{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:51:20Z","timestamp":1791982280041,"version":"4.4.0"},"reference-count":39,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272828"],"award-info":[{"award-number":["32272828"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Yunnan Province","doi-asserted-by":"publisher","award":["202405AF140133"],"award-info":[{"award-number":["202405AF140133"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Milk fat is a major determinant of dairy product quality, but the receptor mechanisms by which dietary isoflavones regulate mammary lipid metabolism remain unclear. We examined glycitin-induced lipid synthesis in goat mammary epithelial cells (GMECs) and the involvement of G protein-coupled receptor 3 (GPR3). Glycitin increased triglyceride accumulation, lipid droplet formation, and lipogenic gene expression. Docking and molecular dynamics suggested a plausible glycitin\u2013GPR3 interaction, while the increase in cyclic adenosine monophosphate (cAMP) was weakened by GPR3 knockdown. Glycitin also increased GPR3 expression and reduced MYC-associated factor X (MAX)-associated transcriptional repression. GPR3 knockdown reduced triglycerides, diacylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). These findings identify GPR3-associated signaling as a potential mechanism linking glycitin to enhanced milk fat synthesis in GMECs.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c00383","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:07:12Z","timestamp":1790694432000},"page":"31554-31567","source":"Crossref","is-referenced-by-count":0,"title":["Glycitin Targets\nthe Orphan Receptor GPR3 to Promote\nMilk Fat Synthesis in Goat Mammary Epithelial Cells"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2547-8436","authenticated-orcid":true,"given":"Liaoyu","family":"Fan","sequence":"first","affiliation":[{"name":"Northwest A&F University , , ,","place":["Yangling, China, 712100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chu","family":"Chen","sequence":"additional","affiliation":[{"name":"Northwest A&F University , , ,","place":["Yangling, China, 712100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoran","family":"Li","sequence":"additional","affiliation":[{"name":"Northwest A&F University , , ,","place":["Yangling, China, 712100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runyu","family":"Yang","sequence":"additional","affiliation":[{"name":"Northwest A&F University , , ,","place":["Yangling, China, 712100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"Kunming Longteng Biological Dairy Co., Ltd , ,","place":["Kunming, China, 650000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0476-7615","authenticated-orcid":true,"given":"Huaiping","family":"Shi","sequence":"additional","affiliation":[{"name":"Northwest A&F University , , ,","place":["Yangling, China, 712100"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"Pt 1","key":"2026101408090692800_cit1","doi-asserted-by":"publisher","first-page":"140427","DOI":"10.1016\/j.foodchem.2024.140427","article-title":"Comparative analysis\nof the fatty acid profiles in goat milk during different lactation\nperiods and their interactions with volatile compounds and metabolites","volume":"460","author":"Liao","year":"2024","journal-title":"Food Chem."},{"issue":"2","key":"2026101408090692800_cit2","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1111\/asj.12420","article-title":"Effect\nof short-chain fatty acids\non triacylglycerol accumulation, lipid droplet formation and lipogenic\ngene expression in goat mammary epithelial cells","volume":"87","author":"Sun","year":"2016","journal-title":"Anim Sci. 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Anim."},{"issue":"56","key":"2026101408090692800_cit5","doi-asserted-by":"publisher","first-page":"34276","DOI":"10.1039\/D0RA06977A","article-title":"Effect of\nsoybean milk fermented with Lactobacillus plantarum HFY01 isolated from yak yogurt on weight loss and lipid reduction\nin mice with obesity induced by a high-fat diet","volume":"10","author":"Li","year":"2020","journal-title":"RSC Adv."},{"issue":"3","key":"2026101408090692800_cit6","doi-asserted-by":"publisher","first-page":"722","DOI":"10.3390\/molecules26030722","article-title":"Natural Herbal Estrogen-Mimetics\n(Phytoestrogens) Promote the Differentiation of Fallopian Tube Epithelium\ninto Multi-Ciliated Cells via Estrogen Receptor Beta","volume":"26","author":"Zhu","year":"2021","journal-title":"Molecules"},{"key":"2026101408090692800_cit7","doi-asserted-by":"publisher","first-page":"1098715","DOI":"10.3389\/fnut.2023.1098715","article-title":"Sodium acetate\nregulates milk fat synthesis through the activation of GPR41\/GPR43\nsignaling pathway","volume":"10","author":"Qi","year":"2023","journal-title":"Front. 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