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Understanding its growth regulation is key for developing novel control strategies. This study reveals that the transcription factor Cubitus interruptus (SfCi) of S. frugiperda, an unstable nuclear protein with conserved C2H2 zinc finger domains, is directly targeted and negatively regulated by miR-7-5p. Knocking down Sfci via RNA interference induces metabolic reprogramming: it promotes triglyceride storage by enhancing adipocyte differentiation while inhibiting lipolysis, and simultaneously increases glucose utilization to fuel larval development. Molecularly, this shift upregulates adipogenesis genes and suppresses cell proliferation genes. These findings establish the miR-7-5p\/Ci regulatory module as a core regulator balancing lipid and glucose metabolism to coordinate energy allocation and development in S. frugiperda.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c00244","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:02:17Z","timestamp":1788879737000},"page":"28989-29002","source":"Crossref","is-referenced-by-count":0,"title":["The miR-7-5p\/Ci Regulates Fat Differentiation and\nEnergy Metabolism Reprogramming in\n                    <i>Spodoptera frugiperda<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Liting","family":"Bao","sequence":"first","affiliation":[{"name":"Shanxi University , , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanxi Provincial People\u2019s Hospital , , ,","place":["Taiyuan, China, 030012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqing","family":"He","sequence":"additional","affiliation":[{"name":"Shanxi University , , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8618-5955","authenticated-orcid":true,"given":"Wei","family":"Dong","sequence":"additional","affiliation":[{"name":"Shanxi University , , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xubo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi University , , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9575-7019","authenticated-orcid":true,"given":"Yuejun","family":"Fu","sequence":"additional","affiliation":[{"name":"Shanxi University , , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"issue":"1","key":"2026092310075021400_cit1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s11033-023-08986-1","article-title":"CRISPR\/Cas9:\nA Cutting-Edge Solution\nfor Combatting the Fall Armyworm, Spodoptera Frugiperda","volume":"51","author":"Gouda","year":"2024","journal-title":"Mol. 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Biol."},{"key":"2026092310075021400_cit42","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.pestbp.2019.10.009","article-title":"Fenoxycarb and Methoxyfenozide (RH-2485) Affected Development and\nChitin Synthesis through Disturbing Glycometabolism in Lymantria Dispar\nLarvae","volume":"163","author":"Zhang","year":"2020","journal-title":"Pestic. Biochem. Physiol."},{"issue":"1","key":"2026092310075021400_cit43","doi-asserted-by":"publisher","first-page":"10443","DOI":"10.1038\/s41467-025-65407-6","article-title":"Desat1-Mediated Lipid Homeostasis Mitigates 20E-Induced Lipotoxicity\nin Blood-Fed Mosquitoes","volume":"16","author":"Sun","year":"2025","journal-title":"Nat. Commun."},{"key":"2026092310075021400_cit44","unstructured":"Zeng, Y. Q.\n          \n          Research on\nthe mechanism and application of Ci genes in Hedgehog pathway on development\nof silkworm; Master\u2019s thesis; Southwest University, 2025."}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c00244","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/37\/28989\/68449511\/acs.jafc.6c00244.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/37\/28989\/68449511\/acs.jafc.6c00244.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:51:28Z","timestamp":1790175088000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/37\/28989\/5426422\/The-miR-7-5p-Ci-Regulates-Fat-Differentiation-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":44,"journal-issue":{"issue":"37","published-online":{"date-parts":[[2026,9,8]]},"published-print":{"date-parts":[[2026,9,23]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c00244","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,8]]}}}