"award-number":["32302629"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Funding of Wuhan Polytechnic University","award":["2023RZ011"],"award-info":[{"award-number":["2023RZ011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lipids are vital cellular components with structural, storage, signaling, and defensive functions, and lipid metabolism is critical to strawberry quality. However, the regulatory mechanism underlying lipid metabolism during strawberry development and ripening remains unclear. Although LEAFY COTYLEDON 2 (LEC2) regulates lipid metabolism, its function in strawberries is unknown. This study explored the role of FaLEC2 in strawberries. FaLEC2 is highly expressed during strawberry development. FaLEC2 overexpression delayed ripening, promoted the accumulation of phosphatidylcholines (PCs), including 1,2-dipalmitoyl-3-sn-phosphatidylcholine (DPPC) and PC (16:0\/18:3(9Z,12Z,15Z)), and linoleic acid (LA), upregulated FaLOX2.4\/2.5\/3, downregulated FaLOX5-like2, and regulated various transcription factors (TFs). Correlation analysis revealed associations among lipid metabolites and multiple regulatory genes. Yeast one-hybrid and dual-luciferase reporter assays preliminarily suggested a regulatory association between FaLEC2 and FaPLA1, identifying FaPLA1 as a putative direct target. These findings indicate that FaLEC2 modulates PCs and LA by regulating lipid-related genes, along with a set of candidate TFs, thereby contributing to the regulation of phospholipid and linoleic acid metabolism in strawberries.<\/jats:p>","DOI":"10.1021\/acs.jafc.5c16280","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T08:16:08Z","timestamp":1785140168000},"page":"23982-23992","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome and\nMetabolome Analyses Reveal the Role\nof FaLEC2 in Regulating Lipid Metabolism in Strawberry"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4947-3442","authenticated-orcid":true,"given":"Hongyan","family":"Lu","sequence":"first","affiliation":[{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiling","family":"Yu","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyan","family":"Li","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7196-7153","authenticated-orcid":true,"given":"Yang","family":"Yi","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]},{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxun","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]},{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6571-6059","authenticated-orcid":true,"given":"Ting","family":"Min","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]},{"name":"Wuhan Polytechnic University , , ,","place":["Wuhan, China, 430023"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"2026080510101050100_cit1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-021-00502-5","article-title":"Strawberry sweetness and consumer preference are enhanced\nby specific volatile compounds","volume":"8","author":"Fan","year":"2021","journal-title":"Hortic. 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Biochem."},{"key":"2026080510101050100_cit5","doi-asserted-by":"publisher","first-page":"6075","DOI":"10.1093\/jxb\/eraf307","article-title":"The transcription factor\nCnERF109 modulates cold tolerance\nthrough regulating \u03b1-linolenic acid biosynthesis in Qinghai\u2013Tibet Campeiostachys nutans Griseb","volume":"76","author":"Jiang","year":"2025","journal-title":"J. Exp.\nBot."},{"key":"2026080510101050100_cit6","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25084375","article-title":"PfbZIP85 transcription factor mediates\n\u03c9-3 fatty acid-enriched\noil Biosynthesis by down-regulating PfLPAT1B gene\nexpression in plant tissues","volume":"25","author":"Huang","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"2026080510101050100_cit7","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.16138","article-title":"Genome-wide association\nand RNA-seq analyses reveal a potential gene related to linolenic\nacid in soybean seeds","volume":"11","author":"Qin","year":"2023","journal-title":"PeerJ"},{"key":"2026080510101050100_cit8","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01604","article-title":"Soybean LEC2\nregulates subsets of\ngenes involved in controlling the biosynthesis and catabolism of seed\nstorage substances and seed development","volume":"8","author":"Manan","year":"2017","journal-title":"Front.\nPlant Sci."},{"key":"2026080510101050100_cit9","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1111\/tpj.16762","article-title":"Multiple transcription factors of Arabidopsis thaliana that are activated by LEAFY COTYLEDON\n2 regulate triacylglycerol biosynthesis","volume":"119","author":"Kim","year":"2024","journal-title":"Plant\nJ."},{"key":"2026080510101050100_cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2022.111188","article-title":"FaLEC2\nrepressing FaLOX2 promoter\ninvolved in the metabolism of LOX-derived volatiles during strawberry\nripening","volume":"303","author":"Lu","year":"2022","journal-title":"Sci. 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