{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:44:22Z","timestamp":1792784662404,"version":"4.5.0"},"reference-count":52,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31400242"],"award-info":[{"award-number":["31400242"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900242"],"award-info":[{"award-number":["31900242"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Commissioned Project of the Scientific Research Program of Hubei Provincial Department of Education","award":["B2024295"],"award-info":[{"award-number":["B2024295"]}],"id":[{"id":"https:\/\/ror.org\/05yaa9j15","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tomato (Solanum lycopersicum) \u03b2-mannanase is known as playing an important role in mannan degradation; however, its biological functions and transcriptional regulation remain poorly understood. Here, we characterized the roles of mannan-1,4-\u03b2-mannanase 2 (SlMAN2) in tomato development and salt stress tolerance. Results showed that SlMAN2 was preferentially expressed in seeds, stems, stamens, and stigmas. SlMAN2 overexpression significantly increased fruit and seed size, accelerated seed germination, and enhanced salt tolerance under 100 mM NaCl, whereas knockout mutants exhibited the opposite phenotypes. Notably, dual-luciferase assays and electrophoretic mobility shift assay (EMSA) demonstrated that the transcription factor SlDOF3.1 directly binds to the SlMAN2 promoter and activates its transcription, but SlMAN2 overexpression suppressed SlDOF3.1 expression, suggesting the existence of a negative feedback regulatory loop. Collectively, our findings reveal that SlMAN2 functions as a pleiotropic regulator coordinating tomato fruit and seed development with salt stress tolerance and uncover a SlDOF3.1\u2013SlMAN2 regulatory module underlying these processes.<\/jats:p>","DOI":"10.1021\/acs.jafc.5c17770","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:01:47Z","timestamp":1788858107000},"page":"28384-28394","source":"Crossref","is-referenced-by-count":0,"title":["A \u03b2-Mannanase Gene, MAN2, Coordinates\nTomato Fruit and Seed Development and Salt Stress Tolerance"],"prefix":"10.1021","volume":"74","author":[{"given":"Fei-Fei","family":"Li","sequence":"first","affiliation":[{"name":"Wuhan University of Bioengineering , , ,","place":["Wuhan, China, 430415"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7092-5636","authenticated-orcid":true,"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Wuhan University of Bioengineering , , ,","place":["Wuhan, China, 430415"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Gui","family":"Hou","sequence":"additional","affiliation":[{"name":"Wuhan University of Bioengineering , , ,","place":["Wuhan, China, 430415"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3741-3928","authenticated-orcid":true,"given":"Fei","family":"Xu","sequence":"additional","affiliation":[{"name":"Wuhan University of Bioengineering , , ,","place":["Wuhan, China, 430415"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"2026091609084885600_cit1","doi-asserted-by":"publisher","first-page":"R874","DOI":"10.1016\/j.cub.2017.05.050","article-title":"Seed dormancy\nand germination","volume":"27","author":"Penfield","year":"2017","journal-title":"Curr. 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Biochem."},{"key":"2026091609084885600_cit7","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1111\/nph.18091","article-title":"Modern mannan:\na hemicellulose\u2019s journey","volume":"234","author":"Voiniciuc","year":"2022","journal-title":"New Phytol."},{"key":"2026091609084885600_cit8","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1104\/pp.123.4.1235","article-title":"A germination-specific\nendo-\u03b2-mannanase\ngene is expressed in the micropylar endosperm cap of tomato seeds","volume":"123","author":"Nonogaki","year":"2000","journal-title":"Plant Physiol."},{"key":"2026091609084885600_cit9","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/s00253-008-1423-4","article-title":"An overview of mannan structure and mannan-degrading\nenzyme systems","volume":"79","author":"Moreira","year":"2008","journal-title":"Appl. Microbiol. 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Adv. Res."},{"issue":"15","key":"2026091609084885600_cit44","doi-asserted-by":"publisher","first-page":"1672","DOI":"10.1016\/j.jplph.2009.04.008","article-title":"Expression and location of endo-beta-mannanase\nduring the ripening of tomato fruit, and the relationship between\nits activity and softening","volume":"166","author":"Wang","year":"2009","journal-title":"J. Plant Physiol."},{"key":"2026091609084885600_cit45","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1093\/jxb\/erv490","article-title":"Seed vigour and crop establishment: extending performance\nbeyond adaptation","volume":"67","author":"Finch-Savage","year":"2016","journal-title":"J. Exp. 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